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Citations for Biotium Inc. :
301 - 350 of 405 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Phalloidin staining of SCs in culture was performed by incubation of TexasRed Phalloidin (1:100, Biotium, 00033) for one hour at room temperature.
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bioRxiv - Neuroscience 2022Quote: ... cells were washed twice and loaded with 25 nM TMRM and either 5 μM Nucview 488 (Biotium) or 5 μM Fluo4-AM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... they were incubated with wheat germ agglutinin (WGA) conjugated to CF©488A (5 μg/ml Biotium) at room temperature for two hours ...
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bioRxiv - Genetics 2022Quote: ... we performed PCR in 10 μl reactions using Apex Taq DNA polymerase and EvaGreen Dye (Biotium, Fremont, CA, USA) as the intercalating dye ...
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bioRxiv - Immunology 2022Quote: ... 10 μl of each sample were loaded into a 1% agarose gel with gel red (Biotium, Fremont, CA, USA) together with 2 μL 6x loading dye (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with wheat germ agglutinin (WGA) conjugated to horseradish peroxidase (HRP) (Biotium Inc. Hayward, CA, USA) at a dilution of 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA concentrations were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and diluted to 2 ng μL-1 using a Myra Liquid Handler (Bio Molecular Systems ...
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bioRxiv - Neuroscience 2023Quote: ... floating sections or tissue-mounted slides were incubated with TrueBlack® Plus Lipofuscin Autofluorescence Quencher (Biotium, Fremont, CA, USA) in 1X phosphate buffer saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: ... washed three times in 1xTBS and incubated in a 1x solution of TrueBlack lipofuscin autofluoresence quencher (Biotium, Fremont, CA) in 70% ethanol for 2 min before rinsing in 1xTBS ...
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bioRxiv - Microbiology 2023Quote: ... DNA integrity was evaluated by electrophoresis in 0.8% (w/v) agarose gels stained with GelRed (Biotium, Fremont, CA, USA).
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bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with TrueBlack® Lipofuscin Autofluorescence Quencher (1:20 with 70% ethanol; Biotium, Fremont, CA, United States) for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins ...
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bioRxiv - Bioengineering 2024Quote: ... the collected cells were stained with the Live-or-Dye 594/614 Fixable Viability Staining KitTM (Biotium, Fremont, CA) and then analyzed by a flow cytometer (BD LSRFortessa X20 ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated with 3% H2O2 for 15 minutes and true black (catalog #: 23007 Biotium; Fremont, CA, USA) for 1 minute to reduce auto-fluorescence ...
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bioRxiv - Genetics 2024Quote: ... Quantitative real-time PCR assays were performed on the Bio-Rad CFX96 instrument using Evagreen (Biotium, Freemont, CA, USA) with a procedure of 98 °C 3min ...
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bioRxiv - Neuroscience 2024Quote: ... Ha and PDa with similar densities were selected and incubated with 6 μM Fluo4-AM (Biotium, Fremont, CA, USA) in HBSS for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... the chromatin was stained with RedDot™2 Far-Red Nuclear Stain (Biotium, 1:100 diluted in PBS), and the samples mounted using Vectashield Mounting Medium and analyzed on an FV1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Readout detection probe hybridization: This is followed by hybridization of readout detection probes (100 nM) and DAPI (Biotium) in hybridization buffer for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: All experimental measurements involving electrical coupling were done in the presence of 100 nM tetrodotoxin citrate (TTX; Biotium) saline to block action potentials as well as the descending neuromodulatory inputs ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then incubated in secondary antibody solution which contained anti-rabbit CF488 secondary antibody (1:100; Biotium), rhodamine phalloidin (1:50 ...
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bioRxiv - Plant Biology 2021Quote: ... protonemal cells were stained by the 5 µM of fluorescent dye SynaptoGreen C4 (equivalent to FM1-43, Biotium, USA ...
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bioRxiv - Biochemistry 2020Quote: ... BRET signals were recorded from microsomes (each ∼10 µg) in the presence of 5 µM coelenterazine (Biotium Corp.) using the Cytation 5 image reader (BioTek Instruments) ...
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bioRxiv - Cell Biology 2022Quote: ... BRET signals were recorded from microsomes (each ∼10 μg) in the presence of 5 µM coelenterazine (Biotium Corp.) using the Cytation 5 image reader (BioTek Instruments ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was denatured at 60°C for 10 min and biotinylated using 5 µg/mL biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... samples were placed on a microscope slide with 0.8% agarose gel containing 5 μM Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) (Biotium) and 0.75 μM permeability reporter PI (MP Biomedicals) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1 h at 37°C in mKRB supplemented with native coelenterazine (5 µM, BIOTIUM), and then incubated for 15 minutes in mKRB supplemented with either TPEN (500 µM ...
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bioRxiv - Microbiology 2024Quote: 5 mg of RNA was incubated with 2.5 mg biotin-conjugated MTSEA (1 mg/ml) (Biotium 90066-1) in MTSEA buffer [20 mM HEPES pH 7.3 (Quality Biological A611-J848-10) ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in RPMI 1640 medium and stained for 5 min with 1 μg/ml of WGA-CF594 (Biotium). Gametogenesis was induced by XA at the concentration indicated for each experiment ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 45 sec at room temperature in a 1x solution of TrueBlack (Cat. #23007, Biotium, Fremont, CA) prepared in 70% ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... USA) and the products were separated through electrophoresis on a 2% agarose gel stained with GelRed (Biotium, Fremont, CA, USA). The wild-type allele generated a single fragment of 460 bp and the mutant allele generated two fragments of 276 and 184 bp.
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Microbiology 2024Quote: ... The washed cells were stained with 0.1 mg/ml CF®488A wheat germ agglutinin conjugate (Biotium, Fremont, CA, USA) in PBS for 5 min at room temperature and washed two more times with PBS buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2024Quote: ... and Wheat Germ Agglutinin conjugated with a 405 fluorophore (1:50 concentration, #29028-1 Biotium, San Francisco, CA, United States) were used to label PRC outer segments.
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bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were permeabilized with 0.5% Triton X-100 for 10 minutes and stained with CF647-phalloidin (1:40 v/v, Biotium) for 1 hour in the dark under gentle mixing ...
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bioRxiv - Genetics 2023Quote: ... The PCR products were verified using 3.5% agarose gel (in 100 ml of TAE add 3.5 g Pronadisa Agarose D1 Low EEO and 3.5 ul Gel Red Biotium Solution) and electrophoresed using Mupid-exU submarine type electrophoresis system ...
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bioRxiv - Biochemistry 2021Quote: Polymerase activity from 5°C to 50°C was measured with EvaEZ™ Fluorometric Polymerase Activity Assay Kit (Biotium) in the provided Tris buffer system based on the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 5 h) in 50 mM HEPES pH 7.8 containing 1 mM EDTA and stained with GelRed (Biotium, cat#41003). After washing the gel with 75 mM NaOH for 15 min ...
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bioRxiv - Genomics 2024Quote: Libraries were prepared using Accel NGS 2S Plus DNA library kit (IDT) following the manufacturer’s protocol with the following modification: 5% EvaGreen dye (Biotium) was added to the amplification mixture to monitor progress ...
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bioRxiv - Microbiology 2024Quote: ... qPCR was performed using 96-well plates containing 10 μL reactions composed of 5 μL of master mix (Biotium FastEvaGreen 2x ...
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bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Plant Biology 2022Quote: ... faded with 96% alcohol and processed after the addition of SynaptoRed C2 as stated by the manufacturer (Biotium, Fremont, CA, USA), following the methodology described by (55) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...