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Citations for Biotium Inc. :
101 - 150 of 227 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... protein expression of the FRHΔ100 plasmid was induced with 0.2 mM IPTG (Biotium, 10021), at 18°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... the gels were re-stained with One-Step Lumitein™ Protein Gel Stain (Biotium) as per manufacturer’s directions and imaged again as before.
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bioRxiv - Genetics 2023Quote: ... Total protein stain was then conducted by diluting CF568-labeled succinimidyl ester (Biotium, 92131) at 1:100,000 in water containing 0.1 M NaHCO3 and 25 mM Na2CO3 ...
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Synthetic Biology 2023Quote: ... The eBlock™ integrities were individually verified by electrophoretic analysis on 3% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Neuroscience 2020Quote: Secondary antibodies used: anti-mouse IgG (donkey, CF568-conjugated, Biotium, 20105, AB_10557030, dilution 1:500), anti-chicken IgY (goat ...
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bioRxiv - Cancer Biology 2023Quote: ... we utilized the anti-CD44 mouse monoclonal antibody (CF488A) [clone: HCAM/918] (Biotium, BNC880918-100) with affinipure Fab fragments goat anti-mouse IgG (H+L ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.9) and subjected to SDSCPAGE followed by Lumitein Protein Gel Stain (Biotium, Hayward, CA, USA) and immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000; Biotium, CA, USA). After 90 min slices were washed in PBS (4x 10 min ...
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bioRxiv - Immunology 2023Quote: ... Samples were washed 3 times with PBS and incubated for 30 seconds with TrueBlack following the manufacturer’s instructions (Biotium, Cat# 23007). Samples were washed with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then incubated with primary antibodies in 2% BSA on a gentle shaker at room temperature for 3 days followed by three times of 1X PBS washing and incubation of secondary antibodies and CF® Phalloidin (Biotium) staining for 1 hour at room temperature in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used were CF680 goat anti-mouse IgG (Biotium, cat no: 20065; dilution 1:15000) and CF770 goat anti-rabbit IgG (Biotium ...
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bioRxiv - Immunology 2019Quote: ... using an AccuBlue Broad Range kit (Biotium). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Cellbrite blue (Cytoplasmic Membrane-Labeling Kit - Biotium). Craniotomy and skull surface were submerged with aCSF (see Experimental procedures ...
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bioRxiv - Cell Biology 2019Quote: ... the Firefly Luciferase Assay Kit from Biotium was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Steady-Luc HTS Assay Kit from Biotium was used for the luciferase assays ...
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... the mouse brain sections were treated with TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium, 23007, dilution 1:10) for 1 min ...
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bioRxiv - Genetics 2024Quote: ... the mouse brain sections were treated with TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium, 23007, dilution 1:20) for 1 min ...
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bioRxiv - Cell Biology 2020Quote: ... antibody labeling kit Mix-n-Stain CF640R (Biotium); heme (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... or CF594 TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturers’ protocols ...
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bioRxiv - Biochemistry 2021Quote: ... Goat Anti-Rabbit (Cat. #89138-520) and anti-mouse CF680 (Cat. # 20067) secondary antibody were purchased from Biotium. Goat anti-rabbit DyLight 800 secondary antibody was purchased from Thermo Fischer Scientific (Cat ...
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bioRxiv - Neuroscience 2020Quote: The following secondary antibodies were used in this study: CF680-conjugated donkey anti-mouse IgG antibody (Biotium, 20819), Alexa-647-conjugated donkey anti-mouse IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... the mouse brain sections were dipped briefly in TrueBlack® Lipofuscin Autofluorescence Quencher (dilution 1:10, Biotium, 23007) for 1 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were incubated in a Flexstation 3 for at least 60 minutes at 37°C in Ca5 dye plus 1.25 mM probenecid (Biotium #50027, Jomar Life Research, Australia) prior to the start of recording ...
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bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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bioRxiv - Bioengineering 2023Quote: ... The silk products were rinsed and subsequently stained with CF555-labeled goat anti-mouse IgG (H+L) (Biotium, USA). The resultant silk products were observed using a fluorescence microscope ...
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bioRxiv - Molecular Biology 2023Quote: ... using the AccuGreen Broad Range dsDNA quantification kit (Biotium). Samples were diluted at the concentration of 2.5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... or Resazurin Cell Viability Assay Kit (Biotium #30025-1). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... Viafluor-488 live cell microtubule staining kit (Biotium, #70062) were used for live imaging of mNPCs in vitro.
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-goat IgG ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed directly in 1X RIPA buffer and blots were incubated with goat anti-mouse 680 (1:5000, cat #20253, Biotium). Fluorescent immunoreactivity was imaged on a LICOR Odyssey.
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG, 1:1000, Biotium; CF594-conjugated donkey anti-guinea pig IgG ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a 1:500 dilution and detected with HRP-conjugated goat anti-mouse secondary antibody (Biotium #20400-1mL) at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Cell Biology 2023Quote: ... FGOs were incubated with anti-rabbit IgG (H+L) CF 488A and anti-mouse IgG (H+L) CF 594 secondary antibodies (Biotium) (dilution 1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... and quantified using an Accuclear DNA Kit (Biotium, Fremont, USA), 75bp PE reads were generated on the Illumina HiSeq 2500.
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for Porin ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for NOP1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were washed three times in antibody dilution buffer and then incubated in the dark for 30 min with 1:1000 diluted secondary antibody (Goat anti-mouse AF488, Biotium, #12C0403). Cells were again washed three times before being resuspended in sort buffer (1x PBS ...
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bioRxiv - Cancer Biology 2020Quote: NucView 488/ MitoView 633 apoptosis assay kit (Biotium, Fremont, United States) was used to determine apoptotic cell rate according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... and the assay was performed using the Griess Reagent Kit (Biotium) following the guidelines as provided in the kit ...
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bioRxiv - Cell Biology 2022Quote: ... tendons were washed six times in PBS and placed in secondary antibody solution containing fluorescent CF488 anti-mouse secondary antibody (1:200; Biotium, Fremont, CA), Hoecsht 33342 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were CF680- or CF770-conjugated anti-mouse and anti-rabbit IgG antibodies (1:5000, #20067 and #20077, Biotium, Hayward, CA, USA). Antibodies were diluted in Blocking One (Nacalai Tesque) ...
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bioRxiv - Bioengineering 2022Quote: ... according to the manufacturer’s protocol and purified as described previously 45.The ACE2 or DnbALFA proteins were labeled by CF®640R succinimidyl ester dye (Biotium, USA, cat. 92108) in 0.1 M bicarbonate buffer using a 1:4 protein to dye ratio ...
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bioRxiv - Immunology 2021Quote: ... luciferase activity was measured using a luciferase assay kit (Biotium, Hayward, CA) with a microplate reader (VictorTM X5 ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity was determined using either the Renilla Luciferase Assay Kit (Biotium), Nano-Glo Luciferase Assay System (Promega ...