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Citations for Biotium Inc. :
101 - 135 of 135 citations for 6 Fluoro 2 methylamino 4 1H pyrimidinone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The amplicons from each reaction were visualized on 2% agarose gel stained with RedsafeTM (Biotium, CA, USA). Capillary sequencing was performed using forward and reverse primers with the BIG dye terminator chemistry v3.1 (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were the run on a 2% agarose gel stained with GelRed (Biotium, Fremont, CA, USA), and visually inspected in UV lighting (Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... the chromatin was stained with RedDot™2 Far-Red Nuclear Stain (Biotium, 1:100 diluted in PBS), and the samples mounted using Vectashield Mounting Medium and analyzed on an FV1000 ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Amplified fragments were visualized in 2% agarose gels stained by GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA). While amplification of short fragments (~ 570 bp ...
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bioRxiv - Microbiology 2020Quote: ... wells were washed twice with fresh media before CAMHB containing the eDNA stain (EthHD-2 (1 μM; Biotium)) was added to the wells ...
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bioRxiv - Molecular Biology 2023Quote: ... purification of tachyzoites from cell debris by filtration though 3 µm filters and incubation of 2×105 extracellular parasites in 39µl of D10 with or without 30µM PMAxx (Biotium) for 15 min in shaking and darkness ...
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bioRxiv - Neuroscience 2024Quote: ... multi-shank neural probe (NeuroNexus A4×4-3mm-100-125-177-A16, Ann Arbor, MI, USA) pre-coated with DiI (Biotium, Fremont, CA, USA) for fluorescent detection of recording sites was advanced dorsoventrally at 5 µm/s through the IC using a hydraulic micropositioner (Model 650 ...
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Genetics 2020Quote: ... 6 μl of the PCR product was used for electrophoresis in Tris-Acetate EDTA running buffer at 120V for 45 min on a 2% agarose gel stained with GelRed (Biotium). Experiments were repeated twice to verify the results ...
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bioRxiv - Evolutionary Biology 2020Quote: Aliquots (2μl) of PCR products were run for 20 minutes at 80 volts in 2% agarose gels stained with GelRedTM (Biotium) and visualized in a UV light transilluminator Alpha Imager 3400 Documentation and Analysis System (Alpha Innotech ...
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bioRxiv - Neuroscience 2020Quote: ... USA) and the products were separated through electrophoresis on a 2% agarose gel stained with GelRed (Biotium, Fremont, CA, USA). The wild-type allele generated a single fragment of 460 bp and the mutant allele generated two fragments of 276 and 184 bp.
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bioRxiv - Microbiology 2021Quote: ... and the product was visualized on 2% agarose gels ran at 120V for 20 minutes and stained with GelGreen (Biotium). Primers used for probe amplification can be found in the Key Resource table..
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bioRxiv - Immunology 2021Quote: To measure apoptosis 200 µL/well NucViewTM 530 red solution (2 µM in phenol red free and serum free RMPI medium) (Biotium) was added for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were analyzed by separation of mutant and wild-type alleles on a 2% agarose gel stained with Gel Red (Biotium). Primer sequences are listed in Table S4.
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bioRxiv - Genetics 2021Quote: Success of PCR was determined by electrophoresis using a 2 % (w/v) agarose gel stained with GelRed® (Biotium, USA) and visualized under UV light ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4ul of PCR product was visualised by electrophoresis on a 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium).
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Synthetic Biology 2023Quote: ... BigBlock assemblies and PCR products were analyzed by electrophoresis on 1-2% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were analyzed on 2% agarose gel and visualized with the aid of GelGreen nucleic acid gel stain (Biotium).
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bioRxiv - Cell Biology 2024Quote: ... PCR products were identified after agarose gel electrophoresis (500 A/90 V) on a 1.5 % or 2 % agarose gel and staining with GelRed® (41003; Biotium).
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bioRxiv - Bioengineering 2024Quote: ... All samples are incubated at 37°C for 15 minutes and then run on a 2% TAE agarose gel with GelRed (Biotium). Gels were imaged at non-saturating intensities using a GeneGenius Imaging System (Syngene) ...
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bioRxiv - Microbiology 2020Quote: ... at a final concentration of 20 μg/ml and incubated in the dark for 2 min before detection or with CellBrite™ Fix 488 Membrane Dye (Biotium) at a final concentration of 10X (from a 1:1000 dilution ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The larvae were housed in 10 μM INK128 (or DMSO) containing water and at 36 hpa they were treated with 5 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Biotium, #10058) for 55 min ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reaction products were separated on a 2 % agarose gel electrophoresis and visualized with GelRed (nucleic acid gel stain; #41003, Biotium, USA).
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... preparations were loaded with Ca2+ (Rhod-2 AM, 50 µL of 1 mg/mL in DMSO + 10% pluronic acid, Biotium, Hayward, CA) and voltage (Vm)-sensitive dyes (RH237 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells stably expressing mt-AEQ were harvested at a density of 500,000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.4/NaOH) and the aequorin was reconstituted by incubation with 2 µM native coelenterazine n (Biotium, BOT-10115-1) for 2.5 h at RT ...
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bioRxiv - Bioengineering 2022Quote: ... Aliquots of detached cells (approximately 2×106) were incubated with a series of CF®640R succinimidyl ester-labeled (Biotium, USA, cat. 92108) ACE2 extracellular domain (AA Q18 – S740 ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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bioRxiv - Synthetic Biology 2023Quote: ... All platereader experiments were performed with 20 μL volumes and the reactions were further supplemented with 2% BSA and 0.25x EvaGreen (Biotium, stock: 20x in H2O) in an optical ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...
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bioRxiv - Microbiology 2020Quote: ... Amplification products were visualized by gel electrophoresis on 2% agarose gel stained with GelRed® Nucleic Acid Gel Stain (Biotium Inc., Fremont, CA, USA).