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Citations for Biotium Inc. :
151 - 200 of 207 citations for Streptavidin ZAP Antibody Kit rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for NOP1 ...
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bioRxiv - Neuroscience 2020Quote: ... The cover-slips were rinsed with PBSNG (3×20 min) and incubated with highly cross-absorbed donkey secondary antibodies conjugated to CF™488/594/647-Dye (1:400; Biotium, #20014 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were washed three times in antibody dilution buffer and then incubated in the dark for 30 min with 1:1000 diluted secondary antibody (Goat anti-mouse AF488, Biotium, #12C0403). Cells were again washed three times before being resuspended in sort buffer (1x PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... cells were blocked with 3% BSA in PBS for 45 minutes and then incubated with fluorophore direct-conjugated antibodies: Irf-1 conjugated with CF430 (Biotium #92118) with maleimide chemistry (Cell Signaling Technology #8478) ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then incubated with primary antibodies in 2% BSA on a gentle shaker at room temperature for 3 days followed by three times of 1X PBS washing and incubation of secondary antibodies and CF® Phalloidin (Biotium) staining for 1 hour at room temperature in the dark ...
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bioRxiv - Genomics 2021Quote: ... Final concentrations of the libraries were quantified with Accuclear ultra high sensitivity dsDNA quantification kit (Biotium) and fragment size distribution was estimated with an Agilent BioAnalyzer for a subset of 10 to 20 samples per plate ...
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bioRxiv - Cancer Biology 2021Quote: A JC-1 (5,5,6,6’-tetrachloro-1,1’,3,3’ tetraethylbenzimi-dazoylcarbocyanine iodide) mitochondrial membrane potential detection kit (Biotium, 30001) was used to measure mitochondrial membrane potential changes in live cells ...
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bioRxiv - Cancer Biology 2020Quote: Cellular death was measured with Live-or-Dye™ 488/515 Fixable Staining Kit (Biotium, USA) as per as manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: The activity of purified Taq was determined using the EvaEz fluorometric polymerase activity assay kit (Biotium). 0.4 nM Taq were used in the assay.
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Proteasomal degradation of human SERINC4: a potent host anti-HIV-1 factor that is antagonized by NefbioRxiv - Microbiology 2020Quote: ... cells were lysed and intracellular luciferase activities were determined using Firefly Luciferase Assay Kit 2.0 (Biotium). These luciferase activities were used to calculate viral infectivity.
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bioRxiv - Microbiology 2022Quote: ... Cleaned barcoded 16S rRNA amplicons were quantified using AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium) on BioTek™ FLx800™ Microplate Fluorescence Reader ...
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bioRxiv - Cell Biology 2023Quote: Free Ca2+ calibration solutions were made using Calcium Calibration Buffer Kit #1 (Cat. No. C3008MP, Biotium) according to standard protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was analyzed using the NucView® 488 caspase-3 assay kit (Biotium, Cat# 10402-T) which contains NucView® 488 Caspase-3 substrate ...
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bioRxiv - Bioengineering 2023Quote: The melting temperature of purified nanoCLAMPs was determined using GloMelt Thermal Shift Protein Stability Kit (Biotium) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Organoids were assayed for cell viability using a live/dead reagent kit (Biotium, Catalog #30002-T). Please see Sup ...
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bioRxiv - Neuroscience 2020Quote: ... washed for 10 min with 0.5% PBS-T for three times and subsequently treated with Alexa Fluor 568- or Alexa Fluor 488-conjugated fluorescent secondary antibody (1:500; Biotium, 20103, 20012) 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... Firefly and Renilla luciferase activities were measured using a Firefly & Renilla Luciferase Single Tube Assay kit (Biotium) on FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Molecular Biology 2023Quote: TUNEL assay was used to detect cell apoptosis using the CF605R TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed with PBS three times for 20 minutes and then incubated with commercial dye-conjugated secondary antibodies diluted in blocking solution (AF647-conjugates from Jackson ImmunoResearch Laboratories, West Grove, PA, and CF568-conjugates from Biotium, Fremont, CA) for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). Sequencing libraries were then pooled equally and sequenced on a NextSeq500 Mid flow cell (150 bp single-end reads).
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bioRxiv - Immunology 2021Quote: Apoptotic DNA fragmentation was examined using 30064 CF™ 594 Dye TUNEL Assay Apoptosis Detection Kit (Biotium, Cedarlane) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... JC-1 Mitochondrial Membrane Potential Detection Kit and MitoView™ Green were purchased from Biotium (Hayward, CA, USA). Mouse monoclonal anti-α-Tubulin-FITC antibody and anti-phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Genomics 2022Quote: ... Total protein level of each lane was normalized by VersaBlot™ Total Protein Normalization Kits (Biotium Cat. # 33025).
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bioRxiv - Bioengineering 2023Quote: Extracellular dsDNA release from spheroids was measured using AccuGreen™ High Sensitivity dsDNA Quantitation Kit (BIOTIUM 31066-T). Briefly ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... cells were rinsed and 1:1000 CellBrite Steady 650 membrane dye and 1:1000 kit enhancer (Biotium #30108) was added in Fluorobrite DMEM +10% FBS for 30 minutes at 37℃.
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bioRxiv - Biochemistry 2021Quote: Polymerase activity from 5°C to 50°C was measured with EvaEZ™ Fluorometric Polymerase Activity Assay Kit (Biotium) in the provided Tris buffer system based on the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA concentrations were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and diluted to 2 ng μL-1 using a Myra Liquid Handler (Bio Molecular Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... The Live-or-DyeTM Zombie Aqua fixable viability staining kit was used for live cell analysis (32004-T; Biotium). For quantification ...
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bioRxiv - Molecular Biology 2023Quote: Viability of CP and CAEC exposed to hemin or vehicle was evaluated using a Viability/Cytotoxicity Assay Kit (Biotium), according to manufacturer’s guidelines ...
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bioRxiv - Genomics 2023Quote: ... The DNA samples were quantified before fragmentation reactions using Qubit and an AccuGreen Broad Range dsDNA Quantitation Kit (Biotium).
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bioRxiv - Neuroscience 2024Quote: ... cocultures exposed to TSZ after 4.5 h after were stained using a propidium iodide-based (PI) cell viability kit (Biotium) according to manufacturer protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using TUNEL assay apoptosis detection kit per the manufacturer’s instructions (Biotium). Slides were mounted in Vectashield Antifade Mounting Medium (Vector Labs) ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplification buffer and enzyme mixes provided in library kit was used for the master mix together with EvaGreen for qPCR (Biotium).
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the second round PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). These libraries were then normalized ...
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... and 48 h later luciferase activity was measured in cell lysates using the Firefly & Renilla Single Tube Luciferase Assay Kit (Biotium), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Bile acids or vehicle control were added 8 hours after transfection and luciferase activity was measured 18 hours later using the luciferase assay kits (Biotium).
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Following treatment cells were resuspended in 100 µL of 150 mM NaCl and stained using a viability/cytotoxicity assay kit for live and dead bacteria (Biotium, #30027) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Microbiology 2022Quote: ... NucView® 488 & MitoView™ 633 Apoptosis Assay Kit was utilized to examine cellular apoptotic state using the manufacturers protocol (Biotium). A micrograph was used to demonstrate that the Nucview488 dye (a substrate of active caspase 3 ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Microbiology 2019Quote: ... The final products were purified using AMPure XP SPRI beads and quantified on a plate reader using an AccuClear DNA quantification kit (Biotium, Fremont, USA) followed by equimolar pooling ...
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...