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Citations for Biotium Inc. :
1 - 50 of 119 citations for 3 Thiophen 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Cell Biology 2021Quote: ... fluo-3 acetoxymethyl (Fluo-3 thereafter) ester (Biotium, US) following a protocol adapted from (Zhang et al. ...
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bioRxiv - Microbiology 2022Quote: ... samples were placed on a microscope slide with 0.8% agarose gel containing 5 μM Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) (Biotium) and 0.75 μM permeability reporter PI (MP Biomedicals) ...
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bioRxiv - Cancer Biology 2021Quote: ... Activity of caspase-3 was detected with NucView 488 Caspase-3 Assay kit (Biotium) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... loaded with membrane-permeant AM ester forms of Fluo-3 (Fluo-3-AM) (Biotium) and/or Fura-Red ...
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bioRxiv - Cell Biology 2021Quote: ... or Proteostat (3 μM, Biotium) solutions.
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bioRxiv - Biochemistry 2024Quote: ... stained with 3 × GelRed (Biotium) for 30 min and visualized by using Chemidoc™ (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Neuroscience 2023Quote: ... Biocytin (1-3 mg/mL, Biotium) was dissolved in the pipette solution for post-hoc identification of recorded cells.
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bioRxiv - Cell Biology 2023Quote: ... loaded with Fluo-3-AM (Biotium) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) benzene sulfonic acid hydrate) Cell Viability Assay (Biotium, 30007) was performed ...
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bioRxiv - Cell Biology 2022Quote: NucView® Caspase-3 Enzyme (Biotium #10403)
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bioRxiv - Microbiology 2020Quote: ... Gels were stained with 3×GelGreen (Biotium) to visualize chromosomes.
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bioRxiv - Cancer Biology 2023Quote: ... Gel was stained with 3× Gelred (Biotium) with 0.1 M NaCl on a rocker for 30 minutes covered from light and imaged ...
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bioRxiv - Immunology 2020Quote: Cell death within tumor slices was assessed using a green-fluorescent caspase 3/7 probe that binds DNA upon cleavage by caspase 3/7 (Nucview, Biotium). 2.106 untransduced or EGFR CAR T cells were applied onto BxPC3 tumor slices that were subsequently incubated with 5μM of NucView dye ...
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bioRxiv - Plant Biology 2022Quote: ... 20 µM DiBAC4(3) (Biotium, Fermont, CA, USA) for membrane potential imaging (Fichman and Mittler ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were subsequently stained with 3× GelRed (Biotium) in distilled water for 30 minutes and imaged with a Chemidoc system (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... 5µM NucView 488 caspase-3 substrate (Biotium, 30029) and media to 50µl was added ...
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bioRxiv - Microbiology 2024Quote: ... The gels were stained with 3× GelGreen (Biotium).
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bioRxiv - Immunology 2024Quote: ... or NucView® Caspase-3 Substrate (Biotium, Fremont, CA). For staining with MitoSpy ...
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bioRxiv - Cell Biology 2023Quote: ... by IP3R-mediated ER Ca2+ release were measured in cells loaded with membrane-permeant AM ester form of Fluo-3 (Fluo-3-AM) (Biotium, Fremont, CA, USA) and Fura-Red ...
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bioRxiv - Immunology 2021Quote: ... the following reagents were used: NucView Caspase-3 enzyme substrates (Biotium), Idelalisib (Selleckchem) ...
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bioRxiv - Cancer Biology 2021Quote: The NucView Caspase-3 activity assay (Biotium, Cambridge Biosciences, Cambridge, UK) was used to confirm apoptosis observed by Hoechst 33423/PI staining in selected cell lines (RPMI 8226 and ADC-1) ...
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bioRxiv - Microbiology 2022Quote: ... NucView 488 fluorescent caspase-3/7 substrate (Biotium, Fremont, CA, USA) was added at a final concentration of 1.25µg/ml to visualize cells undergoing apoptosis ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:1000 NucView 488 Caspase-3/7 substrate (Biotium, 30029). Cells were treated with 10 µL of drug-containing media ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorescent caspase 3/7 substrate for detecting apoptosis by live staining (Biotium) at 2 µM was prepared with dosing medium (M199 +2% v/v FBS) ...
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bioRxiv - Neuroscience 2024Quote: ... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with 1 μM calcein-AM (Biotium, cat no. 80011-3) for 10 mins ...
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bioRxiv - Cell Biology 2024Quote: ... and 3 μL of 10 μg/mL biotin-4-fluorescein (Biotium, Fremont, CA) were added to the solution at a molar ratio ∼1:2-2.5 M and incubated for 30 minutes on ice.
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bioRxiv - Plant Biology 2021Quote: ... plants were fumigated with 20 μM DiBAC4(3) (Biotium, Fermont, CA; 49–52, 64), in 1.5 mM KCl buffer for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 5 μmol/L NucView™ 488 caspase-3 substrate (Biotium). Images were obtained in phase contrast and fluorescence mode using a x10 objective and an IncuCyte FLR time-lapse fluorescence microscope (Essen Bioscience) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS (3 × 5 min) and treated with Trueblack (Biotium, Cat# 23007) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... After 3 PBS washes slides were treated with 1x TrueBlack or 2x TrueBlack Plus (Biotium) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... a reagent cocktail comprised of Hoechst 33342 and NucView 488 Caspase-3 reporter (Biotium, Cat# 30029) was added to each well for final concentrations of 1 μg/mL and 1 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was analyzed using the NucView® 488 caspase-3 assay kit (Biotium, Cat# 10402-T) which contains NucView® 488 Caspase-3 substrate ...
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bioRxiv - Neuroscience 2024Quote: ... Agarose gels were prepared by boiling 2-3% agarose in TBE and adding Gel Red (41003, Biotium) at 1:10,000 concentration ...
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bioRxiv - Bioengineering 2023Quote: ... Nucleic acid was visualized with GelRed Nucleic Acid Stain (Biotium) under ultraviolet imaging and protein visualized by Coomassie Blue stain under visible light imaging.
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bioRxiv - Neuroscience 2022Quote: ... After 3 × 10 min washes in PBS slices were mounted with mounting medium with DAPI (Biotium, Fremont, CA).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
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bioRxiv - Biophysics 2024Quote: ... the gel was washed with nuclease-free water and incubated for 10-15 minutes in 3 × GelRed (Biotium) staining solution ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated with 3% H2O2 for 15 minutes and true black (catalog #: 23007 Biotium; Fremont, CA, USA) for 1 minute to reduce auto-fluorescence ...
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bioRxiv - Synthetic Biology 2023Quote: ... The eBlock™ integrities were individually verified by electrophoretic analysis on 3% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Neuroscience 2023Quote: ... at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000; Biotium, CA, USA). After 90 min slices were washed in PBS (4x 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then incubated with primary antibodies in 2% BSA on a gentle shaker at room temperature for 3 days followed by three times of 1X PBS washing and incubation of secondary antibodies and CF® Phalloidin (Biotium) staining for 1 hour at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... Samples were washed 3 times with PBS and incubated for 30 seconds with TrueBlack following the manufacturer’s instructions (Biotium, Cat# 23007). Samples were washed with PBS ...
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bioRxiv - Neuroscience 2024Quote: Neurons plated on coverslips were incubated for 1 h at RT with blocking solution of 3% BSA in PBS and incubated for ∼ 16 h at 4°C with α-bungarotoxin-488 (BGTX-488; Biotium) diluted 1/500 in 1% BSA in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... or 50 nM DNA template (mntP riboswitch, Template 6, Supplementary Table 3) with randomly distributed internal biotin-11 dNMPs (AAT BioQuest/Biotium), 0.5 µM holoRNAP ...
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...