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Citations for Biotium Inc. :
1 - 50 of 560 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium, 30029). Cells were treated with 10 µL of drug-containing media ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Plant Biology 2021Quote: ... 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) from Biotium (CA, USA), Wortmannin (WM ...
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bioRxiv - Immunology 2020Quote: Cell death within tumor slices was assessed using a green-fluorescent caspase 3/7 probe that binds DNA upon cleavage by caspase 3/7 (Nucview, Biotium). 2.106 untransduced or EGFR CAR T cells were applied onto BxPC3 tumor slices that were subsequently incubated with 5μM of NucView dye ...
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bioRxiv - Cancer Biology 2020Quote: ... and 7 using CalceinAM (Biotium, CA; 148504-34-1), Ethidium Homodimer I (Biotium ...
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bioRxiv - Cell Biology 2023Quote: ... Wash Buffer with 1 mM ADVASEP-7 (Biotium; 70029), and finally Wash Buffer alone ...
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bioRxiv - Neuroscience 2023Quote: ... Biocytin (1-3 mg/mL, Biotium) was dissolved in the pipette solution for post-hoc identification of recorded cells.
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bioRxiv - Microbiology 2022Quote: ... NucView 488 fluorescent caspase-3/7 substrate (Biotium, Fremont, CA, USA) was added at a final concentration of 1.25µg/ml to visualize cells undergoing apoptosis ...
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bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
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bioRxiv - Neuroscience 2019Quote: ... then diluted to 6x) and run on agarose gels (1-2%) with 4% GelRed (Biotium, California, USA).
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bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The larvae were housed in 10 μM INK128 (or DMSO) containing water and at 36 hpa they were treated with 5 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Biotium, #10058) for 55 min ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorescent caspase 3/7 substrate for detecting apoptosis by live staining (Biotium) at 2 µM was prepared with dosing medium (M199 +2% v/v FBS) ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Molecular Biology 2023Quote: The free radical probe 2’,7’-dichlorodihydrofluoresceindiacetate (H2DCFDA) (Biotium, Fremont, CA) was used to quantify ROS ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 u ml−1 phalloidin CF555 (Biotium, 00040) were added together with the secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: 5 mg of RNA was incubated with 2.5 mg biotin-conjugated MTSEA (1 mg/ml) (Biotium 90066-1) in MTSEA buffer [20 mM HEPES pH 7.3 (Quality Biological A611-J848-10) ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with 1 μM calcein-AM (Biotium, cat no. 80011-3) for 10 mins ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 μl of 1 mg/ml Propidium Iodide (40017, Biotium). The cell solution was incubated for 30 minu at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... ADVASEP-7 (Biotium) was also resuspended at 500 μM in HBSS ...
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bioRxiv - Neuroscience 2024Quote: ... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Genomics 2022Quote: ... 7-AAD (Biotium, 40037) was stained to exclude dead cells ...
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bioRxiv - Microbiology 2021Quote: ... containing 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Biotium). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 hr at room temperature with 5% non-fat dry milk (Apex #20-241) or 5% BSA (Biotium #22013) in TBST (10 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 uL of 1 mM of SGC5 (Cat. number 70057, Biotium Inc., Fremont, CA) was injected into the AC of an enucleated mouse eye ...
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bioRxiv - Cell Biology 2022Quote: Calcium transients of cultured embryonic cardiomyocytes at DIV4-5 were measured after incubating myocytes with the calcium-sensitive fluorescence dye Fura2-AM (1 μM, Biotium, Cat: 50033-1) diluted in 0.02% Pluronic acid F-127/DMSO (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 5 μmol/L NucView™ 488 caspase-3 substrate (Biotium). Images were obtained in phase contrast and fluorescence mode using a x10 objective and an IncuCyte FLR time-lapse fluorescence microscope (Essen Bioscience) ...
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bioRxiv - Cell Biology 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (10µg/ml, Biotium, cat. number 40043) was used for nuclear staining ...
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bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of 0.5 µg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of µg/ml ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in DMEM 1% penicillin/streptomycin with 5 nM MitoView 533 (Biotium, #70055). Plates were incubated at 37°C for 30 min protected from light ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS (3 × 5 min) and treated with Trueblack (Biotium, Cat# 23007) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... 40 μl of 1X 4’,6-diamidino-2-phenylindole (DAPI; Biotium; Reagent Table) was added and the slides were sealed with a coverslip and clear nail polish.
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bioRxiv - Neuroscience 2020Quote: ... Neuro-DiI (7% in ethanol; Biotium) was slowly injected ...
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bioRxiv - Neuroscience 2020Quote: ... Neuro- DiI (7% in ethanol; Biotium) was slowly injected ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... and qualitatively evaluated by mixing ∼2 μL of each sample with 1 μL of GelRed (Biotium), and running it on a 0.7 % agarose gel for 30 minutes at 150 V ...
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bioRxiv - Microbiology 2022Quote: ... and qualitatively evaluated by mixing ~2 μL of each sample with 1 μL of GelRed (Biotium), and running it on a 0.7 % agarose gel for 30 minutes at 150 V ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in RPMI 1640 medium and stained for 5 min with 1 μg/ml of WGA-CF594 (Biotium). Gametogenesis was induced by XA at the concentration indicated for each experiment ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1 h at 37°C in mKRB supplemented with native coelenterazine (5 µM, BIOTIUM), and then incubated for 15 minutes in mKRB supplemented with either TPEN (500 µM ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were run on a 2% agarose gel supplemented with gel green dye (Biotium, 41005-1). A subset of F0 samples was submitted to GeneWiz for Sanger sequencing and analyzed using SnapGene software (SnapGene).
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated with CF350-conjugated streptavidin for 2 h (1:3000; Biotium, Inc., Fremont, CA) for fluorescent investigation of biocytin filled neurons ...