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Citations for Biotium Inc. :
101 - 150 of 499 citations for 7 Bromo 2 chloromethyl 4H pyrido 1 2 a pyrimidin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... All samples are incubated at 37°C for 15 minutes and then run on a 2% TAE agarose gel with GelRed (Biotium). Gels were imaged at non-saturating intensities using a GeneGenius Imaging System (Syngene) ...
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bioRxiv - Microbiology 2020Quote: ... at a final concentration of 20 μg/ml and incubated in the dark for 2 min before detection or with CellBrite™ Fix 488 Membrane Dye (Biotium) at a final concentration of 10X (from a 1:1000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reaction products were separated on a 2 % agarose gel electrophoresis and visualized with GelRed (nucleic acid gel stain; #41003, Biotium, USA).
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... After at least 20 minutes of washout with aCSF containing 100 µM Advasep-7 (Biotium), a cyclodextrin that minimizes extraneous FM1-43 labeling 30 ...
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bioRxiv - Bioengineering 2022Quote: ... Aliquots of detached cells (approximately 2×106) were incubated with a series of CF®640R succinimidyl ester-labeled (Biotium, USA, cat. 92108) ACE2 extracellular domain (AA Q18 – S740 ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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bioRxiv - Synthetic Biology 2023Quote: ... All platereader experiments were performed with 20 μL volumes and the reactions were further supplemented with 2% BSA and 0.25x EvaGreen (Biotium, stock: 20x in H2O) in an optical ...
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bioRxiv - Synthetic Biology 2023Quote: ... the gels were re-stained with One-Step Lumitein™ Protein Gel Stain (Biotium) as per manufacturer’s directions and imaged again as before.
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bioRxiv - Microbiology 2020Quote: ... Amplification products were visualized by gel electrophoresis on 2% agarose gel stained with GelRed® Nucleic Acid Gel Stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Biophysics 2022Quote: ... The protein band was stained by One-Step Blue Protein Gel Stain (Biotium, Fremont, CA).
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bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
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bioRxiv - Microbiology 2020Quote: ... Binding was analyzed by gel electrophoresis on a 7 % polyacrylamide gel in TBE buffer stained with GelRed (Biotium) following electrophoresis.
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bioRxiv - Microbiology 2021Quote: ... fixed with paraformaldehyde (PFA) at 4% (Biotium) and glutaraldehyde 1% (Sigma Aldrich/Merck ...
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bioRxiv - Physiology 2023Quote: ... di-4-ANEPPS (7.5 µM, Biotium, CA) and positioned to center the anterior descending artery within the field of view ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Bioengineering 2022Quote: ... 4-chlorobenzenesulfonate salt) dye was purchased from Biotium and dissolved in DMSO at 10 mg/mL prior to use ...
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bioRxiv - Neuroscience 2023Quote: ... organoids were fixed in 4%PFA (Biotium, 22023) for 3h at RT and then blocked for 1.5h at RT in 0.3% Triton-X and 10% horse serum (catalog#H0146 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were fixed with 4% paraformaldehyde (Biotium, 22023). For lysosomal staining ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were blocked for one hour and were incubated with primary antibodies diluted in TrueBlack WB Antibody Diluent (Biotium) supplemented with 0.2% Tween-20 (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 µl 5x HOT FIREPol EvaGreen qPCR Supermix (Biotium), 0.5 µl forward (10 µM stock ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were stained with 7 μM Calcofluor White for 20 minutes and 50 μg/mL of Concanavalin A (both obtained from Biotium, Fremont CA) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... 1×106 cells from each liver were stained for various cell markers: Live-or-Dye™ fixable viability dye in APC-Cy™7 (Biotium, Cat ...
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bioRxiv - Bioengineering 2024Quote: ... Samples on day 7 post-inoculum were incubated with PMA before being treated with a blue LED photolysis device (Biotium, San Francisco), as described above ...
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bioRxiv - Immunology 2024Quote: ... at the time of co-culture on day 7 followed by Live-or-Dye™ 405/545 viability stain (Biotium, Fremont, CA) on day 13 ...
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bioRxiv - Microbiology 2021Quote: ... A paired sample design was used where one DNA extraction tube was treated with the chemical propidium monoazide (PMAxx Dye, 20mM in H2O from Biotium) which prevents amplification of exDNA ...
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bioRxiv - Genetics 2020Quote: ... Samples were pulse centrifuged up to 1200 rpm and split in two, where one part (n = 230, PMA treated samples) was added PMA dye (Biotium, USA to a final concentration of 50 µM ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions of each of the cell lines were separately labeled with either one or both of ViaFluor 488 SE and ViaFluor 405 SE fluorescent dyes (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The voltage-sensitive dye di-4-ANEPPS (Biotium, Catalog number #61010) was loaded into the heart via the coronary circulation ...
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bioRxiv - Microbiology 2021Quote: ... These samples were fixed with 1.2 ml of paraformaldehyde 4% (Biotium) for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% paraformaldehyde (PFA) in PBS (Biotium 22023) for 15 minutes at room temperature followed by pre-cooled (-20°C ...
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bioRxiv - Physiology 2019Quote: ... the heart was loaded with di-4-ANEPPS (Biotium, Inc., Hayward, California). A charge-coupled device (CardioCCD ...
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bioRxiv - Neuroscience 2024Quote: ... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were allowed to cure protected from light at RT for at least one day before the short edges of the coverglass only were sealed with CoverGrip (Biotium #23005, Fremont, CA, USA).
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bioRxiv - Biophysics 2022Quote: ... growth medium was replaced with 4%PFA in PBS (Biotium, 200ul per well) for 20 minutes ...
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bioRxiv - Molecular Biology 2024Quote: Cells were fixed with 4% paraformaldehyde and stained with Phalloidin-CF594 conjugate (Biotium), DAPI (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2016) using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Cell Biology 2021Quote: Staining of vacuoles: SynaptoRed™ C2 (Equivalent to FM®4-64) (Cat. # 70021, Biotium) was added to the growth media of cells (1 × 10^6 cells/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 2015) using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Genomics 2023Quote: ... 64 using 40 μl (∼40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium) in the following reaction ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated for 20 hours at 4°C with anti-IgG (CF640R, Biotium Cat# 20177 ...
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bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
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bioRxiv - Biophysics 2023Quote: ... we fixed the samples using a fixative buffer composed of 4% Paraformaldehyde (PFA, Biotium, Freemont, CA) supplemented with 2.5% of Glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... Wheat Germ Agglutinin (WGA) (1:1000, 29022-1, Biotium), and Non-muscle Myosin II A (1:100 ...