Labshake search
Citations for Biotium Inc. :
51 - 80 of 80 citations for 7 8H Pteridinone 4 methylamino 7CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... we fixed the samples using a fixative buffer composed of 4% Paraformaldehyde (PFA, Biotium, Freemont, CA) supplemented with 2.5% of Glutaraldehyde (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
-
bioRxiv - Neuroscience 2019Quote: ... then diluted to 6x) and run on agarose gels (1-2%) with 4% GelRed (Biotium, California, USA).
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
-
bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
-
bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Biophysics 2019Quote: FM 4-64 (SynaptoRed™C2) and FM 2-10 (SynaptoGreen™C2) were purchased from Biotium (Fremont, CA) to use as membrane probes ...
-
bioRxiv - Neuroscience 2020Quote: ... postsynaptic nicotinic receptors (nAChRs) were labeled using α-Bungarotoxin (BTX) conjugated with CF405 (4 μg/mL; Biotium; cat ##00002) for 45 min in PBS ...
-
bioRxiv - Systems Biology 2021Quote: Genomic DNA was quantified using Sybr Green fluorescence assay with a 6-point DNA standard curve (0, 0.5, 1, 2, 4, 6 ng/μL Biotium). 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 μl of the digested DNA was mixed with 2 μl 1:100 in DMSO diluted GelRed™ (Biotium) dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
-
bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Biochemistry 2023Quote: ... DNA binding experiments using plasmid-length substrates were performed under the same conditions except with 100 ng DNA per reaction and were run on 0.8% agarose gel at 4 °C and post-stained with GelRed (Biotium).
-
bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
-
bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
-
bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
-
bioRxiv - Immunology 2021Quote: ... the inserts were transferred to a fresh 24-well plate with black walls containing 500 μl of 4 μM Calcein AM (Biotium) in mHBSS per well ...
-
bioRxiv - Biochemistry 2020Quote: ... 16 xg at 4°C and the pellet resuspended and incubated in WGA488 stain (Biotium, 5 μg/mL, diluted in HBSS) for 30 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were then washed with PHEM-T and counter-stained with 4′,6-diamidino-2-phenylindole (DAPI, Biotium, Fremont, CA). The cells and coverslips were mounted on glass slides with Prolong Glass Antifade Mountant (Thermo Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
-
bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... multi-shank neural probe (NeuroNexus A4×4-3mm-100-125-177-A16, Ann Arbor, MI, USA) pre-coated with DiI (Biotium, Fremont, CA, USA) for fluorescent detection of recording sites was advanced dorsoventrally at 5 µm/s through the IC using a hydraulic micropositioner (Model 650 ...