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Citations for Biotium Inc. :
351 - 380 of 380 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The Live-or-DyeTM Zombie Aqua fixable viability staining kit was used for live cell analysis (32004-T; Biotium). For quantification ...
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bioRxiv - Immunology 2023Quote: ... Dead cells and doublets were excluded from analyses based on staining of 7-amino-actinomycin D (7-AAD) (Biotium) or Zombie yellow (catalog ...
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bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Bioengineering 2024Quote: ... the collected cells were stained with the Live-or-Dye 594/614 Fixable Viability Staining KitTM (Biotium, Fremont, CA) and then analyzed by a flow cytometer (BD LSRFortessa X20 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were subjected to various treatments for 4h and then lysed with 1X Firefly Luciferase assay buffer (Biotium Corporation). A Luciferase Assay Kit 2.0 (Biotium Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... and 48 h later luciferase activity was measured in cell lysates using the Firefly & Renilla Single Tube Luciferase Assay Kit (Biotium), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... flow cytometery was used to assess mitochondrial number and membrane potential of the cells treated with Mito Tracker (Biotium, 70075) and TMRE (tetramethylrhodamine ethyl ester ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... then maintained in complete darkness for two days prior to incubation with a cell viability stain (Live-or-Dye 488/515 (Biotium). Briefly ...
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bioRxiv - Genetics 2020Quote: To test the relative expression of XIST across cell lines quantitative real-time PCR (qPCR) was carried out using EVAgreen (Biotium) and Maxima Hot Start Taq (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The washed cells were stained with 0.1 mg/ml CF®488A wheat germ agglutinin conjugate (Biotium, Fremont, CA, USA) in PBS for 5 min at room temperature and washed two more times with PBS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then re-hydrated in PBS for 10 minutes before blocking with TrueBlack® IF blocking buffer (Biotium #23012B) for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... immediately spread as a single-cell layer between a quartz slide and coverslip and sealed with a CoverGrip sealant (Biotium, USA). Two-dimensional Raman maps were obtained with a laser excitation at 532 nm (20 mW power at the focal plane ...
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bioRxiv - Genomics 2021Quote: ... 100 µL 2x copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction buffer was added to cells to append a fluorophore reporter (2x concentrations: 10 µM CF488A picolyl azide (Biotium #92187), 2 mM CuSO4 (Sigma C8027) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: Viability of monolayers post-antibiotic treatment was assessed using the Viability/Cytotoxicity Assay for Animal Live & Dead Cells kit (Biotium, Fremont, CA) according to package insert ...
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bioRxiv - Microbiology 2024Quote: ... The bladder cells were stained for membrane (CellBrite Steady Membrane 405) and nucleus (NucSpot Live Cell 650) for 30 minutes in pre-warmed EpiLife as per manufacturer’s instructions (Biotium, San Francisco, USA). After the staining step and two washes with 1 x PBS ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed with DPBS and processed for either immunoblotting staining or were directly stained with WGA CF®488A (Biotium®) for 30 minutes at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... 1×106 cells from each liver were stained for various cell markers: Live-or-Dye™ fixable viability dye in APC-Cy™7 (Biotium, Cat ...
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bioRxiv - Neuroscience 2022Quote: ... The reporter gene construct was transiently transfected to each stable cell line and luminescence measured using Firefly Luciferase Assay Kit (Biotium, Inc., Fremont, California) and a Wallac 1420 VICTOR2 microplate reader (PerkinElmer ...
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bioRxiv - Bioengineering 2022Quote: ... Aliquots of detached cells (approximately 2×106) were incubated with a series of CF®640R succinimidyl ester-labeled (Biotium, USA, cat. 92108) ACE2 extracellular domain (AA Q18 – S740 ...
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bioRxiv - Bioengineering 2022Quote: ... the cell aggregates were processed and imaged as described in the whole-mount immunofluorescence methods using Streptavidin (29072, Biotium, 1:500, CA, USA) and a DAPI (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... by IP3R-mediated ER Ca2+ release were measured in cells loaded with membrane-permeant AM ester form of Fluo-3 (Fluo-3-AM) (Biotium, Fremont, CA, USA) and Fura-Red ...
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bioRxiv - Microbiology 2023Quote: ... and DNA from membrane-compromised bacterial cells was removed by treatment of coral homogenates and tissue airbrushed blanks with the viability dye PMAxxTM (Biotium, Fremont, CA, USA). PMAxx is a DNA-intercalating agent that forms photo-induced crosslinks making the bound DNA inaccessible for downstream molecular applications ...
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bioRxiv - Cell Biology 2022Quote: ... Cell viability was evaluated in the control condition (cells grown in the complete medium) and in the test conditions (cells grown in the hyperosmolar media) via a resazurin-based assay (Biotium, Inc., Fremont, CA, USA) performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were dried and slides were coverslipped using fluorescent mounting medium (VectaShield, Vector Laboratories, Inc., Burlingame, CA, USA; or EverBrite, Biotium, Inc, Fremont, CA USA). In some studies ...