Labshake search
Citations for Biotium Inc. :
101 - 150 of 269 citations for Mouse NLR Family CARD Domain Containing 4 NLRC4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... samples were placed on a microscope slide with 0.8% agarose gel containing 5 μM Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) (Biotium) and 0.75 μM permeability reporter PI (MP Biomedicals) ...
-
bioRxiv - Cell Biology 2022Quote: ... CF633 donkey anti-mouse IgG (H+L) secondary antibody (Biotium #20124, 1:500).
-
bioRxiv - Cancer Biology 2024Quote: ... or donkey anti-mouse or -rabbit IgG secondary antibodies conjugated to CF568 (Biotium) or AlexaFluor647 (Jackson Immunoresearch ...
-
bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
-
bioRxiv - Biophysics 2023Quote: ... we fixed the samples using a fixative buffer composed of 4% Paraformaldehyde (PFA, Biotium, Freemont, CA) supplemented with 2.5% of Glutaraldehyde (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
-
bioRxiv - Neuroscience 2021Quote: ... we incubated one cochlea in HBSS containing annexin V-Alexa647 at a 1/25 dilution and CF488A-WGA (Biotium) at 20 μg/mL and the other cochlea in the same buffer supplemented with 100 μM benzamil for 25 min at RT in an orbital shaker protected from the light ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 h) in 50 mM HEPES pH 7.8 containing 1 mM EDTA and stained with GelRed (Biotium, cat#41003). After washing the gel with 75 mM NaOH for 15 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 11 µL H2O and run on a 1.2% agarose gel containing 1X GelGreen Nucleic Acid Stain (Biotium 41005) at 130 V for 1.5 hours ...
-
bioRxiv - Microbiology 2022Quote: ... Four μL of the ITS PCR product was mixed with 1 μL of loading dye containing Gel Red (Biotium), and visualized on a 1% agarose gel after 60 minutes at 100 V ...
-
bioRxiv - Molecular Biology 2024Quote: ... The PCR products (∼ 700 bp) were loaded into 1.5 % agarose containing GelRed Nucleic Acid Gel Stain 10,000X (Biotium®) and electrophoresed in 0.5 × TBE buffer for 1 h at 3 V/cm ...
-
bioRxiv - Neuroscience 2019Quote: ... then diluted to 6x) and run on agarose gels (1-2%) with 4% GelRed (Biotium, California, USA).
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
-
bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
-
bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
-
bioRxiv - Immunology 2021Quote: ... Second round PCR products were subjected to electrophoresis on a 1.5% agarose gel containing 0.1% Gel Red Nucleic Acid Stain (Biotium, Cat#: 41002). Positive wells were then purified using either ExoSAP-IT (Affymetrix ...
-
bioRxiv - Microbiology 2021Quote: ... cells were centrifuged to pellet (table-top centrifuge, 3000 xg, 15 min) and resuspended in PBS containing MitoView 633 (Biotium). MitoView 633 accumulates in the inner membrane as a function of membrane potential ...
-
bioRxiv - Cell Biology 2021Quote: ... All coverslips were rinsed once with 1× PBS and once with Milli-Q H2O prior to being mounted on a slide with a hardset mounting medium containing DAPI (Biotium) and allowed to cure overnight in the dark at room temperature.
-
bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
-
bioRxiv - Biophysics 2024Quote: ... live specimens were incubated for 15 − 30 min at room temperature in filtered seawater containing 1 : 1000 - 1 : 500 CellBrite Red (Biotium) lipophilic dye prior to fixation or Cell Mask Deep Red(Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: Secondary antibodies used: anti-mouse IgG (donkey, CF568-conjugated, Biotium, 20105, AB_10557030, dilution 1:500), anti-chicken IgY (goat ...
-
bioRxiv - Cancer Biology 2023Quote: ... we utilized the anti-CD44 mouse monoclonal antibody (CF488A) [clone: HCAM/918] (Biotium, BNC880918-100) with affinipure Fab fragments goat anti-mouse IgG (H+L ...
-
bioRxiv - Immunology 2022Quote: ... 100 ng of all PCR amplicons and the final dsRNA product were separated on 1% agarose gels containing 1% GelGreen (Biotium Inc.).
-
Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The larvae were housed in 10 μM INK128 (or DMSO) containing water and at 36 hpa they were treated with 5 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Biotium, #10058) for 55 min ...
-
bioRxiv - Microbiology 2020Quote: ... Infected cells were then washed with PBS buffer (pH 7.4) and resuspended in complete RPMI containing NucSpot® Live 488 (Biotium, #40081) following the manufacturer recommendations ...
-
bioRxiv - Cell Biology 2019Quote: ... The Calbryte-590 loaded cells were then incubated in HBSS buffer containing 0.5 µg/mL CF 488-tagged-Annexin-V (Biotium, cat. 29011), a fluorescence PS probe ...
-
bioRxiv - Neuroscience 2023Quote: ... Nuclei were washed twice in Wash buffer without TritonX-100 and resuspended in 100 µl of 40% ethanol containing TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, #23007) for 40-50 seconds ...
-
bioRxiv - Neuroscience 2023Quote: ... Nuclei were washed twice in Wash buffer without TritonX-100 and resuspended in 100 µl of 40% ethanol containing TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, #23007) for 40-50 seconds.
-
bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used were CF680 goat anti-mouse IgG (Biotium, cat no: 20065; dilution 1:15000) and CF770 goat anti-rabbit IgG (Biotium ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Biophysics 2019Quote: FM 4-64 (SynaptoRed™C2) and FM 2-10 (SynaptoGreen™C2) were purchased from Biotium (Fremont, CA) to use as membrane probes ...
-
bioRxiv - Neuroscience 2020Quote: ... postsynaptic nicotinic receptors (nAChRs) were labeled using α-Bungarotoxin (BTX) conjugated with CF405 (4 μg/mL; Biotium; cat ##00002) for 45 min in PBS ...
-
bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
-
bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Biochemistry 2023Quote: ... DNA binding experiments using plasmid-length substrates were performed under the same conditions except with 100 ng DNA per reaction and were run on 0.8% agarose gel at 4 °C and post-stained with GelRed (Biotium).
-
bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
-
bioRxiv - Immunology 2019Quote: ... using an AccuBlue Broad Range kit (Biotium). Then ...
-
bioRxiv - Neuroscience 2022Quote: ... Cellbrite blue (Cytoplasmic Membrane-Labeling Kit - Biotium). Craniotomy and skull surface were submerged with aCSF (see Experimental procedures ...
-
bioRxiv - Cell Biology 2019Quote: ... the Firefly Luciferase Assay Kit from Biotium was used according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: Steady-Luc HTS Assay Kit from Biotium was used for the luciferase assays ...
-
bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
-
bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... the mouse brain sections were treated with TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium, 23007, dilution 1:10) for 1 min ...
-
bioRxiv - Genetics 2024Quote: ... the mouse brain sections were treated with TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium, 23007, dilution 1:20) for 1 min ...
-
bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...