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Citations for Biotium Inc. :
101 - 150 of 530 citations for 6 Chloropyrazolo 1 5 a pyrimidine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... BigBlock assemblies and PCR products were analyzed by electrophoresis on 1-2% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2020Quote: ... Ethidium Homodimer I (Biotium; 61926-22-5) per manufacturer protocol and expression of phenotypical markers was measured at day 5 via immunostaining ...
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bioRxiv - Physiology 2021Quote: ... with or without 5 µM dipicrylamine (Biotium), using a homebuilt multi-barrel flow pipe (see above) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2021Quote: ... Samples separated on 20% PAGE gels were stained using PAGE GelRed Nucleic Acid Gel Stain (Biotium). Homemade gels using standard agarose or MetaPhor Agarose were stained using GelRed ...
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bioRxiv - Genetics 2024Quote: ... Amplification success was verified on a 0.8% agarose gel stained with GelRed Nucleic Acid Gel (Biotium). After verification ...
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bioRxiv - Microbiology 2020Quote: ... and ethidiumhomodimer-2 (Biotium, California, USA), at a concentration of 2 μM and 4 μM respectively and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2019Quote: ... RNA integrity was evaluated by electrophoresis in denaturing agarose gel (1% agarose, 5% formamide, 1X TAE) stained with 3x Gel Red (Biotium Inc., CA, USA). The RNA was quantified using the Qubit RNA Assay Kit (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then washed once with NE solution and incubated in 5 µg/mL wheat germ agglutinin conjugated to Alexa Fluor 405 (Biotium cat#29028-1) diluted in NE solution for 2.5 minutes ...
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bioRxiv - Microbiology 2019Quote: ... was incubated in biotinylation buffer (50 mM HEPES [pH 7.5], 5 mM EDTA) and 5 μg MTSEA-biotin (Biotium #90066), rotating at room temperature in the dark for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells stably expressing mt-AEQ were harvested at a density of 500,000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.4/NaOH) and the aequorin was reconstituted by incubation with 2 µM native coelenterazine n (Biotium, BOT-10115-1) for 2.5 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... DNA was separated by electrophoresis on a 0.8% agarose gel containing GelRed nucleic acid gel stain (Biotium) in Tris-acetate-EDTA buffer (120 V for 40 minutes) ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were visualized after gel electrophoresis and ultrasensitive GelRed® nucleic acid gel stain (Biotium, Fremont, CA, USA) on a UV-light transilluminator ...
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bioRxiv - Genetics 2019Quote: ... PCR amplicons were visualized via gel electrophoresis with GelRed® Nucleic Acid Gel Stain (Biotium, Fremont, California, US) and processed with the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... and DAPI (5 μg/ml) (Biotium, Cat #BT-40043). Staining with DAPI was done for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg/ml of DAPI (Biotium; Cat #BT-40043) was used as a viability dye ...
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bioRxiv - Immunology 2023Quote: ... and NucView-488 at 5 μM (Biotium, Cat. 10402). NucView-488 was selected to visualize activation of Caspase-3 in apoptosis ...
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bioRxiv - Pathology 2021Quote: Lipofuscin present in the brain of 6 month-old mice was quenched with TrueBlackTM Lipofuscin Autofluorescence Quencher (Biotium). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the media was changed to RPMI 1640 without phenol red supplemented with 6 μg/mL propidium iodide (Biotium). Cells were infected at an MOI of 100 for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... and the RT-PCR products were analysed by standard agarose gel electrophoresis visualized by GelRed Nucleic Acid Stain (Biotium) and subjected to Sanger sequencing (core facility of the Haartman Institute ...
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bioRxiv - Molecular Biology 2022Quote: ... and 11 µL H2O and run on a 1.2% agarose gel containing 1X GelGreen Nucleic Acid Stain (Biotium 41005) at 130 V for 1.5 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products (∼ 700 bp) were loaded into 1.5 % agarose containing GelRed Nucleic Acid Gel Stain 10,000X (Biotium®) and electrophoresed in 0.5 × TBE buffer for 1 h at 3 V/cm ...
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bioRxiv - Physiology 2021Quote: ... and Di-8-ANEPPS (Biotium, #61012, 2 µM) or Wheat Germ Agglutinin Alexa Fluor™-488 Conjugate (Invitrogen™ ...
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bioRxiv - Neuroscience 2022Quote: ... Pluronic F127 and Rhod-2/AM from Biotium, buprenorphine from Champion Alstoe Animal Health ...
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bioRxiv - Neuroscience 2020Quote: ... applying 5 μl of Fluorogold (Hydroxystilbamine FluoroGold, Biotium, Fremont, CA) to the area ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Immunology 2020Quote: ... sialic acid residues at the cell surface were oxidized using sodium meta-periodate (Thermo) and biotinylated with aminooxy-biotin (Biotium). Following quenching ...
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bioRxiv - Microbiology 2019Quote: ... An aliquot of the T7 transcription reaction (before and after purification) was run in a 2.0% agarose gel using GelRed® Nucleic Acid Gel Stain (Biotium) as above to confirm the production of dsRNA ...
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bioRxiv - Immunology 2021Quote: ... Second round PCR products were subjected to electrophoresis on a 1.5% agarose gel containing 0.1% Gel Red Nucleic Acid Stain (Biotium, Cat#: 41002). Positive wells were then purified using either ExoSAP-IT (Affymetrix ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Physiology 2022Quote: ... Sections were stained with RedDot 2 nuclear stain (Biotium) and WGA Oregon Green (Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... the IVT reaction was doped with 5-amino-allyl UTP (Biotium) at 1:10 (aminoallyl-UTP ...
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bioRxiv - Plant Biology 2022Quote: ... PCR products were visualized by 1.5% agarose gel electrophoresis with GelRed staining (GelRed® Nucleic Acid Gel Stain, Biotium, Fremont, USA) and UV illumination ...
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bioRxiv - Cancer Biology 2022Quote: ... using 2 μg/ml of 405-Alexa-NHS ester (Biotium).
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bioRxiv - Systems Biology 2019Quote: ... The cells were incubated with 5 µM of DCF-DA (Biotium #10058) for 30 min at 37°C MCF-10A prior to trypsinization ...
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bioRxiv - Bioengineering 2021Quote: ... stained with (5 μg/mL) CF488 Wheat Germ Agglutinin (WGA) Conjugates (Biotium), and washed 3 times with HBSS ...
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bioRxiv - Cell Biology 2022Quote: ... Permeabilized cells were incubated with 5 U/mL of fluorescent phalloidin (Biotium) in PBS for 20 min and washed with PBS.
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bioRxiv - Neuroscience 2023Quote: ... Cholera toxin subunit B (ctb)- 640 or -568 (5 mg/ml, Biotium) was injected in NAc medial shell and core (from bregma ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µl of ready to use nDiO solution ‘Cellbrite™ Green’ (Biotium) was added to 200 µl purified virus and incubated for 1 h on a rotation wheel at RT ...
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bioRxiv - Microbiology 2022Quote: ... Resulting reactions were run on 2% agarose gel with GelGreen (Biotium) before imaging using an EZ Dock Imager (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: The free radical probe 2’,7’-dichlorodihydrofluoresceindiacetate (H2DCFDA) (Biotium, Fremont, CA) was used to quantify ROS ...
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bioRxiv - Physiology 2023Quote: ... followed by donkey anti-rat CF750 (2 µg/mL, 20857, Biotium). Slides were imaged with the Slideview VS200 (Olympus ...
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bioRxiv - Genomics 2024Quote: ... DNA was loaded onto 2% agarose gel including 1X GelRed (Biotium).
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bioRxiv - Neuroscience 2024Quote: ... all sections were treated with TrueBlack lipofuscin autofluorescence quencher (5%; Biotium cat: 23007) for 90 seconds followed by a 3x5 minute rinse in 1M PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 5 μmol/L NucView™ 488 caspase-3 substrate (Biotium). Images were obtained in phase contrast and fluorescence mode using a x10 objective and an IncuCyte FLR time-lapse fluorescence microscope (Essen Bioscience) ...