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Citations for Biotium Inc. :
151 - 200 of 232 citations for Fab ZAP mouse Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... organs were incubated as above with Alexa Fluor 488 secondary antibody and 0.4 U/ml CF568 phalloidin (Cat#00044, Biotium). Tissue was washed three times in PBS and mounted on a glass slide in ∼50 µl of Vectashield and covered with a #1.5 thickness 22 x 22 mm cover glass (Cat#2850-22 ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were washed and incubated in secondary antibody solution with Goat anti-Rabbit Alexa 568 (Biotium, 1:500) and Goat anti-Mouse IgG1α Alexa 488 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Activity of caspase-3 was detected with NucView 488 Caspase-3 Assay kit (Biotium) according to the instructions of the manufacturer.
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bioRxiv - Molecular Biology 2021Quote: ... All libraries were quantified by the AccuGreen High Sensitivity dsDNA Quantitation Kit (Biotium, USA), and the size of the libraries was determined on the Agilent Bioanalyzer 2100 (Agilent ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was quantified using Accuclear® Ultrahigh sensitivity dsDNA Quantification kit (Biotium, Fremont, USA) in a Spark® microplate reader (TECAN ...
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bioRxiv - Biochemistry 2022Quote: Thermal melt measurements were using a GloMelt Thermal Shift protein stability kit from Biotium on a Qiagen Rotor-gene Q qPCR instrument ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were quantified using the Accuclear Ultra High Sensitivity dsDNA Quantitative kit (Biotium) and then pooled in equimolar amounts ...
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bioRxiv - Immunology 2019Quote: ... and quantified with the Accuclear Ultra High Sensitivity dsDNA kit (Biotium, Cat. No. 31028). ∼10 ng of cDNA was stamped into a fresh 384-well plate (Eppendorf ...
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bioRxiv - Bioengineering 2021Quote: ... Live-Dead assay was carried out by using Viability/Cytotoxicity Assay kit (BIOTIUM 30002). After peptide treatment ...
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bioRxiv - Plant Biology 2023Quote: ... After DNA quantification using the AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium, USA), bacterial and fungal libraries were constructed largely following our two-step PCR profiling protocol described earlier (Gfeller et al. ...
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bioRxiv - Immunology 2023Quote: ... parasite burden was measured using Steady-Luc Firefly HTS Assay Kit (Biotium, 30028-L2) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting signals were detected by incubation with secondary antibodies conjugated to fluorophores (Thermo Fisher Scientific, Biotium, or Jackson ImmunoResearch). Sections were counterstained with DAPI ...
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bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG ...
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bioRxiv - Neuroscience 2022Quote: ... samples were labeled with an anti-Myosin 7A antibody (#25-6790 Proteus Biosciences, 1:400) and goat anti-rabbit CF568 (Biotium) secondary antibody ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with an anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:400) followed by application of goat anti-rabbit CF568 (Biotium) secondary antibody ...
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bioRxiv - Bioengineering 2023Quote: ... CD81 and IgG control antibodies (Supplementary Table 1) were depleted from sodium azide using a 10kDa MWCO filter ultrafiltration vial (Biotium). Ten microgram of each antibody was conjugated overnight at 4°C with 40µg/mL NHS-AlexaFluor647 (AF647 ...
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bioRxiv - Biophysics 2023Quote: ... Triton X-100 in PBS with a three-minute incubation period during each wash before being labeled with donkey anti-rabbit secondary antibodies conjugated with dye CF568 (20098-1, Biotium) at a 1:100 dilution in 1% (w/v ...
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bioRxiv - Genomics 2023Quote: ... 5 μg of antibody in PBS (BSA-free) is mixed with 1 μl 0.33 mM CF750 Dye SE/TFP esters (Biotium 92142), Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific A20006) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by DNA quantification using the AccuGreen™ High Sensitivity dsDNA Quantitation kit (Biotium, 31066) on clear flat-bottom 96-well polystyrene plates (Corning® ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were analyzed using the Steady-Luc™ Firefly HTS Assay Kit (Biotium, Freemont, CA). Luminescence was measured using a Synergy2 plate reader (Biotek Inc ...
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bioRxiv - Immunology 2022Quote: Bacterial viability within the biofilms was evaluated using the Bacterial Viability Stain kit (Biotium®) according to the manufacturer’s description ...
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bioRxiv - Microbiology 2023Quote: ... The cells were lysed using lysis buffer supplied with Renilla Luciferase Assay Kit 2.0 (Biotium). 20 µl of cell lysate was mixed with 100 µl of freshly prepared working solution and the luminescence was recorded using a TECAN spectrophotometer.
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bioRxiv - Genomics 2024Quote: ... and the purified PCR products were quantified using the AccuBlue NextGen dsDNA Quantitation Kit (Biotium) reagents and a Spark microplate reader (Tecan Life Sciences) ...
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FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteriabioRxiv - Microbiology 2024Quote: Cells were stained with the Viability / Cytotoxicity Assay Kit for Bacteria Live & Dead Cells (Biotium). Cells were stained according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Neuroscience 2020Quote: ... The cover-slips were rinsed with PBSNG (3×20 min) and incubated with highly cross-absorbed donkey secondary antibodies conjugated to CF™488/594/647-Dye (1:400; Biotium, #20014 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then incubated with primary antibodies in 2% BSA on a gentle shaker at room temperature for 3 days followed by three times of 1X PBS washing and incubation of secondary antibodies and CF® Phalloidin (Biotium) staining for 1 hour at room temperature in the dark ...
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bioRxiv - Systems Biology 2023Quote: ... cells were blocked with 3% BSA in PBS for 45 minutes and then incubated with fluorophore direct-conjugated antibodies: Irf-1 conjugated with CF430 (Biotium #92118) with maleimide chemistry (Cell Signaling Technology #8478) ...
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bioRxiv - Genomics 2021Quote: ... Final concentrations of the libraries were quantified with Accuclear ultra high sensitivity dsDNA quantification kit (Biotium) and fragment size distribution was estimated with an Agilent BioAnalyzer for a subset of 10 to 20 samples per plate ...
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bioRxiv - Cancer Biology 2021Quote: A JC-1 (5,5,6,6’-tetrachloro-1,1’,3,3’ tetraethylbenzimi-dazoylcarbocyanine iodide) mitochondrial membrane potential detection kit (Biotium, 30001) was used to measure mitochondrial membrane potential changes in live cells ...
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bioRxiv - Cancer Biology 2020Quote: Cellular death was measured with Live-or-Dye™ 488/515 Fixable Staining Kit (Biotium, USA) as per as manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: The activity of purified Taq was determined using the EvaEz fluorometric polymerase activity assay kit (Biotium). 0.4 nM Taq were used in the assay.
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Proteasomal degradation of human SERINC4: a potent host anti-HIV-1 factor that is antagonized by NefbioRxiv - Microbiology 2020Quote: ... cells were lysed and intracellular luciferase activities were determined using Firefly Luciferase Assay Kit 2.0 (Biotium). These luciferase activities were used to calculate viral infectivity.
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bioRxiv - Microbiology 2022Quote: ... Cleaned barcoded 16S rRNA amplicons were quantified using AccuClear Ultra High Sensitivity dsDNA Quantitation Kit (Biotium) on BioTek™ FLx800™ Microplate Fluorescence Reader ...
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bioRxiv - Cell Biology 2023Quote: Free Ca2+ calibration solutions were made using Calcium Calibration Buffer Kit #1 (Cat. No. C3008MP, Biotium) according to standard protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Apoptosis was analyzed using the NucView® 488 caspase-3 assay kit (Biotium, Cat# 10402-T) which contains NucView® 488 Caspase-3 substrate ...
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bioRxiv - Bioengineering 2023Quote: The melting temperature of purified nanoCLAMPs was determined using GloMelt Thermal Shift Protein Stability Kit (Biotium) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Organoids were assayed for cell viability using a live/dead reagent kit (Biotium, Catalog #30002-T). Please see Sup ...
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bioRxiv - Neuroscience 2020Quote: ... washed for 10 min with 0.5% PBS-T for three times and subsequently treated with Alexa Fluor 568- or Alexa Fluor 488-conjugated fluorescent secondary antibody (1:500; Biotium, 20103, 20012) 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... Firefly and Renilla luciferase activities were measured using a Firefly & Renilla Luciferase Single Tube Assay kit (Biotium) on FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Molecular Biology 2023Quote: TUNEL assay was used to detect cell apoptosis using the CF605R TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed with PBS three times for 20 minutes and then incubated with commercial dye-conjugated secondary antibodies diluted in blocking solution (AF647-conjugates from Jackson ImmunoResearch Laboratories, West Grove, PA, and CF568-conjugates from Biotium, Fremont, CA) for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the concentration of the PCR products was quantified using the Accugreen High Sensitivity dsDNA Quantitation Kit (Biotium 31068). Sequencing libraries were then pooled equally and sequenced on a NextSeq500 Mid flow cell (150 bp single-end reads).
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bioRxiv - Immunology 2021Quote: Apoptotic DNA fragmentation was examined using 30064 CF™ 594 Dye TUNEL Assay Apoptosis Detection Kit (Biotium, Cedarlane) according to the manufacturer’s protocol ...