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Citations for Biotium Inc. :
101 - 150 of 185 citations for 6 Chloro 4 methyl 1H pyrrolo 3 2 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 10µL of reaction was loaded onto 2% agarose gel with GelGreen (Biotium) and ran for 30 minutes at 100V and visualized using an EZ Dock Imager (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... F(ab’)2-goat anti-mouse IgG CF568 (Biotium Cat# 20109, RRID:AB_10557119), F(ab’)2-goat anti-rabbit IgG CF568 (Biotium Cat# 20099 ...
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bioRxiv - Cell Biology 2023Quote: ... F(ab’)2-goat anti-rabbit IgG CF568 (Biotium Cat# 20099, RRID:AB_10563029), F(ab’)2-goat anti-mouse IgG Alexa 555 (Thermo Fisher Scientific Cat# A-21425 ...
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bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
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bioRxiv - Biophysics 2023Quote: ... we fixed the samples using a fixative buffer composed of 4% Paraformaldehyde (PFA, Biotium, Freemont, CA) supplemented with 2.5% of Glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2 minutes in TrueBlack Lipofuscin Autofluorescence Quencher (Biotium cat no. 23007) with 3 subsequent 1x PBS rinses before mounting and imaging.
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bioRxiv - Developmental Biology 2019Quote: ... DNA band was visualized on 2% agar gels stained with GelRed (41003, Biotium). All mammary gland tissues used in this paper are from female mice.
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Synthetic Biology 2023Quote: ... The eBlock™ integrities were individually verified by electrophoretic analysis on 3% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing was performed using PCR-mixture-2 blue (AmpliSens, Russia) and EvaGreen (Biotium, USA) as a dye ...
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bioRxiv - Bioengineering 2021Quote: ... the products were analyzed using electrophoresis on a 2% agarose gel containing GelRed (Biotium) and visualized under UV light.
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bioRxiv - Microbiology 2021Quote: PCR products were checked in 2% agarose gels stained with GelRed™ (Biotium, USA) under UV light ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions were analyzed by electrophoresis using a 2% agarose gel containing GelRed (Biotium) and visualized using an Azure c400 imager (Azure Biosystems) ...
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bioRxiv - Zoology 2022Quote: All PCR products were verified on a 2% agarose gel stained with GelRed (Biotium). From each of the 12 libraries (2*3 replicates for MiFish-U and 2*3 replicates for Mamm01 ...
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bioRxiv - Neuroscience 2023Quote: ... at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000; Biotium, CA, USA). After 90 min slices were washed in PBS (4x 10 min ...
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bioRxiv - Immunology 2023Quote: ... Samples were washed 3 times with PBS and incubated for 30 seconds with TrueBlack following the manufacturer’s instructions (Biotium, Cat# 23007). Samples were washed with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then incubated with primary antibodies in 2% BSA on a gentle shaker at room temperature for 3 days followed by three times of 1X PBS washing and incubation of secondary antibodies and CF® Phalloidin (Biotium) staining for 1 hour at room temperature in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2020Quote: ... postsynaptic nicotinic receptors (nAChRs) were labeled using α-Bungarotoxin (BTX) conjugated with CF405 (4 μg/mL; Biotium; cat ##00002) for 45 min in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Biochemistry 2023Quote: ... DNA binding experiments using plasmid-length substrates were performed under the same conditions except with 100 ng DNA per reaction and were run on 0.8% agarose gel at 4 °C and post-stained with GelRed (Biotium).
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bioRxiv - Evolutionary Biology 2020Quote: ... After electrophoresis in a 1.5% agarose gel stained with 2% GelRed (Biotium, Hayward, CA, USA), amplicons of the expected size were sequenced on both strands by Genoscreen (Lille ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were visualized on a 2% agarose gel using GelRed™ (Biotium, Freemont, CA) before multiplexing with consideration to dye color and allele size (Table S1) ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... PCR products were electrophorized on 2% agarose gels containing GelRed® (Biotium, Fremont, CA, USA) and images were captured using the ChemiDoc(tm ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were run in 2 % w/v agarose gels stained with Gel Red (Biotium).
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bioRxiv - Molecular Biology 2023Quote: ... Gels were poststained for 2 hours with polyacrylamide gel electrophoresis (PAGE) GelRed DNA stain (Biotium) and imaged using a Typhoon Imager ...
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bioRxiv - Cell Biology 2023Quote: ... All supernatants were electrophoresed on a 2% agarose gel and stained with Gel Red (Biotium).
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... the inserts were transferred to a fresh 24-well plate with black walls containing 500 μl of 4 μM Calcein AM (Biotium) in mHBSS per well ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR product was run on 2% agarose gel followed by staining with GelRed (Biotium, USA). The band size between 137nt–149nt was selected and purified using Gel Extraction Kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2021Quote: ... and qualitatively evaluated by mixing ∼2 μL of each sample with 1 μL of GelRed (Biotium), and running it on a 0.7 % agarose gel for 30 minutes at 150 V ...
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bioRxiv - Microbiology 2022Quote: ... The results of the reaction were assessed using a 2% agarose gel stained with GelRed (Biotium). Previous literature has identified this method suffers from low sensitivity ...
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bioRxiv - Microbiology 2022Quote: ... and qualitatively evaluated by mixing ~2 μL of each sample with 1 μL of GelRed (Biotium), and running it on a 0.7 % agarose gel for 30 minutes at 150 V ...
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bioRxiv - Immunology 2024Quote: ... cell viability was quantified using the ATP-Glo™ Bioluminometric Cell Viability Assay (Biotium #30020-2) on a CLARIOstar plate reader.
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bioRxiv - Biochemistry 2020Quote: ... 16 xg at 4°C and the pellet resuspended and incubated in WGA488 stain (Biotium, 5 μg/mL, diluted in HBSS) for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were incubated in a Flexstation 3 for at least 60 minutes at 37°C in Ca5 dye plus 1.25 mM probenecid (Biotium #50027, Jomar Life Research, Australia) prior to the start of recording ...
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bioRxiv - Zoology 2020Quote: ... PCR success was checked on a standard 2% agarose gel visualized with GelRed nucleic acid stain (Biotium). Sequencing of successful PCR product was performed by the Psomagen Inc ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were run on a 2% agarose gel supplemented with gel green dye (Biotium, 41005-1). A subset of F0 samples was submitted to GeneWiz for Sanger sequencing and analyzed using SnapGene software (SnapGene).