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Citations for Biotium Inc. :
51 - 100 of 198 citations for 6 Methyl 3 5 heptadien 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Intracellular chloride measurements were performed using the N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide (MQAE) (Biotium) chloride sensor.
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bioRxiv - Cancer Biology 2020Quote: ... Ethidium Homodimer I (Biotium; 61926-22-5) per manufacturer protocol and expression of phenotypical markers was measured at day 5 via immunostaining ...
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bioRxiv - Physiology 2021Quote: ... with or without 5 µM dipicrylamine (Biotium), using a homebuilt multi-barrel flow pipe (see above) ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated one cochlea in HBSS containing annexin V-Alexa647 at a 1/25 dilution and CF488A-WGA (Biotium) at 20 μg/mL and the other cochlea in the same buffer supplemented with 100 μM benzamil for 25 min at RT in an orbital shaker protected from the light ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were blocked for one hour and were incubated with primary antibodies diluted in TrueBlack WB Antibody Diluent (Biotium) supplemented with 0.2% Tween-20 (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Microbiology 2020Quote: ... and ethidiumhomodimer-2 (Biotium, California, USA), at a concentration of 2 μM and 4 μM respectively and incubated for 30 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... the following reagents were used: NucView Caspase-3 enzyme substrates (Biotium), Idelalisib (Selleckchem) ...
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bioRxiv - Cancer Biology 2021Quote: The NucView Caspase-3 activity assay (Biotium, Cambridge Biosciences, Cambridge, UK) was used to confirm apoptosis observed by Hoechst 33423/PI staining in selected cell lines (RPMI 8226 and ADC-1) ...
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bioRxiv - Microbiology 2022Quote: ... NucView 488 fluorescent caspase-3/7 substrate (Biotium, Fremont, CA, USA) was added at a final concentration of 1.25µg/ml to visualize cells undergoing apoptosis ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium, 30029). Cells were treated with 10 µL of drug-containing media ...
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bioRxiv - Microbiology 2019Quote: ... was incubated in biotinylation buffer (50 mM HEPES [pH 7.5], 5 mM EDTA) and 5 μg MTSEA-biotin (Biotium #90066), rotating at room temperature in the dark for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... A paired sample design was used where one DNA extraction tube was treated with the chemical propidium monoazide (PMAxx Dye, 20mM in H2O from Biotium) which prevents amplification of exDNA ...
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bioRxiv - Genetics 2020Quote: ... Samples were pulse centrifuged up to 1200 rpm and split in two, where one part (n = 230, PMA treated samples) was added PMA dye (Biotium, USA to a final concentration of 50 µM ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions of each of the cell lines were separately labeled with either one or both of ViaFluor 488 SE and ViaFluor 405 SE fluorescent dyes (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 u ml−1 phalloidin CF555 (Biotium, 00040) were added together with the secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 hr at room temperature with 5% non-fat dry milk (Apex #20-241) or 5% BSA (Biotium #22013) in TBST (10 mM Tris ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorescent caspase 3/7 substrate for detecting apoptosis by live staining (Biotium) at 2 µM was prepared with dosing medium (M199 +2% v/v FBS) ...
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bioRxiv - Neuroscience 2024Quote: ... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
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bioRxiv - Cell Biology 2023Quote: ... and DAPI (5 μg/ml) (Biotium, Cat #BT-40043). Staining with DAPI was done for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg/ml of DAPI (Biotium; Cat #BT-40043) was used as a viability dye ...
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bioRxiv - Immunology 2023Quote: ... and NucView-488 at 5 μM (Biotium, Cat. 10402). NucView-488 was selected to visualize activation of Caspase-3 in apoptosis ...
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bioRxiv - Cancer Biology 2019Quote: ... Caspase activation was visualised using 1µM NucView 488 Caspase-3 Enzyme Substrate (Biotium). Cell viability was quantified using propidium iodide (1 μg/mL) ...
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bioRxiv - Biophysics 2019Quote: ... The gel was stained for 40 minutes in a solution 3 × GelRed (Biotium) and visualized using UV light.
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with 1 μM calcein-AM (Biotium, cat no. 80011-3) for 10 mins ...
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bioRxiv - Pathology 2021Quote: Lipofuscin present in the brain of 6 month-old mice was quenched with TrueBlackTM Lipofuscin Autofluorescence Quencher (Biotium). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the media was changed to RPMI 1640 without phenol red supplemented with 6 μg/mL propidium iodide (Biotium). Cells were infected at an MOI of 100 for 2 hours ...
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bioRxiv - Physiology 2021Quote: ... and Di-8-ANEPPS (Biotium, #61012, 2 µM) or Wheat Germ Agglutinin Alexa Fluor™-488 Conjugate (Invitrogen™ ...
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bioRxiv - Neuroscience 2022Quote: ... Pluronic F127 and Rhod-2/AM from Biotium, buprenorphine from Champion Alstoe Animal Health ...
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bioRxiv - Neuroscience 2020Quote: ... applying 5 μl of Fluorogold (Hydroxystilbamine FluoroGold, Biotium, Fremont, CA) to the area ...
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bioRxiv - Plant Biology 2021Quote: ... plants were fumigated with 20 μM DiBAC4(3) (Biotium, Fermont, CA; 49–52, 64), in 1.5 mM KCl buffer for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were allowed to cure protected from light at RT for at least one day before the short edges of the coverglass only were sealed with CoverGrip (Biotium #23005, Fremont, CA, USA).
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bioRxiv - Physiology 2022Quote: ... Sections were stained with RedDot 2 nuclear stain (Biotium) and WGA Oregon Green (Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... the IVT reaction was doped with 5-amino-allyl UTP (Biotium) at 1:10 (aminoallyl-UTP ...
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bioRxiv - Neuroscience 2023Quote: ... After 3 PBS washes slides were treated with 1x TrueBlack or 2x TrueBlack Plus (Biotium) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... using 2 μg/ml of 405-Alexa-NHS ester (Biotium).
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bioRxiv - Systems Biology 2019Quote: ... The cells were incubated with 5 µM of DCF-DA (Biotium #10058) for 30 min at 37°C MCF-10A prior to trypsinization ...
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bioRxiv - Bioengineering 2021Quote: ... stained with (5 μg/mL) CF488 Wheat Germ Agglutinin (WGA) Conjugates (Biotium), and washed 3 times with HBSS ...
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bioRxiv - Cell Biology 2022Quote: ... Permeabilized cells were incubated with 5 U/mL of fluorescent phalloidin (Biotium) in PBS for 20 min and washed with PBS.
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bioRxiv - Neuroscience 2023Quote: ... Cholera toxin subunit B (ctb)- 640 or -568 (5 mg/ml, Biotium) was injected in NAc medial shell and core (from bregma ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µl of ready to use nDiO solution ‘Cellbrite™ Green’ (Biotium) was added to 200 µl purified virus and incubated for 1 h on a rotation wheel at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... a reagent cocktail comprised of Hoechst 33342 and NucView 488 Caspase-3 reporter (Biotium, Cat# 30029) was added to each well for final concentrations of 1 μg/mL and 1 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was analyzed using the NucView® 488 caspase-3 assay kit (Biotium, Cat# 10402-T) which contains NucView® 488 Caspase-3 substrate ...
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bioRxiv - Microbiology 2022Quote: ... Resulting reactions were run on 2% agarose gel with GelGreen (Biotium) before imaging using an EZ Dock Imager (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: The free radical probe 2’,7’-dichlorodihydrofluoresceindiacetate (H2DCFDA) (Biotium, Fremont, CA) was used to quantify ROS ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2 μl of 1 mg/ml Propidium Iodide (40017, Biotium). The cell solution was incubated for 30 minu at room temperature in the dark ...
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bioRxiv - Physiology 2023Quote: ... followed by donkey anti-rat CF750 (2 µg/mL, 20857, Biotium). Slides were imaged with the Slideview VS200 (Olympus ...
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bioRxiv - Genomics 2024Quote: ... DNA was loaded onto 2% agarose gel including 1X GelRed (Biotium).
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bioRxiv - Neuroscience 2024Quote: ... all sections were treated with TrueBlack lipofuscin autofluorescence quencher (5%; Biotium cat: 23007) for 90 seconds followed by a 3x5 minute rinse in 1M PBS ...