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Citations for Biotium Inc. :
101 - 144 of 144 citations for 8 Benzylthio 6 oxo octanoic Acid Methyl Ester d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... with 0.5 µM of each index primer (Oligos 2 to and 8 and 12 to 25) and 1x EvaGreen (Biotium, #31000). The protocol was 95°C for 3 min ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Amplified fragments were visualized in 2% agarose gels stained by GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA). While amplification of short fragments (~ 570 bp ...
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bioRxiv - Developmental Biology 2020Quote: ... separated on a 1% agarose gel and visualized with GelRed nucleic acid stain (cat #41001, Biotium, Fremont, CA) using a Syngene G:Box Chemi imager (Syngene ...
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bioRxiv - Microbiology 2022Quote: ... samples were placed on a microscope slide with 0.8% agarose gel containing 5 μM Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) (Biotium) and 0.75 μM permeability reporter PI (MP Biomedicals) ...
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bioRxiv - Plant Biology 2024Quote: ... based on the competition for calcium between the protein and the chromophoric chelator 5,5′-Br2-BAPTA [5,5′-dibromobis-(o-aminophenoxy)ethane N,N,N’,N’-tetra-acetic acid] (Biotium). All solutions were decalcified using Chelex-100 resin (Sigma ...
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bioRxiv - Zoology 2024Quote: ... Amplicons were visualized on 2% agarose gels stained with GelRed Nucleic Acid Gel Stain (Biotium, Hayward, CA, USA). Positive amplicons were extracted from the gels and purified using the QIAquick gel extraction kit (Qiagen ...
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bioRxiv - Pathology 2021Quote: Lipofuscin present in the brain of 6 month-old mice was quenched with TrueBlackTM Lipofuscin Autofluorescence Quencher (Biotium). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the media was changed to RPMI 1640 without phenol red supplemented with 6 μg/mL propidium iodide (Biotium). Cells were infected at an MOI of 100 for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were then run on a 1% agarose gel and visualised using GelRed Nucleic Acid Gel Stain (Biotium). Only well separated bands corresponding to the main PCR products obtained were cut out from the gel and PCR products were extracted from gel slices using GenElute Agarose Spin Columns (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and the RT-PCR products were analysed by standard agarose gel electrophoresis visualized by GelRed Nucleic Acid Stain (Biotium) and subjected to Sanger sequencing (core facility of the Haartman Institute ...
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bioRxiv - Molecular Biology 2022Quote: ... and 11 µL H2O and run on a 1.2% agarose gel containing 1X GelGreen Nucleic Acid Stain (Biotium 41005) at 130 V for 1.5 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products (∼ 700 bp) were loaded into 1.5 % agarose containing GelRed Nucleic Acid Gel Stain 10,000X (Biotium®) and electrophoresed in 0.5 × TBE buffer for 1 h at 3 V/cm ...
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bioRxiv - Neuroscience 2023Quote: DRG neurons grown on coverslips as described above were first treated with a blocking solution consisting of sterile HBSS with 10 mM HEPES + 1% horse serum for 5 minutes at room temperature followed by staining with HBSS/HEPES + 70 mM Di-8-ANEPPS (Biotium, Fremont, CA) for 15 minutes room temperature and recovery in HBSS/HEPES + 10% horse serum for 5 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Ha and PDa with similar densities were selected and incubated with 6 μM Fluo4-AM (Biotium, Fremont, CA, USA) in HBSS for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were analyzed via agarose gel electrophoresis on a 1 % (w/v) agarose gel with 0.001 % GelGreen® Nucleic Acid Gel Dye (Biotium) (90 V ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were separated by electrophoresis in a 1 % TAE-agarose gel stained with GelRed nucleic acid gel stain (Biotium), gel-purified (QIAquick gel purification Kit ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Immunology 2020Quote: ... sialic acid residues at the cell surface were oxidized using sodium meta-periodate (Thermo) and biotinylated with aminooxy-biotin (Biotium). Following quenching ...
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bioRxiv - Immunology 2021Quote: ... Second round PCR products were subjected to electrophoresis on a 1.5% agarose gel containing 0.1% Gel Red Nucleic Acid Stain (Biotium, Cat#: 41002). Positive wells were then purified using either ExoSAP-IT (Affymetrix ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4ul of PCR product was visualised by electrophoresis on a 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium).
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were analyzed on 2% agarose gel and visualized with the aid of GelGreen nucleic acid gel stain (Biotium).
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bioRxiv - Immunology 2024Quote: ... The digested plasmids were run on a 1% TAE agarose gel (with 1:20000 GelRed nucleic acid gel stain, Biotium) and the larger bands were excised for DNA purification ...
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bioRxiv - Biochemistry 2021Quote: ... The pHi of ArchT transfected RPE cells and control RPE cells were directly calculated using 2’,7’-bis(carboxyethyl)-5(6)-Carboxyfluorescein (BCECF, Biotium). BCECF is a ratiometric dual excitation/single-emission pH biosensor (405 ex/525 em for pH insensitive fluorescence ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were separated by electrophoresis on 1% agarose gel and visualized by staining with GelRed™ Nucleic Acid Gel Stain (Biotium). Nested PCR products were pooled and purified using Monofas® DNA purification Kit I (GL Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reaction products were separated on a 2 % agarose gel electrophoresis and visualized with GelRed (nucleic acid gel stain; #41003, Biotium, USA).
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bioRxiv - Plant Biology 2023Quote: ... Amplicons products were analyzed by electrophoresis on a 1% agarose gel in Tris-acetate-EDTA (TAE) buffer stained with GelRed® Nucleic Acid Gel Stain (Biotium) and visualized under UV light.
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bioRxiv - Plant Biology 2022Quote: ... PCR products were visualized by 1.5% agarose gel electrophoresis with GelRed staining (GelRed® Nucleic Acid Gel Stain, Biotium, Fremont, USA) and UV illumination ...
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bioRxiv - Genomics 2023Quote: ... PCR products were analyzed by electrophoresis on a 1% agarose gel in TAE buffer stained with GelRed® Nucleic Acid Gel Stain (Biotium) and visualized under UV light.
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bioRxiv - Bioengineering 2024Quote: ... an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) benzene sulfonic acid hydrate) Cell Viability Assay (Biotium, 30007) was performed ...
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bioRxiv - Bioengineering 2024Quote: ... Between 1-10 µL of PCR product were then run on a 2% agarose gel and nucleic acids visualized with GelRed® (Biotium). Gel documentation was performed with a BioRad GelDoc™ XR+ imager ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... or 50 nM DNA template (mntP riboswitch, Template 6, Supplementary Table 3) with randomly distributed internal biotin-11 dNMPs (AAT BioQuest/Biotium), 0.5 µM holoRNAP ...
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bioRxiv - Physiology 2023Quote: ... preparations were loaded with Ca2+ (Rhod-2 AM, 50 µL of 1 mg/mL in DMSO + 10% pluronic acid, Biotium, Hayward, CA) and voltage (Vm)-sensitive dyes (RH237 ...
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bioRxiv - Genomics 2024Quote: ... the integrity of the gDNA was verified through electrophoresis in 1% agarose gels stained with GelRed® Nucleic Acid Gel Stain (Biotium, USA), allowing visualization of high molecular weight DNA and detection of any degradation ...
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bioRxiv - Microbiology 2024Quote: The cytosolic pH of wild-type and mutant strains was measured using the pH-sensitive fluorescent dye BCECF [2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein] (Biotium; product 51011). The BCECF was added to saponin-isolated parasites by suspension (1×108 parasites/mL ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...
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bioRxiv - Bioengineering 2024Quote: ... A colorimetric assay using XTT (sodium 3’-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) was used in the form of a two-reagent kit (XTT, Biotium, Fermont, CA, USA). The working solution was prepared immediately before use as per manufacturer’s instructions by mixing 25 µL Activation Reagent with 5 mL XTT Solution ...
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bioRxiv - Microbiology 2020Quote: ... Amplification products were visualized by gel electrophoresis on 2% agarose gel stained with GelRed® Nucleic Acid Gel Stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... High-molecular weight genomic DNA was confirmed from these extractions by visualizing each isolation on a 1% agarose gel stained with GelGreen® nucleic acid stain (Biotium, Hayward, CA, USA). Isolation quantity and quality was assessed using a Qubit™ fluorometer and a NanoDrop™ One (ThermoFisher Scientific ...