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Citations for Biotium Inc. :
251 - 300 of 658 citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... floating sections or tissue-mounted slides were incubated with TrueBlack® Plus Lipofuscin Autofluorescence Quencher (Biotium, Fremont, CA, USA) in 1X phosphate buffer saline (PBS ...
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bioRxiv - Genetics 2024Quote: ... Quantitative real-time PCR assays were performed on the Bio-Rad CFX96 instrument using Evagreen (Biotium, Freemont, CA, USA) with a procedure of 98 °C 3min ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated with 3% H2O2 for 15 minutes and true black (catalog #: 23007 Biotium; Fremont, CA, USA) for 1 minute to reduce auto-fluorescence ...
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bioRxiv - Bioengineering 2024Quote: ... the collected cells were stained with the Live-or-Dye 594/614 Fixable Viability Staining KitTM (Biotium, Fremont, CA) and then analyzed by a flow cytometer (BD LSRFortessa X20 ...
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bioRxiv - Neuroscience 2024Quote: ... Ha and PDa with similar densities were selected and incubated with 6 μM Fluo4-AM (Biotium, Fremont, CA, USA) in HBSS for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1) and goat-anti-mouse Cy3 (1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1) and goat-anti-mouse Cy3 (1:500 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1).
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 45 sec at room temperature in a 1x solution of TrueBlack (Cat. #23007, Biotium, Fremont, CA) prepared in 70% ethanol ...
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bioRxiv - Neuroscience 2020Quote: ... USA) and the products were separated through electrophoresis on a 2% agarose gel stained with GelRed (Biotium, Fremont, CA, USA). The wild-type allele generated a single fragment of 460 bp and the mutant allele generated two fragments of 276 and 184 bp.
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Microbiology 2024Quote: ... The washed cells were stained with 0.1 mg/ml CF®488A wheat germ agglutinin conjugate (Biotium, Fremont, CA, USA) in PBS for 5 min at room temperature and washed two more times with PBS buffer ...
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Developmental Biology 2023Quote: ... we utilized fluorophore-conjugated forms of the lectin wheat germ agglutinin (WGA) (Biotium, 29022- 1, 29062-1, 29025-1) to outline all cellular membranes ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated Donkey anti-Sheep IgG (for ChAT, 1:250, #20191-1, Biotium). All antibodies were diluted in the blocking solution.
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bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Plant Biology 2022Quote: ... faded with 96% alcohol and processed after the addition of SynaptoRed C2 as stated by the manufacturer (Biotium, Fremont, CA, USA), following the methodology described by (55) ...
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bioRxiv - Bioengineering 2024Quote: The biocompatibility characterization of the cell-laden hydrogels was done by using a standard live/dead assay (Biotium, Fermont, CA, USA). The assay comprised fluorescent dyes Calcein-AM for staining live cells and ethidium homodimer III (EthD-III ...
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bioRxiv - Genomics 2021Quote: ... 1 × EvaGreen dye (Biotium) was added to the amplification mixture to monitor the PCR amplification ...
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bioRxiv - Cell Biology 2024Quote: ... + 1:1000 Enhancer (Biotium) and incubated for ∼10-15min before starting imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... Evagreen (1:20 Biotium), and 1x ROX reference dye (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 μL of aniline (diluted 1:10 in water) and 1 μL of 100 mM biocytin hydrazide (Biotium, 90060). The reaction proceeded while rotating at 4°C for 90 minutes ...
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling of protein carbonyl groups was obtained by overnight incubation of 20μg protein extracts with CF555DI hydrazide (Biotium, Fremont, CA, USA, #92165) at 4°C under medium agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were washed 3 times for 5 min with PBS to remove unbound antibody and subsequently stained with CF660C labeled anti-rabbit antibody (20183, Biotium, Fremont, CA) diluted 1:150 in PBS containing 5% (v/v ...
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bioRxiv - Genetics 2020Quote: ... PCR product was examined by 1.5% (w/v) agarose 0.5x TBE buffer (45mM Tris-borate, 1mM EDTA) gel electrophoresis with GelGreen stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Bioengineering 2020Quote: ... osteogenically induced MSCs were stained with 10 µM of the near infrared (NIR) dye CellBrite NIR680 Cytoplasmic Membrane Dye (Biotium, Fremont, CA) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were stained with 7 μM Calcofluor White for 20 minutes and 50 μg/mL of Concanavalin A (both obtained from Biotium, Fremont CA) for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GloMelt (λEx = 468 nm λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588 nm λEm = 608 nm) dyes were purchased from Biotium (Fremont, CA). The PFAS analyzed are shown in Figure 1 ...
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bioRxiv - Neuroscience 2020Quote: ... samples were passively incubated for 48 hours at room temperature in eFLASH sample buffer.32 Rapid 24 hour immunostaining of whole hemispheres with polyclonal CF640R rabbit anti-RFP (Biotium, Fremont, CA) was carried out using the eFLASH protocol.32 The samples were again passively washed in PBSN three times for a minimum of 12 hours each before refractive index matching.
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bioRxiv - Neuroscience 2023Quote: DRG neurons grown on coverslips as described above were first treated with a blocking solution consisting of sterile HBSS with 10 mM HEPES + 1% horse serum for 5 minutes at room temperature followed by staining with HBSS/HEPES + 70 mM Di-8-ANEPPS (Biotium, Fremont, CA) for 15 minutes room temperature and recovery in HBSS/HEPES + 10% horse serum for 5 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentration of DNA in the sample was further estimated using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, Fremont CA, USA) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium, Fremont, CA) in a StepOne Plus Real-Time PCR Platform (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... at the time of co-culture on day 7 followed by Live-or-Dye™ 405/545 viability stain (Biotium, Fremont, CA) on day 13 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Furimazine was purchased from Aobious (Gloucester, MA), ceolentrazine and coelentrazine analogues (coelenterazine-f, -h, -fcp, -hcp, -n, and -cp) were purchased from Biotium (Fremont, CA). Polyethyleneimine (PEI ...
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bioRxiv - Bioengineering 2024Quote: ... lungs sections were cyclically stained with antibodies in the following order: cycle 1 Rabbit RFP polyclonal antibodies (Biotium, 1:600 dilution, 1 h), cycle 2 Rabbit E-Cadherin (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... goat Anti-Guinea Pig IgG Polyclonal CF-488A (1:500, Biotium, cat#: 20017-1), cy5-AffiniPure Donkey Anti-Guinea Pig IgG H&L (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with RedDot™1 Far-Red Nuclear Stain (1:200, Biotium) at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Phalloidin CF405 (1:200; Biotium) or MitoSpy™ NIR DiIC1 dye (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Hoecsht 33342 (1:500; Biotium), and rhodamine-phalloidin (1:20 ...
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bioRxiv - Neuroscience 2022Quote: ... DAPI (1:2000; Biotium 40011), Hoechst (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EvaGreen dye (Biotium), 3 µl DNA template dilution (1×104 copies/reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EvaGreen dye (Biotium), 3 µl DNA template dilution (1×104 copies/reaction ...
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bioRxiv - Genetics 2022Quote: ... DAPI (Biotium#40043, 1:500) was used as a nuclear counterstain ...
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bioRxiv - Cell Biology 2021Quote: ... Phalloidin CF647 1:100 (Biotium) in PBS + 1% FBS ...