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Citations for Biotium Inc. :
151 - 173 of 173 citations for 10 Undecenamide N N bis 2 hydroxyethyl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Biophysics 2023Quote: ... the sample was then incubated for 10 min with 400 ng ml−1 WGA-CF680 (catalog no. 29029-1, Biotium) in 100 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific, Cat-No. 70-013-032; Biotium 10X Fish Gelatin Blocking Agent ...
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then re-hydrated in PBS for 10 minutes before blocking with TrueBlack® IF blocking buffer (Biotium #23012B) for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... 100 µL 2x copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction buffer was added to cells to append a fluorophore reporter (2x concentrations: 10 µM CF488A picolyl azide (Biotium #92187), 2 mM CuSO4 (Sigma C8027) ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 μL of aniline (diluted 1:10 in water) and 1 μL of 100 mM biocytin hydrazide (Biotium, 90060). The reaction proceeded while rotating at 4°C for 90 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 h at RT with the secondary antibodies HRP goat anti-mouse (1:200 in 1% BSA-PBS) followed by 10 min amplification reaction with tyramide for the detection of Ngn3 (Tyramide Amplification Kid, 33003, Biotium, USA), or Alexa 594 donkey anti-rabbit for the detection of Pomc/Npy (1:1000 in 1% BSA-PBS ...
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bioRxiv - Microbiology 2023Quote: ... All tubes were incubated in the dark at room temperature for 10 mins before being exposed to light to cross-link PMA molecules using the PMA-LiteTM LED Photolysis Device (Biotium Inc.). DNA was then isolated from all samples using either the DNeasy PowerSoil Kit (Qiagen ...
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bioRxiv - Bioengineering 2022Quote: ... Aliquots of detached cells (approximately 2×106) were incubated with a series of CF®640R succinimidyl ester-labeled (Biotium, USA, cat. 92108) ACE2 extracellular domain (AA Q18 – S740 ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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bioRxiv - Synthetic Biology 2023Quote: ... All platereader experiments were performed with 20 μL volumes and the reactions were further supplemented with 2% BSA and 0.25x EvaGreen (Biotium, stock: 20x in H2O) in an optical ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...
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bioRxiv - Bioengineering 2020Quote: ... osteogenically induced MSCs were stained with 10 µM of the near infrared (NIR) dye CellBrite NIR680 Cytoplasmic Membrane Dye (Biotium, Fremont, CA) per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: For imaging with WGA-CF680 the sample was incubated for 10 min with 1:10.000 diluted WGA-CF680 (29029-1, Biotium, Fremont, CA, USA) in 100 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Amplification products were visualized by gel electrophoresis on 2% agarose gel stained with GelRed® Nucleic Acid Gel Stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Live stain cells were washed twice using 100 µl ASW and then incubated for 10 min in 50 µg/ml WGA-CF633 conjugate (Biotium, Cat No #29024-1) in ASW ...