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Citations for Biotium Inc. :
251 - 300 of 369 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... followed by a 1-minute incubation with autofluorescence quenching reagent (TrueBlack, Cat. No. 23007; Biotium, Fremont, CA, USA) and blocked for 1 hour in 2.5% horse serum in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2024Quote: ... followed by a 1-minute incubation with autofluorescence quenching reagent (TrueBlack, Cat. No. 23007; Biotium, Fremont, CA, USA) and blocked for 1 hour with a 5% goat serum ...
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bioRxiv - Bioengineering 2024Quote: ... the mice were administered an intraperitoneal injection of D-luciferin (0.2 mL, 15 mg/mL; Biotium, Fremont, CA). Then ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were run in 2 % w/v agarose gels stained with Gel Red (Biotium).
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bioRxiv - Cell Biology 2023Quote: ... All supernatants were electrophoresed on a 2% agarose gel and stained with Gel Red (Biotium).
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bioRxiv - Molecular Biology 2024Quote: ... Gels were poststained for 2 hours with polyacrylamide gel electrophoresis (PAGE) GelRed DNA stain (Biotium) and imaged using a Typhoon Imager ...
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bioRxiv - Genetics 2022Quote: ... we performed PCR in 10 μl reactions using Apex Taq DNA polymerase and EvaGreen Dye (Biotium, Fremont, CA, USA) as the intercalating dye ...
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bioRxiv - Immunology 2022Quote: ... 10 μl of each sample were loaded into a 1% agarose gel with gel red (Biotium, Fremont, CA, USA) together with 2 μL 6x loading dye (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... DNA integrity was evaluated by electrophoresis in 0.8% (w/v) agarose gels stained with GelRed (Biotium, Fremont, CA, USA).
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bioRxiv - Neuroscience 2022Quote: ... washed three times in 1xTBS and incubated in a 1x solution of TrueBlack lipofuscin autofluoresence quencher (Biotium, Fremont, CA) in 70% ethanol for 2 min before rinsing in 1xTBS ...
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bioRxiv - Microbiology 2022Quote: ... the plates were incubated with wheat germ agglutinin (WGA) conjugated to horseradish peroxidase (HRP) (Biotium Inc. Hayward, CA, USA) at a dilution of 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA concentrations were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and diluted to 2 ng μL-1 using a Myra Liquid Handler (Bio Molecular Systems ...
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bioRxiv - Neuroscience 2023Quote: ... floating sections or tissue-mounted slides were incubated with TrueBlack® Plus Lipofuscin Autofluorescence Quencher (Biotium, Fremont, CA, USA) in 1X phosphate buffer saline (PBS ...
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bioRxiv - Genetics 2024Quote: ... Quantitative real-time PCR assays were performed on the Bio-Rad CFX96 instrument using Evagreen (Biotium, Freemont, CA, USA) with a procedure of 98 °C 3min ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated with 3% H2O2 for 15 minutes and true black (catalog #: 23007 Biotium; Fremont, CA, USA) for 1 minute to reduce auto-fluorescence ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with TrueBlack® Lipofuscin Autofluorescence Quencher (1:20 with 70% ethanol; Biotium, Fremont, CA, United States) for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins ...
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bioRxiv - Bioengineering 2024Quote: ... the collected cells were stained with the Live-or-Dye 594/614 Fixable Viability Staining KitTM (Biotium, Fremont, CA) and then analyzed by a flow cytometer (BD LSRFortessa X20 ...
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bioRxiv - Neuroscience 2024Quote: ... Ha and PDa with similar densities were selected and incubated with 6 μM Fluo4-AM (Biotium, Fremont, CA, USA) in HBSS for 30 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Lectins: Peanut Germ Agglutinin conjugated with a 488 fluorophore (1:100 concentration, #29060 Biotium, San Francisco, CA, United States) and Wheat Germ Agglutinin conjugated with a 405 fluorophore (1:50 concentration ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR product was run on 2% agarose gel followed by staining with GelRed (Biotium, USA). The band size between 137nt–149nt was selected and purified using Gel Extraction Kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2021Quote: ... and qualitatively evaluated by mixing ∼2 μL of each sample with 1 μL of GelRed (Biotium), and running it on a 0.7 % agarose gel for 30 minutes at 150 V ...
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bioRxiv - Immunology 2024Quote: ... cell viability was quantified using the ATP-Glo™ Bioluminometric Cell Viability Assay (Biotium #30020-2) on a CLARIOstar plate reader.
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bioRxiv - Microbiology 2022Quote: ... and qualitatively evaluated by mixing ~2 μL of each sample with 1 μL of GelRed (Biotium), and running it on a 0.7 % agarose gel for 30 minutes at 150 V ...
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bioRxiv - Microbiology 2022Quote: ... The results of the reaction were assessed using a 2% agarose gel stained with GelRed (Biotium). Previous literature has identified this method suffers from low sensitivity ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with their respective culture media + 2 µM fluo-4 AM ester (Biotium, 50018) for 15 minutes at 37 °C protected from light ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 45 sec at room temperature in a 1x solution of TrueBlack (Cat. #23007, Biotium, Fremont, CA) prepared in 70% ethanol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Microbiology 2024Quote: ... The washed cells were stained with 0.1 mg/ml CF®488A wheat germ agglutinin conjugate (Biotium, Fremont, CA, USA) in PBS for 5 min at room temperature and washed two more times with PBS buffer ...
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated at room temperature for 30 minutes in PBS containing LipidSpot reagent (1:1000; Biotium, Fremont, CA, USA), then washed 3x with PBS and mounted onto glass slides with Vectashield + DAPI.
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bioRxiv - Developmental Biology 2024Quote: ... and Wheat Germ Agglutinin conjugated with a 405 fluorophore (1:50 concentration, #29028-1 Biotium, San Francisco, CA, United States) were used to label PRC outer segments.
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bioRxiv - Zoology 2020Quote: ... PCR success was checked on a standard 2% agarose gel visualized with GelRed nucleic acid stain (Biotium). Sequencing of successful PCR product was performed by the Psomagen Inc ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were run on a 2% agarose gel supplemented with gel green dye (Biotium, 41005-1). A subset of F0 samples was submitted to GeneWiz for Sanger sequencing and analyzed using SnapGene software (SnapGene).
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bioRxiv - Neuroscience 2021Quote: Ratiometric calcium imaging experiments were conducted with the fluorescent indicator Fura-2 (Thermo Fisher Scientific or Biotium). Neurons were incubated with 5 μM Fura-2 AM and 0.04% Pluronic F-127 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Agarose gels were prepared by boiling 2-3% agarose in TBE and adding Gel Red (41003, Biotium) at 1:10,000 concentration ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a gel electrophoresis on a 2% agarose gel containing GelRed stain (Biotium, San Francisco, USA). The presence of a ∼500 bp band identified Tg2576 positive animals.
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bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Plant Biology 2022Quote: ... faded with 96% alcohol and processed after the addition of SynaptoRed C2 as stated by the manufacturer (Biotium, Fremont, CA, USA), following the methodology described by (55) ...
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bioRxiv - Neuroscience 2023Quote: ... at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000; Biotium, CA, USA). After 90 min slices were washed in PBS (4x 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were used as follows: CF 488-conjugated goat anti-mouse IgG (H+L) antibody (20302-1; Biotium, CA, USA), CF 555-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Bioengineering 2024Quote: The biocompatibility characterization of the cell-laden hydrogels was done by using a standard live/dead assay (Biotium, Fermont, CA, USA). The assay comprised fluorescent dyes Calcein-AM for staining live cells and ethidium homodimer III (EthD-III ...
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bioRxiv - Cell Biology 2021Quote: ... the chromatin was stained with RedDot™2 Far-Red Nuclear Stain (Biotium, 1:100 diluted in PBS), and the samples mounted using Vectashield Mounting Medium and analyzed on an FV1000 ...
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bioRxiv - Microbiology 2020Quote: ... wells were washed twice with fresh media before CAMHB containing the eDNA stain (EthHD-2 (1 μM; Biotium)) was added to the wells ...
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bioRxiv - Molecular Biology 2023Quote: ... purification of tachyzoites from cell debris by filtration though 3 µm filters and incubation of 2×105 extracellular parasites in 39µl of D10 with or without 30µM PMAxx (Biotium) for 15 min in shaking and darkness ...
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bioRxiv - Neuroscience 2024Quote: ... Each slice (n = 83) was injected with CF® 488A Dye Dextran (Biotium, Cat#80110; 2% solution, 10kD) using a 25 or 32 gauge Hamilton Syringe (Hamilton ...