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Citations for Biotium Inc. :
351 - 400 of 462 citations for Red colored Polystyrene Beads 1 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked for 1 hour at room temperature with 1x Fish Gelatin Blocking Agent (Biotium, #22010) in wash buffer ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was carried out using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium; 31041-1) in the QuantStudio 7 Flex Real-Time PCR System (Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... tissues were incubated with secondary antibodies coupled with fluorescent dye (dilution 1/800, Biotium, Fremont, CA, USA) according to the species in which the primary antibodies were produced for 3h at RT and with or without rhodamine-phalloidin (dilution 1/200 ...
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bioRxiv - Microbiology 2024Quote: ... CF583R-conjugated donkey anti-rabbit IgG (Custom CF Dye, lot 23C0811, Biotium, 1:250, 4 μg/mL). To confirm that the fluorophore attached to the secondary antibody does not produce artifacts ...
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bioRxiv - Microbiology 2024Quote: ... CF583R-conjugated donkey anti-mouse IgG (Custom CF Dye, lot 23C1122, Biotium, 1:250, 4 μg/mL), CF583R-conjugated donkey anti-rabbit IgG (Custom CF Dye ...
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bioRxiv - Neuroscience 2024Quote: ... Phalloidin staining of SCs in culture was performed by incubation of TexasRed Phalloidin (1:100, Biotium, 00033) for one hour at room temperature.
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bioRxiv - Bioengineering 2024Quote: Live cell staining of 3T3 cells was conducted using CellBrite Steady Dye 550 diluted 1:1000 (Biotium) for membrane staining ...
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bioRxiv - Microbiology 2020Quote: ... wells were washed twice with fresh media before CAMHB containing the eDNA stain (EthHD-2 (1 μM; Biotium)) was added to the wells ...
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bioRxiv - Genomics 2020Quote: ... Fragmented RNA was incubated in low-input Biotinylation Solution (25 mM Hepes pH 7.5, 1 mM EDTA, 25% dimethylformamide, 16.4 μM MTS-Biotin (Biotium)) for 30 minutes in the dark at 25 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... separated on a 1% agarose gel and visualized with GelRed nucleic acid stain (cat #41001, Biotium, Fremont, CA) using a Syngene G:Box Chemi imager (Syngene ...
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bioRxiv - Cell Biology 2021Quote: ... JC-1 Mitochondrial Membrane Potential Detection Kit and MitoView™ Green were purchased from Biotium (Hayward, CA, USA). Mouse monoclonal anti-α-Tubulin-FITC antibody and anti-phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in RPMI 1640 medium and stained for 5 min with 1 μg/ml of WGA-CF594 (Biotium). Gametogenesis was induced by XA at the concentration indicated for each experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed with annexin-binding buffer and additionally incubated with NucSpot Live 650 (1:1,000; Biotium) for 1 hr at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1 h at 37°C in mKRB supplemented with native coelenterazine (5 µM, BIOTIUM), and then incubated for 15 minutes in mKRB supplemented with either TPEN (500 µM ...
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bioRxiv - Synthetic Biology 2023Quote: ... Both treated cells and control cells were stained with wheat germ agglutinin (WGA) conjugates CF568 (Biotium 29077-1) to visualize the plasma membrane ...
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bioRxiv - Microbiology 2024Quote: ... and 1/1000 Alexa FluorTM 488-conjugated cross-absorbed goat anti-human IgG (heavy chain specific) (Biotium, 20444). The plates were incubated for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... the mouse brain sections were dipped briefly in TrueBlack® Lipofuscin Autofluorescence Quencher (dilution 1:10, Biotium, 23007) for 1 min ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated with goat anti-rabbit fluorescent IR dye at 680 nm (Biotium cat # 20193-1) and CF 800 goat anti-mouse secondary antibody (Biotium cat# 20831) ...
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bioRxiv - Genomics 2024Quote: ... The molecular weight of the DNA was checked on a 1% agarose gel stained with GelGreen (Biotium, BT41005), loaded with 6X purple loading dye (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... WGA and DAPI staining: Volumes of 200 µl of 1X CF®488A WGA (Biotium, REF: 29022-1) and 1X DAPI (Biotium ...
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bioRxiv - Physiology 2024Quote: ... followed by a 1-minute incubation with autofluorescence quenching reagent (TrueBlack, Cat. No. 23007; Biotium, Fremont, CA, USA) and blocked for 1 hour in 2.5% horse serum in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2024Quote: ... followed by a 1-minute incubation with autofluorescence quenching reagent (TrueBlack, Cat. No. 23007; Biotium, Fremont, CA, USA) and blocked for 1 hour with a 5% goat serum ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in secondary antibody solution which contained anti-rabbit CF488 secondary antibody (1:100; Biotium), rhodamine phalloidin (1:50 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quality control was performed by running 1 μL of eluted DNA on a 1.0% agarose gel stained with GelRed (Biotium) and visually inspecting whether bands of high molecular weight DNA were visible ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated one cochlea in HBSS containing annexin V-Alexa647 at a 1/25 dilution and CF488A-WGA (Biotium) at 20 μg/mL and the other cochlea in the same buffer supplemented with 100 μM benzamil for 25 min at RT in an orbital shaker protected from the light ...
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bioRxiv - Microbiology 2021Quote: ... The size of the amplified fragments was analyzed by 1%-agarose gel electrophoresis and DNA staining with GelRed (Biotium), and the correct insert’s nucleotide sequence was confirmed by automated DNA sequencing ...
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bioRxiv - Bioengineering 2022Quote: Frozen samples from saliva nuclease degradation assays were analysed on 1% (w/v) agarose gels stained with GelRed (Biotium) and visualised on a BioRAD Gel Doc EZ Imager with a UV transilluminator ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Cell Biology 2021Quote: ... 5 h) in 50 mM HEPES pH 7.8 containing 1 mM EDTA and stained with GelRed (Biotium, cat#41003). After washing the gel with 75 mM NaOH for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were then run on a 1% agarose gel and visualised using GelRed Nucleic Acid Gel Stain (Biotium). Only well separated bands corresponding to the main PCR products obtained were cut out from the gel and PCR products were extracted from gel slices using GenElute Agarose Spin Columns (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μg of RNA were biotinylated in biotinylation buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA, 20 ng/μl MTSEA Biotin-XX (Biotium)) for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were permeabilized with 0.5% Triton X-100 for 10 minutes and stained with CF647-phalloidin (1:40 v/v, Biotium) for 1 hour in the dark under gentle mixing ...
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bioRxiv - Cell Biology 2024Quote: ... The media was then replaced with 100µLper well supplemented F12-K medium with 500nM JF646 Halo-tag ligand and 1:1000 MembraneSteady-488nm (Biotium).
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were washed and incubated in secondary antibody solution with Goat anti-Rabbit Alexa 568 (Biotium, 1:500) and Goat anti-Mouse IgG1α Alexa 488 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Goat anti-Chat, Millipore (Cat. No. AB144P, working dilution: 1:1000) with CF555-conjugated Donkey anti-Goat IgG (Biotium, Cat ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with TrueBlack® Lipofuscin Autofluorescence Quencher (1:20 with 70% ethanol; Biotium, Fremont, CA, United States) for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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bioRxiv - Cell Biology 2024Quote: ... RNAs were biotinylated in a reaction containing 1x labeling buffer (20 mM Hepes pH 7.4, 1 mM EDTA) and 0.5 mg/ml Biotin-MTS (Biotium, #90066) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... Lectins: Peanut Germ Agglutinin conjugated with a 488 fluorophore (1:100 concentration, #29060 Biotium, San Francisco, CA, United States) and Wheat Germ Agglutinin conjugated with a 405 fluorophore (1:50 concentration ...
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bioRxiv - Cell Biology 2022Quote: ... infected MDMs were rinsed in PBS and incubated for 15 min at 37°C with a 1/1000 dilution of CellBrite Fix-640 (Biotium). Cells were rinsed twice with PBS and fixed in PFA 4% for confocal microscopy.
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA extractions were quantified with the Qubit fluorometer and quality was assessed with 1% agarose gels stained with GelRed (Biotium) and visualized with UV light.
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bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were analyzed via agarose gel electrophoresis on a 1 % (w/v) agarose gel with 0.001 % GelGreen® Nucleic Acid Gel Dye (Biotium) (90 V ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were separated by electrophoresis in a 1 % TAE-agarose gel stained with GelRed nucleic acid gel stain (Biotium), gel-purified (QIAquick gel purification Kit ...
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bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...