Labshake search
Citations for Biotium Inc. :
1 - 50 of 142 citations for 6 Chloro N methyl 5 nitro 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
-
bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: Intracellular chloride measurements were performed using the N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide (MQAE) (Biotium) chloride sensor.
-
bioRxiv - Microbiology 2021Quote: ... containing 0.5 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Biotium). Images were acquired on an LSM 700 confocal microscope (Zeiss ...
-
bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (10µg/ml, Biotium, cat. number 40043) was used for nuclear staining ...
-
bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of 0.5 µg/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... After nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (40043, Biotium) in IF buffer at a final concentration of µg/ml ...
-
bioRxiv - Genetics 2021Quote: ... 40 μl of 1X 4’,6-diamidino-2-phenylindole (DAPI; Biotium; Reagent Table) was added and the slides were sealed with a coverslip and clear nail polish.
-
bioRxiv - Plant Biology 2021Quote: ... 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) from Biotium (CA, USA), Wortmannin (WM ...
-
bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
-
bioRxiv - Cancer Biology 2023Quote: ... then were incubated for 10 minutes at 21 °C in 1 μM 2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM, Biotium) in PSS ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... MTSEA-biotin (N-biotinylaminoethyl-methanethiosulfonate, Biotium) (500 µM ...
-
bioRxiv - Microbiology 2022Quote: ... and n were from Biotium (Fremont, CA); and coelenterazine e ...
-
bioRxiv - Cell Biology 2020Quote: ... antibody labeling kit Mix-n-Stain CF640R (Biotium); heme (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... 16 xg at 4°C and the pellet resuspended and incubated in WGA488 stain (Biotium, 5 μg/mL, diluted in HBSS) for 30 minutes ...
-
bioRxiv - Genomics 2022Quote: ... Drop-n-Stain CF 488A Donkey Anti-Rabbit IgG (Biotium 20950), Drop-n-Stain CF 594 Donkey Anti-Rabbit IgG (Biotium 20951) ...
-
bioRxiv - Genomics 2022Quote: ... Drop-n-Stain CF 594 Donkey Anti-Rabbit IgG (Biotium 20951), IRDye 650 Goat anti-Mouse IgG Secondary Antibody (LI-COR Biosciences 926-65010) ...
-
bioRxiv - Biochemistry 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) was purchased from Biotium, CA ...
-
bioRxiv - Molecular Biology 2023Quote: ... 6 μL Fast Evagreen qPCR Master Mix (Biotium, 31003), 2 μL of nuclease-free water ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 5 μL of 5 μg/mL Propidium Iodide (Biotium) for cell death detection ...
-
bioRxiv - Systems Biology 2019Quote: ... Mix-n-Stain CF555 and CF640R antibody labeling kits were purchased from Biotium Inc ...
-
bioRxiv - Genetics 2022Quote: ... and mounted with Drop-n-Stain EverBrite Mounting Medium with DAPI (BIOTIUM: 23009). All specimens were imaged using Zeiss Axio Imager.M1 fluorescence microscope with a 63X plan-apo Chromat oil objective ...
-
bioRxiv - Neuroscience 2023Quote: ... Retinae were then mounted in Drop-n-Stain EverBriteTM Mounting Medium (Biotium #23008) onto slides and imaged using Zeiss Axio Imager Z1 fluorescence microscope.
-
bioRxiv - Neuroscience 2023Quote: ... Retinae were then mounted in Drop-n-Stain EverBrite™ Mounting Medium (Biotium #23008) onto slides and imaged using Zeiss Axio Imager Z1 fluorescence microscope.
-
bioRxiv - Cell Biology 2023Quote: ... conjugated to CF555 (Mix-n-Stain CF Antibody Labeling Kit, Biotium, Fremont, CA, USA)
-
bioRxiv - Cell Biology 2020Quote: ... Cell populations deposited onto each slide were stained with GelGreen (P/N 41005; Biotium, Inc.) following manufacturer’s protocols ...
-
bioRxiv - Immunology 2020Quote: ... and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium, Fremont, CA, USA), respectively ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 µM CF647 azide (Biotium), 200 µM CuSO4 ...
-
bioRxiv - Immunology 2023Quote: ... 5 μL of MTT (Biotium) was dispensed in each well and further incubated at 37°C for 1h ...
-
bioRxiv - Microbiology 2021Quote: ... fixed with paraformaldehyde (PFA) at 4% (Biotium) and glutaraldehyde 1% (Sigma Aldrich/Merck ...
-
bioRxiv - Physiology 2023Quote: ... di-4-ANEPPS (7.5 µM, Biotium, CA) and positioned to center the anterior descending artery within the field of view ...
-
bioRxiv - Cell Biology 2020Quote: ... PCNA antibody was pre-labeled using a Mix-n-StainCF-568 Dye Antibody Labeling Kit (Biotium; 92275) accordingly to the manufacturer protocol.
-
bioRxiv - Cell Biology 2022Quote: ... primary antibodies were directly conjugated to fluorophores using Mix-n-Stain CF Dye Antibody Labeling Kits (Biotium) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: ... Dye conjugation was performed using the Mix-n-Stain CF405M Antibody Labeling Kit (Biotium, Fremont, CA USA). Anti-TERT antibody conjugated with CF405M was diluted at 1:5 in storage buffer.
-
bioRxiv - Cell Biology 2023Quote: ... primary antibodies were first labelled with fluorescent dyes using the Mix-n-Stain antibody labelling kit (Biotium) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... 5% (v/v) EvaGreen (31000, Biotium) was added to the amplification mixture to monitor the progress of library amplification ...
-
bioRxiv - Bioengineering 2022Quote: ... 4-chlorobenzenesulfonate salt) dye was purchased from Biotium and dissolved in DMSO at 10 mg/mL prior to use ...
-
bioRxiv - Neuroscience 2023Quote: ... organoids were fixed in 4%PFA (Biotium, 22023) for 3h at RT and then blocked for 1.5h at RT in 0.3% Triton-X and 10% horse serum (catalog#H0146 ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were fixed with 4% paraformaldehyde (Biotium, 22023). For lysosomal staining ...
-
bioRxiv - Cancer Biology 2020Quote: ... Ethidium Homodimer I (Biotium; 61926-22-5) per manufacturer protocol and expression of phenotypical markers was measured at day 5 via immunostaining ...
-
bioRxiv - Physiology 2021Quote: ... with or without 5 µM dipicrylamine (Biotium), using a homebuilt multi-barrel flow pipe (see above) ...
-
bioRxiv - Molecular Biology 2019Quote: ... APC-conjugated normal mouse IgG was produced using the Mix-n-Stain™ APC Antibody Labeling kit from Biotium Inc ...
-
bioRxiv - Neuroscience 2023Quote: ... CAR antibodies were conjugated with the CF647 fluorophore (CAR-647) using the Mix-n-Stain antibody labeling kit according to the manufacturer’s protocol (Biotium). Sections were processed first with rabbit polyclonal Cav1.4 or EAAT2 antibodies and corresponding secondary antibodies as described above ...