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Citations for Biotium Inc. :
151 - 193 of 193 citations for SpectraDye Antibody Labeling Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cleaned barcoded 16S rRNA amplicons were quantified using AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium) on BioTek™ FLx800™ Microplate Fluorescence Reader ...
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bioRxiv - Cell Biology 2023Quote: Free Ca2+ calibration solutions were made using Calcium Calibration Buffer Kit #1 (Cat. No. C3008MP, Biotium) according to standard protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was analyzed using the NucView® 488 caspase-3 assay kit (Biotium, Cat# 10402-T) which contains NucView® 488 Caspase-3 substrate ...
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bioRxiv - Bioengineering 2023Quote: The melting temperature of purified nanoCLAMPs was determined using GloMelt Thermal Shift Protein Stability Kit (Biotium) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Organoids were assayed for cell viability using a live/dead reagent kit (Biotium, Catalog #30002-T). Please see Sup ...
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bioRxiv - Neuroscience 2020Quote: ... washed for 10 min with 0.5% PBS-T for three times and subsequently treated with Alexa Fluor 568- or Alexa Fluor 488-conjugated fluorescent secondary antibody (1:500; Biotium, 20103, 20012) 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... Firefly and Renilla luciferase activities were measured using a Firefly & Renilla Luciferase Single Tube Assay kit (Biotium) on FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Molecular Biology 2023Quote: TUNEL assay was used to detect cell apoptosis using the CF605R TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed with PBS three times for 20 minutes and then incubated with commercial dye-conjugated secondary antibodies diluted in blocking solution (AF647-conjugates from Jackson ImmunoResearch Laboratories, West Grove, PA, and CF568-conjugates from Biotium, Fremont, CA) for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). Sequencing libraries were then pooled equally and sequenced on a NextSeq500 Mid flow cell (150 bp single-end reads).
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bioRxiv - Immunology 2021Quote: Apoptotic DNA fragmentation was examined using 30064 CF™ 594 Dye TUNEL Assay Apoptosis Detection Kit (Biotium, Cedarlane) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... JC-1 Mitochondrial Membrane Potential Detection Kit and MitoView™ Green were purchased from Biotium (Hayward, CA, USA). Mouse monoclonal anti-α-Tubulin-FITC antibody and anti-phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Genomics 2022Quote: ... Total protein level of each lane was normalized by VersaBlot™ Total Protein Normalization Kits (Biotium Cat. # 33025).
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bioRxiv - Bioengineering 2023Quote: Extracellular dsDNA release from spheroids was measured using AccuGreen™ High Sensitivity dsDNA Quantitation Kit (BIOTIUM 31066-T). Briefly ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... cells were rinsed and 1:1000 CellBrite Steady 650 membrane dye and 1:1000 kit enhancer (Biotium #30108) was added in Fluorobrite DMEM +10% FBS for 30 minutes at 37℃.
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bioRxiv - Biochemistry 2021Quote: Polymerase activity from 5°C to 50°C was measured with EvaEZ™ Fluorometric Polymerase Activity Assay Kit (Biotium) in the provided Tris buffer system based on the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA concentrations were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and diluted to 2 ng μL-1 using a Myra Liquid Handler (Bio Molecular Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... The Live-or-DyeTM Zombie Aqua fixable viability staining kit was used for live cell analysis (32004-T; Biotium). For quantification ...
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bioRxiv - Molecular Biology 2023Quote: Viability of CP and CAEC exposed to hemin or vehicle was evaluated using a Viability/Cytotoxicity Assay Kit (Biotium), according to manufacturer’s guidelines ...
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bioRxiv - Genomics 2023Quote: ... The DNA samples were quantified before fragmentation reactions using Qubit and an AccuGreen Broad Range dsDNA Quantitation Kit (Biotium).
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bioRxiv - Neuroscience 2024Quote: ... cocultures exposed to TSZ after 4.5 h after were stained using a propidium iodide-based (PI) cell viability kit (Biotium) according to manufacturer protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplification buffer and enzyme mixes provided in library kit was used for the master mix together with EvaGreen for qPCR (Biotium).
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the second round PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). These libraries were then normalized ...
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... and 48 h later luciferase activity was measured in cell lysates using the Firefly & Renilla Single Tube Luciferase Assay Kit (Biotium), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Bile acids or vehicle control were added 8 hours after transfection and luciferase activity was measured 18 hours later using the luciferase assay kits (Biotium).
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bioRxiv - Plant Biology 2022Quote: All the PCR products were quantified with the AccuClear® Ultra High Sensitivity dsDNA quantification kit (Biotium, Fremont, CA, USA) and subpooled by sample type ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested on day 5 and the proportion of apoptotic cells was assessed using the NucView 488 Caspase-3 Assay Kit (Biotium) according to the manufacturer’s instructions and an Attune NxT flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Following treatment cells were resuspended in 100 µL of 150 mM NaCl and stained using a viability/cytotoxicity assay kit for live and dead bacteria (Biotium, #30027) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Microbiology 2022Quote: ... NucView® 488 & MitoView™ 633 Apoptosis Assay Kit was utilized to examine cellular apoptotic state using the manufacturers protocol (Biotium). A micrograph was used to demonstrate that the Nucview488 dye (a substrate of active caspase 3 ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Microbiology 2019Quote: ... The final products were purified using AMPure XP SPRI beads and quantified on a plate reader using an AccuClear DNA quantification kit (Biotium, Fremont, USA) followed by equimolar pooling ...
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Pathology 2020Quote: ... PCSK9 promoter-driven firefly luciferase activity and control renilla luciferase activity were measured using the Firefly & Renilla Luciferase Single Tube Assay Kit (#30081-1; Biotium, Fremont, CA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: Viability of monolayers post-antibiotic treatment was assessed using the Viability/Cytotoxicity Assay for Animal Live & Dead Cells kit (Biotium, Fremont, CA) according to package insert ...
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bioRxiv - Immunology 2021Quote: ... TUNEL staining was also performed with 25 μm thick floating tissue sections with the CF 640R TUNEL Assay Apoptosis Detection Kit (Biotium, Cat# 30074). Antibodies used for staining are indicated in Supplementary Table 1.
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bioRxiv - Evolutionary Biology 2023Quote: ... Concentration of DNA in the sample was further estimated using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, Fremont CA, USA) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... The reporter gene construct was transiently transfected to each stable cell line and luminescence measured using Firefly Luciferase Assay Kit (Biotium, Inc., Fremont, California) and a Wallac 1420 VICTOR2 microplate reader (PerkinElmer ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...