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Citations for Biotium Inc. :
51 - 100 of 142 citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Following treatment cells were resuspended in 100 µL of 150 mM NaCl and stained using a viability/cytotoxicity assay kit for live and dead bacteria (Biotium, #30027) according to the manufacturers protocol ...
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bioRxiv - Microbiology 2022Quote: ... NucView® 488 & MitoView™ 633 Apoptosis Assay Kit was utilized to examine cellular apoptotic state using the manufacturers protocol (Biotium). A micrograph was used to demonstrate that the Nucview488 dye (a substrate of active caspase 3 ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Genetics 2022Quote: ... and mounted with Drop-n-Stain EverBrite Mounting Medium with DAPI (BIOTIUM: 23009). All specimens were imaged using Zeiss Axio Imager.M1 fluorescence microscope with a 63X plan-apo Chromat oil objective ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were then mounted in Drop-n-Stain EverBriteTM Mounting Medium (Biotium #23008) onto slides and imaged using Zeiss Axio Imager Z1 fluorescence microscope.
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bioRxiv - Microbiology 2022Quote: ... disodium salt (XTT) colorimetric assay (Biotium) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Pathology 2020Quote: ... PCSK9 promoter-driven firefly luciferase activity and control renilla luciferase activity were measured using the Firefly & Renilla Luciferase Single Tube Assay Kit (#30081-1; Biotium, Fremont, CA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: Viability of monolayers post-antibiotic treatment was assessed using the Viability/Cytotoxicity Assay for Animal Live & Dead Cells kit (Biotium, Fremont, CA) according to package insert ...
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bioRxiv - Immunology 2021Quote: ... TUNEL staining was also performed with 25 μm thick floating tissue sections with the CF 640R TUNEL Assay Apoptosis Detection Kit (Biotium, Cat# 30074). Antibodies used for staining are indicated in Supplementary Table 1.
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bioRxiv - Neuroscience 2020Quote: Intracellular chloride measurements were performed using the N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide (MQAE) (Biotium) chloride sensor.
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bioRxiv - Neuroscience 2023Quote: ... Retinae were then mounted in Drop-n-Stain EverBrite™ Mounting Medium (Biotium #23008) onto slides and imaged using Zeiss Axio Imager Z1 fluorescence microscope.
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bioRxiv - Neuroscience 2022Quote: ... The reporter gene construct was transiently transfected to each stable cell line and luminescence measured using Firefly Luciferase Assay Kit (Biotium, Inc., Fremont, California) and a Wallac 1420 VICTOR2 microplate reader (PerkinElmer ...
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bioRxiv - Cancer Biology 2021Quote: ... The CF™ 488 TUNEL Assay (Biotium) was performed according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... Cell populations deposited onto each slide were stained with GelGreen (P/N 41005; Biotium, Inc.) following manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... we used an XTT assay (Biotium, Cat #30007). Using either 15% FBS or 0.5% FBS media ...
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bioRxiv - Genetics 2020Quote: ... Samples were pulse centrifuged up to 1200 rpm and split in two, where one part (n = 230, PMA treated samples) was added PMA dye (Biotium, USA to a final concentration of 50 µM ...
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bioRxiv - Cancer Biology 2021Quote: The NucView Caspase-3 activity assay (Biotium, Cambridge Biosciences, Cambridge, UK) was used to confirm apoptosis observed by Hoechst 33423/PI staining in selected cell lines (RPMI 8226 and ADC-1) ...
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bioRxiv - Bioengineering 2021Quote: ... Assays read using intercalating dyes received 1X EvaGreen (Biotium, Freemont, CA, USA) instead of OSD probes ...
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bioRxiv - Synthetic Biology 2019Quote: ... The final three wells were prepared with Luciferase Assay Buffer (Biotium #30085). Plates were scanned in a microplate reader (Biotek Synergy H1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... qPCR assays were run with Forget-Me-Not Master Mix (Biotium 31044) in an Eppendorf Realplex 2 or BioRad CFX96 machine ...
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bioRxiv - Bioengineering 2024Quote: ... the samples were immersed in live/dead assay (Biotium, Hayward, CA, USA) solution for 30 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Furimazine was purchased from Aobious (Gloucester, MA), ceolentrazine and coelentrazine analogues (coelenterazine-f, -h, -fcp, -hcp, -n, and -cp) were purchased from Biotium (Fremont, CA). Polyethyleneimine (PEI ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells stably expressing mt-AEQ were harvested at a density of 500,000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.4/NaOH) and the aequorin was reconstituted by incubation with 2 µM native coelenterazine n (Biotium, BOT-10115-1) for 2.5 h at RT ...
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bioRxiv - Genomics 2021Quote: ... DNA concentrations were confirmed using AccuBlue Broad Range assay (Biotium, Inc., Fremont, USA), followed by normalization and DNA library construction ...
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bioRxiv - Biophysics 2019Quote: Differential Scanning Fluorimetry based thermal shift assays were conducted using GloMelt reagents (Biotium) according to manufacturer’s protocol with slight modifications ...
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bioRxiv - Bioengineering 2022Quote: Viability of encapsulated cells was measured by staining with Live/Dead viability assays (Biotium, USA), a cellular membrane integrity assay that stains live cells with green fluorescence and dead cells with red fluorescence ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed after 5 days by XTT Cell Proliferation Assay (Biotium, Fremont, CA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... cell viability was quantified using the ATP-Glo™ Bioluminometric Cell Viability Assay (Biotium #30020-2) on a CLARIOstar plate reader.
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Bioengineering 2022Quote: Frozen samples from saliva nuclease degradation assays were analysed on 1% (w/v) agarose gels stained with GelRed (Biotium) and visualised on a BioRAD Gel Doc EZ Imager with a UV transilluminator ...
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bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... The PCR product’s concentration was quantified with Qubit (dsDNA High Sensitivity Assay) and its length was verified on a 2.5% agarose gel with GelRed (Biotium). Excess primers and dNTPs were removed by ExoSAP-IT (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product’s concentration was quantified with Qubit (dsDNA High Sensitivity or Broad Range Assay) and its length was verified on a 2.5% agarose gel with GelRed (Biotium). Excess primers and dNTPs were removed by ExoSAP-IT (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real-time PCR assays were performed on the Bio-Rad CFX96 instrument using Evagreen (Biotium, Freemont, CA, USA) with a procedure of 98 °C 3min ...
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
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bioRxiv - Immunology 2019Quote: ... using an AccuBlue Broad Range kit (Biotium). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Cellbrite blue (Cytoplasmic Membrane-Labeling Kit - Biotium). Craniotomy and skull surface were submerged with aCSF (see Experimental procedures ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... These cross-sectioned samples were washed twice with PBS to remove the culture media before being incubated in a live/dead assay staining solution (obtained from Biotium, Hayward, CA, USA) for a duration of 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and APCs seeded on plastic (2D control) were assessed using fluorescent calcein AM/ethidium homodimer III (EtDHIII) Live/Dead assay (30002-T, Biotium Inc, Insight Biotech, UK) and Click-iT® EdU (5-ethynyl-2’deoxyuridine ...
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bioRxiv - Cell Biology 2022Quote: ... Cell viability was evaluated in the control condition (cells grown in the complete medium) and in the test conditions (cells grown in the hyperosmolar media) via a resazurin-based assay (Biotium, Inc., Fremont, CA, USA) performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Real-time PCR reactions were then performed to determine the mRNA levels of target genes or DNA levels using RT2 Forget-Me-NotTM EvaGreen qPCR Mastermix (Biotium, Assay Matrix, Melbourne, Australia) on a CFX Connect Real Time PCR System (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... using the AccuGreen Broad Range dsDNA quantification kit (Biotium). Samples were diluted at the concentration of 2.5 ng/μl ...
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bioRxiv - Neuroscience 2024Quote: ... Viafluor-488 live cell microtubule staining kit (Biotium, #70062) were used for live imaging of mNPCs in vitro.
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bioRxiv - Microbiology 2019Quote: ... and quantified using an Accuclear DNA Kit (Biotium, Fremont, USA), 75bp PE reads were generated on the Illumina HiSeq 2500.
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bioRxiv - Bioengineering 2022Quote: Rat RVLM sections were stained using a TUNEL kit (Biotium, Fremont, CA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2021Quote: The JC-1 mitochondrial membrane potential detection kit (Biotium, Hayward, CA, USA) was used for measuring mitochondrial membrane potential in oocytes ...