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Citations for Biotium Inc. :
201 - 250 of 256 citations for Anti CD117 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were Donkey anti-Goat IgG conjugated with CF488 (for GFP, 1:1000, #20016-1, Biotium), Donkey anti-Rabbit IgG conjugated with CF594 (for pERK ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated with secondary antibodies donkey anti-rat 568 (BIOTIUM, Cat. No. 20092, 1:1000), donkey anti-rabbit 647 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: The anti-GFP VHH/ Nanobody (Chomotek) was site directed conjugated with the CF680R maleimide (Biotium, Hayward, CA). The nanobody at concentration of 100uM was incubated for 2 h at room temperature with an equimolar amount of dye ...
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bioRxiv - Biochemistry 2021Quote: Polymerase activity from 5°C to 50°C was measured with EvaEZ™ Fluorometric Polymerase Activity Assay Kit (Biotium) in the provided Tris buffer system based on the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... APC-conjugated normal mouse IgG was produced using the Mix-n-Stain™ APC Antibody Labeling kit from Biotium Inc ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA concentrations were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and diluted to 2 ng μL-1 using a Myra Liquid Handler (Bio Molecular Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... The Live-or-DyeTM Zombie Aqua fixable viability staining kit was used for live cell analysis (32004-T; Biotium). For quantification ...
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bioRxiv - Molecular Biology 2023Quote: Viability of CP and CAEC exposed to hemin or vehicle was evaluated using a Viability/Cytotoxicity Assay Kit (Biotium), according to manufacturer’s guidelines ...
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bioRxiv - Genomics 2023Quote: ... The DNA samples were quantified before fragmentation reactions using Qubit and an AccuGreen Broad Range dsDNA Quantitation Kit (Biotium).
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bioRxiv - Neuroscience 2023Quote: ... CAR antibodies were conjugated with the CF647 fluorophore (CAR-647) using the Mix-n-Stain antibody labeling kit according to the manufacturer’s protocol (Biotium). Sections were processed first with rabbit polyclonal Cav1.4 or EAAT2 antibodies and corresponding secondary antibodies as described above ...
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bioRxiv - Neuroscience 2024Quote: ... cocultures exposed to TSZ after 4.5 h after were stained using a propidium iodide-based (PI) cell viability kit (Biotium) according to manufacturer protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using TUNEL assay apoptosis detection kit per the manufacturer’s instructions (Biotium). Slides were mounted in Vectashield Antifade Mounting Medium (Vector Labs) ...
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bioRxiv - Neuroscience 2020Quote: The following secondary antibodies were used in this study: CF680-conjugated donkey anti-mouse IgG antibody (Biotium, 20819), Alexa-647-conjugated donkey anti-mouse IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2024Quote: ... and 1/1000 Alexa FluorTM 488-conjugated cross-absorbed goat anti-human IgG (heavy chain specific) (Biotium, 20444). The plates were incubated for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplification buffer and enzyme mixes provided in library kit was used for the master mix together with EvaGreen for qPCR (Biotium).
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the second round PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). These libraries were then normalized ...
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... and 48 h later luciferase activity was measured in cell lysates using the Firefly & Renilla Single Tube Luciferase Assay Kit (Biotium), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Bile acids or vehicle control were added 8 hours after transfection and luciferase activity was measured 18 hours later using the luciferase assay kits (Biotium).
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were washed and incubated in secondary antibody solution with Goat anti-Rabbit Alexa 568 (Biotium, 1:500) and Goat anti-Mouse IgG1α Alexa 488 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... samples were incubated for ∼16-20 h at 37°C with donkey anti-rabbit CF568 (Biotium, catalog no. 20098) diluted 1:250 in antibody dilution buffer ...
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bioRxiv - Bioengineering 2023Quote: ... The silk products were rinsed and subsequently stained with CF555-labeled goat anti-mouse IgG (H+L) (Biotium, USA). The resultant silk products were observed using a fluorescence microscope ...
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bioRxiv - Developmental Biology 2020Quote: ... was conjugated to Alexa 647 using the Biotium Mix-n-Stain R-PE(PE) Antibody Labeling kit (Biotium, cat no: 92298) following the manufacturers protocol ...
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bioRxiv - Microbiology 2019Quote: ... with CF™ 568 dye (Mix-n-Stain™ CF® Dye Antibody Labeling Kits - CF®568, Biotium Inc, CA), and followed the nonpermeable approach for IFA [12] to investigate the interaction between Ab and ookinetes ...
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bioRxiv - Bioengineering 2022Quote: ... Following treatment cells were resuspended in 100 µL of 150 mM NaCl and stained using a viability/cytotoxicity assay kit for live and dead bacteria (Biotium, #30027) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Microbiology 2022Quote: ... NucView® 488 & MitoView™ 633 Apoptosis Assay Kit was utilized to examine cellular apoptotic state using the manufacturers protocol (Biotium). A micrograph was used to demonstrate that the Nucview488 dye (a substrate of active caspase 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-CLEC4F was from R&D (#MAB2784) and coupled to CF568 using the Mix-n-Stain CF568 Antibody Labeling Kit (Biotium, #BTM92235). All antibodies were used at 1:100 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG ...
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bioRxiv - Biophysics 2021Quote: ... Coverslips were washed three times for 5 min with PBS to remove unbound antibody and subsequently stained with CF660C labeled anti-rabbit antibody (catalog no. 20183, Biotium) or anti-mouse antibody (catalog no ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed directly in 1X RIPA buffer and blots were incubated with goat anti-mouse 680 (1:5000, cat #20253, Biotium). Fluorescent immunoreactivity was imaged on a LICOR Odyssey.
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a 1:500 dilution and detected with HRP-conjugated goat anti-mouse secondary antibody (Biotium #20400-1mL) at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Biophysics 2023Quote: ... Triton X-100 in PBS with a three-minute incubation period during each wash before being labeled with donkey anti-rabbit secondary antibodies conjugated with dye CF568 (20098-1, Biotium) at a 1:100 dilution in 1% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... The final products were purified using AMPure XP SPRI beads and quantified on a plate reader using an AccuClear DNA quantification kit (Biotium, Fremont, USA) followed by equimolar pooling ...
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Pathology 2020Quote: ... PCSK9 promoter-driven firefly luciferase activity and control renilla luciferase activity were measured using the Firefly & Renilla Luciferase Single Tube Assay Kit (#30081-1; Biotium, Fremont, CA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: Viability of monolayers post-antibiotic treatment was assessed using the Viability/Cytotoxicity Assay for Animal Live & Dead Cells kit (Biotium, Fremont, CA) according to package insert ...
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bioRxiv - Immunology 2021Quote: ... TUNEL staining was also performed with 25 μm thick floating tissue sections with the CF 640R TUNEL Assay Apoptosis Detection Kit (Biotium, Cat# 30074). Antibodies used for staining are indicated in Supplementary Table 1.
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentration of DNA in the sample was further estimated using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, Fremont CA, USA) following manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were washed three times in antibody dilution buffer and then incubated in the dark for 30 min with 1:1000 diluted secondary antibody (Goat anti-mouse AF488, Biotium, #12C0403). Cells were again washed three times before being resuspended in sort buffer (1x PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The reporter gene construct was transiently transfected to each stable cell line and luminescence measured using Firefly Luciferase Assay Kit (Biotium, Inc., Fremont, California) and a Wallac 1420 VICTOR2 microplate reader (PerkinElmer ...
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bioRxiv - Cell Biology 2022Quote: ... Mix-n-Stain CF Dye Antibody Labeling Kits (CF-680, #92282, and CF-770, #92285) were purchased from Biotium (San Francisco, CA, USA).
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bioRxiv - Immunology 2020Quote: ... Unconjugated primary antibodies CD23 and IgM were conjugated with Mix-n-Stain fluorescent protein tandem dyes antibody labeling kit for APC-CF750T (Biotium, Fremont, CA, USA) and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium ...