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Citations for Biotium Inc. :
151 - 200 of 267 citations for Mouse Caspase 2 CASP2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 6 μl of the PCR product was used for electrophoresis in Tris-Acetate EDTA running buffer at 120V for 45 min on a 2% agarose gel stained with GelRed (Biotium). Experiments were repeated twice to verify the results ...
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bioRxiv - Evolutionary Biology 2020Quote: Aliquots (2μl) of PCR products were run for 20 minutes at 80 volts in 2% agarose gels stained with GelRedTM (Biotium) and visualized in a UV light transilluminator Alpha Imager 3400 Documentation and Analysis System (Alpha Innotech ...
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bioRxiv - Neuroscience 2020Quote: ... USA) and the products were separated through electrophoresis on a 2% agarose gel stained with GelRed (Biotium, Fremont, CA, USA). The wild-type allele generated a single fragment of 460 bp and the mutant allele generated two fragments of 276 and 184 bp.
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bioRxiv - Microbiology 2021Quote: ... and the product was visualized on 2% agarose gels ran at 120V for 20 minutes and stained with GelGreen (Biotium). Primers used for probe amplification can be found in the Key Resource table..
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bioRxiv - Immunology 2021Quote: To measure apoptosis 200 µL/well NucViewTM 530 red solution (2 µM in phenol red free and serum free RMPI medium) (Biotium) was added for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were analyzed by separation of mutant and wild-type alleles on a 2% agarose gel stained with Gel Red (Biotium). Primer sequences are listed in Table S4.
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bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
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bioRxiv - Genetics 2021Quote: Success of PCR was determined by electrophoresis using a 2 % (w/v) agarose gel stained with GelRed® (Biotium, USA) and visualized under UV light ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4ul of PCR product was visualised by electrophoresis on a 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium).
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Synthetic Biology 2023Quote: ... BigBlock assemblies and PCR products were analyzed by electrophoresis on 1-2% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were analyzed on 2% agarose gel and visualized with the aid of GelGreen nucleic acid gel stain (Biotium).
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bioRxiv - Cell Biology 2024Quote: ... PCR products were identified after agarose gel electrophoresis (500 A/90 V) on a 1.5 % or 2 % agarose gel and staining with GelRed® (41003; Biotium).
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bioRxiv - Bioengineering 2024Quote: ... All samples are incubated at 37°C for 15 minutes and then run on a 2% TAE agarose gel with GelRed (Biotium). Gels were imaged at non-saturating intensities using a GeneGenius Imaging System (Syngene) ...
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bioRxiv - Cancer Biology 2020Quote: NucView 488/ MitoView 633 apoptosis assay kit (Biotium, Fremont, United States) was used to determine apoptotic cell rate according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... and the assay was performed using the Griess Reagent Kit (Biotium) following the guidelines as provided in the kit ...
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bioRxiv - Cell Biology 2022Quote: ... tendons were washed six times in PBS and placed in secondary antibody solution containing fluorescent CF488 anti-mouse secondary antibody (1:200; Biotium, Fremont, CA), Hoecsht 33342 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... at a final concentration of 20 μg/ml and incubated in the dark for 2 min before detection or with CellBrite™ Fix 488 Membrane Dye (Biotium) at a final concentration of 10X (from a 1:1000 dilution ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The larvae were housed in 10 μM INK128 (or DMSO) containing water and at 36 hpa they were treated with 5 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Biotium, #10058) for 55 min ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reaction products were separated on a 2 % agarose gel electrophoresis and visualized with GelRed (nucleic acid gel stain; #41003, Biotium, USA).
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were CF680- or CF770-conjugated anti-mouse and anti-rabbit IgG antibodies (1:5000, #20067 and #20077, Biotium, Hayward, CA, USA). Antibodies were diluted in Blocking One (Nacalai Tesque) ...
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bioRxiv - Immunology 2021Quote: ... luciferase activity was measured using a luciferase assay kit (Biotium, Hayward, CA) with a microplate reader (VictorTM X5 ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity was determined using either the Renilla Luciferase Assay Kit (Biotium), Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Bioengineering 2022Quote: Rat RVLM sections were stained using a TUNEL kit (Biotium, Fremont, CA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: The JC-1 mitochondrial membrane potential detection kit (Biotium, Hayward, CA, USA) was used for measuring mitochondrial membrane potential in oocytes ...
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bioRxiv - Bioengineering 2023Quote: ... the Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Biotium, UK) was used according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... with either AccuGreen broad-range or high-sensitivity dsDNA detection kits (Biotium). To preserve mutant abundance ratios and reduce the propagation of PCR errors ...
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bioRxiv - Physiology 2023Quote: ... preparations were loaded with Ca2+ (Rhod-2 AM, 50 µL of 1 mg/mL in DMSO + 10% pluronic acid, Biotium, Hayward, CA) and voltage (Vm)-sensitive dyes (RH237 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells stably expressing mt-AEQ were harvested at a density of 500,000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.4/NaOH) and the aequorin was reconstituted by incubation with 2 µM native coelenterazine n (Biotium, BOT-10115-1) for 2.5 h at RT ...
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bioRxiv - Bioengineering 2022Quote: ... Aliquots of detached cells (approximately 2×106) were incubated with a series of CF®640R succinimidyl ester-labeled (Biotium, USA, cat. 92108) ACE2 extracellular domain (AA Q18 – S740 ...
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bioRxiv - Synthetic Biology 2023Quote: ... All platereader experiments were performed with 20 μL volumes and the reactions were further supplemented with 2% BSA and 0.25x EvaGreen (Biotium, stock: 20x in H2O) in an optical ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...
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bioRxiv - Systems Biology 2019Quote: ... Mix-n-Stain CF555 and CF640R antibody labeling kits were purchased from Biotium Inc ...
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bioRxiv - Genomics 2021Quote: ... We measured DNA concentrations with Accuclear ultra high sensitivity dsDNA quantification kit (Biotium) and normalized all samples at a concentration of 5ng/ μL ...
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bioRxiv - Microbiology 2022Quote: ... DNA samples were quantified with AccuGreen Broad Range dsDNA quantification kit (Biotium, USA) in a Qubit Fluorometer 2.0.
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bioRxiv - Genomics 2022Quote: ... Cell-free DNA was quantified using the AccuBlue NextGen DNA Quantification Kit (Biotium).
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bioRxiv - Systems Biology 2022Quote: ... The libraries were quantified with a fluorescent assay (dsDNA AccuGreen quantitation kit, Biotium) and checked for proper size distribution and average size with a TapeStation (D1000 Tape ...
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bioRxiv - Genomics 2019Quote: ... Cells were loaded with MembriteTM Fix 488/515 cell surface staining kit (Biotium) per manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... DNA concentrations were measured with AccuBlue® Broad Range dsDNA Quantitation Kit (Biotium) DNA from mitochondrial fractions were dot blotted using Bio-Rad microfiltration apparatus onto nylon membrane in the presence of 0.4 M NaOH to denature the DNA ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the Biotium XTT Cell Viability Kit (Biotium 30007).
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bioRxiv - Cell Biology 2023Quote: ... Frozen sections were processed with TUNEL apoptosis detection kit-CF594(catalog #30064, Biotium) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Amplification products were visualized by gel electrophoresis on 2% agarose gel stained with GelRed® Nucleic Acid Gel Stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... All libraries were quantified by the AccuGreen High Sensitivity dsDNA Quantitation Kit (Biotium, USA), and the size of the libraries was determined on the Agilent Bioanalyzer 2100 (Agilent ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was quantified using Accuclear® Ultrahigh sensitivity dsDNA Quantification kit (Biotium, Fremont, USA) in a Spark® microplate reader (TECAN ...