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Citations for Biotium Inc. :
51 - 100 of 491 citations for 6 Methylimidazo 2 1 b thiazole 5 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... 5% (v/v) EvaGreen (31000, Biotium) was added to the amplification mixture to monitor the progress of library amplification ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Neuroscience 2020Quote: Intracellular chloride measurements were performed using the N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide (MQAE) (Biotium) chloride sensor.
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bioRxiv - Synthetic Biology 2023Quote: ... BigBlock assemblies and PCR products were analyzed by electrophoresis on 1-2% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2020Quote: ... Ethidium Homodimer I (Biotium; 61926-22-5) per manufacturer protocol and expression of phenotypical markers was measured at day 5 via immunostaining ...
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bioRxiv - Physiology 2021Quote: ... with or without 5 µM dipicrylamine (Biotium), using a homebuilt multi-barrel flow pipe (see above) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Microbiology 2020Quote: ... and ethidiumhomodimer-2 (Biotium, California, USA), at a concentration of 2 μM and 4 μM respectively and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2019Quote: ... RNA integrity was evaluated by electrophoresis in denaturing agarose gel (1% agarose, 5% formamide, 1X TAE) stained with 3x Gel Red (Biotium Inc., CA, USA). The RNA was quantified using the Qubit RNA Assay Kit (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then washed once with NE solution and incubated in 5 µg/mL wheat germ agglutinin conjugated to Alexa Fluor 405 (Biotium cat#29028-1) diluted in NE solution for 2.5 minutes ...
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bioRxiv - Microbiology 2019Quote: ... was incubated in biotinylation buffer (50 mM HEPES [pH 7.5], 5 mM EDTA) and 5 μg MTSEA-biotin (Biotium #90066), rotating at room temperature in the dark for 30 min ...
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bioRxiv - Physiology 2023Quote: ... preparations were loaded with Ca2+ (Rhod-2 AM, 50 µL of 1 mg/mL in DMSO + 10% pluronic acid, Biotium, Hayward, CA) and voltage (Vm)-sensitive dyes (RH237 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells stably expressing mt-AEQ were harvested at a density of 500,000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.4/NaOH) and the aequorin was reconstituted by incubation with 2 µM native coelenterazine n (Biotium, BOT-10115-1) for 2.5 h at RT ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...
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bioRxiv - Cell Biology 2023Quote: ... and DAPI (5 μg/ml) (Biotium, Cat #BT-40043). Staining with DAPI was done for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg/ml of DAPI (Biotium; Cat #BT-40043) was used as a viability dye ...
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bioRxiv - Immunology 2023Quote: ... and NucView-488 at 5 μM (Biotium, Cat. 10402). NucView-488 was selected to visualize activation of Caspase-3 in apoptosis ...
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bioRxiv - Pathology 2021Quote: Lipofuscin present in the brain of 6 month-old mice was quenched with TrueBlackTM Lipofuscin Autofluorescence Quencher (Biotium). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the media was changed to RPMI 1640 without phenol red supplemented with 6 μg/mL propidium iodide (Biotium). Cells were infected at an MOI of 100 for 2 hours ...
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bioRxiv - Physiology 2021Quote: ... and Di-8-ANEPPS (Biotium, #61012, 2 µM) or Wheat Germ Agglutinin Alexa Fluor™-488 Conjugate (Invitrogen™ ...
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bioRxiv - Neuroscience 2022Quote: ... Pluronic F127 and Rhod-2/AM from Biotium, buprenorphine from Champion Alstoe Animal Health ...
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bioRxiv - Neuroscience 2020Quote: ... applying 5 μl of Fluorogold (Hydroxystilbamine FluoroGold, Biotium, Fremont, CA) to the area ...
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bioRxiv - Physiology 2022Quote: ... Sections were stained with RedDot 2 nuclear stain (Biotium) and WGA Oregon Green (Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... the IVT reaction was doped with 5-amino-allyl UTP (Biotium) at 1:10 (aminoallyl-UTP ...
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bioRxiv - Cancer Biology 2022Quote: ... using 2 μg/ml of 405-Alexa-NHS ester (Biotium).
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bioRxiv - Systems Biology 2019Quote: ... The cells were incubated with 5 µM of DCF-DA (Biotium #10058) for 30 min at 37°C MCF-10A prior to trypsinization ...
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bioRxiv - Bioengineering 2021Quote: ... stained with (5 μg/mL) CF488 Wheat Germ Agglutinin (WGA) Conjugates (Biotium), and washed 3 times with HBSS ...
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bioRxiv - Cell Biology 2022Quote: ... Permeabilized cells were incubated with 5 U/mL of fluorescent phalloidin (Biotium) in PBS for 20 min and washed with PBS.
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bioRxiv - Molecular Biology 2023Quote: ... 5 µl of ready to use nDiO solution ‘Cellbrite™ Green’ (Biotium) was added to 200 µl purified virus and incubated for 1 h on a rotation wheel at RT ...
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bioRxiv - Microbiology 2022Quote: ... Resulting reactions were run on 2% agarose gel with GelGreen (Biotium) before imaging using an EZ Dock Imager (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: The free radical probe 2’,7’-dichlorodihydrofluoresceindiacetate (H2DCFDA) (Biotium, Fremont, CA) was used to quantify ROS ...
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bioRxiv - Physiology 2023Quote: ... followed by donkey anti-rat CF750 (2 µg/mL, 20857, Biotium). Slides were imaged with the Slideview VS200 (Olympus ...
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bioRxiv - Genomics 2024Quote: ... DNA was loaded onto 2% agarose gel including 1X GelRed (Biotium).
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bioRxiv - Neuroscience 2024Quote: ... all sections were treated with TrueBlack lipofuscin autofluorescence quencher (5%; Biotium cat: 23007) for 90 seconds followed by a 3x5 minute rinse in 1M PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 5 μmol/L NucView™ 488 caspase-3 substrate (Biotium). Images were obtained in phase contrast and fluorescence mode using a x10 objective and an IncuCyte FLR time-lapse fluorescence microscope (Essen Bioscience) ...
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bioRxiv - Plant Biology 2024Quote: Calcofluor White solution: 5 mM Calcofluor White (Biotium, Cat. No. 29067, Fremont, CA, USA) to a final concentration of 50 µM in water.
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bioRxiv - Microbiology 2022Quote: ... 10µL of reaction was loaded onto 2% agarose gel with GelGreen (Biotium) and ran for 30 minutes at 100V and visualized using an EZ Dock Imager (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... F(ab’)2-goat anti-mouse IgG CF568 (Biotium Cat# 20109, RRID:AB_10557119), F(ab’)2-goat anti-rabbit IgG CF568 (Biotium Cat# 20099 ...
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bioRxiv - Cell Biology 2023Quote: ... F(ab’)2-goat anti-rabbit IgG CF568 (Biotium Cat# 20099, RRID:AB_10563029), F(ab’)2-goat anti-mouse IgG Alexa 555 (Thermo Fisher Scientific Cat# A-21425 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed after 5 days by XTT Cell Proliferation Assay (Biotium, Fremont, CA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS (3 × 5 min) and treated with Trueblack (Biotium, Cat# 23007) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2 minutes in TrueBlack Lipofuscin Autofluorescence Quencher (Biotium cat no. 23007) with 3 subsequent 1x PBS rinses before mounting and imaging.
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bioRxiv - Developmental Biology 2019Quote: ... DNA band was visualized on 2% agar gels stained with GelRed (41003, Biotium). All mammary gland tissues used in this paper are from female mice.
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bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing was performed using PCR-mixture-2 blue (AmpliSens, Russia) and EvaGreen (Biotium, USA) as a dye ...
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bioRxiv - Bioengineering 2021Quote: ... the products were analyzed using electrophoresis on a 2% agarose gel containing GelRed (Biotium) and visualized under UV light.
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bioRxiv - Microbiology 2021Quote: PCR products were checked in 2% agarose gels stained with GelRed™ (Biotium, USA) under UV light ...