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Citations for Biotium Inc. :
601 - 650 of 662 citations for 7 tert butoxycarbonyl 5 6 7 8 tetrahydro 1 2 4 triazolo 4 3 a pyrazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated with goat anti-rabbit fluorescent IR dye at 680 nm (Biotium cat # 20193-1) and CF 800 goat anti-mouse secondary antibody (Biotium cat# 20831) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quality control was performed by running 1 μL of eluted DNA on a 1.0% agarose gel stained with GelRed (Biotium) and visually inspecting whether bands of high molecular weight DNA were visible ...
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bioRxiv - Neuroscience 2021Quote: ... we incubated one cochlea in HBSS containing annexin V-Alexa647 at a 1/25 dilution and CF488A-WGA (Biotium) at 20 μg/mL and the other cochlea in the same buffer supplemented with 100 μM benzamil for 25 min at RT in an orbital shaker protected from the light ...
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bioRxiv - Microbiology 2021Quote: ... The size of the amplified fragments was analyzed by 1%-agarose gel electrophoresis and DNA staining with GelRed (Biotium), and the correct insert’s nucleotide sequence was confirmed by automated DNA sequencing ...
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bioRxiv - Bioengineering 2022Quote: Frozen samples from saliva nuclease degradation assays were analysed on 1% (w/v) agarose gels stained with GelRed (Biotium) and visualised on a BioRAD Gel Doc EZ Imager with a UV transilluminator ...
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bioRxiv - Immunology 2022Quote: ... 10 μl of each sample were loaded into a 1% agarose gel with gel red (Biotium, Fremont, CA, USA) together with 2 μL 6x loading dye (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μg of RNA were biotinylated in biotinylation buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA, 20 ng/μl MTSEA Biotin-XX (Biotium)) for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were washed and incubated in secondary antibody solution with Goat anti-Rabbit Alexa 568 (Biotium, 1:500) and Goat anti-Mouse IgG1α Alexa 488 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Four μL of the ITS PCR product was mixed with 1 μL of loading dye containing Gel Red (Biotium), and visualized on a 1% agarose gel after 60 minutes at 100 V ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were permeabilized with 0.5% Triton X-100 for 10 minutes and stained with CF647-phalloidin (1:40 v/v, Biotium) for 1 hour in the dark under gentle mixing ...
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bioRxiv - Cell Biology 2024Quote: ... The media was then replaced with 100µLper well supplemented F12-K medium with 500nM JF646 Halo-tag ligand and 1:1000 MembraneSteady-488nm (Biotium).
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bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with TrueBlack® Lipofuscin Autofluorescence Quencher (1:20 with 70% ethanol; Biotium, Fremont, CA, United States) for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins ...
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bioRxiv - Cell Biology 2022Quote: ... infected MDMs were rinsed in PBS and incubated for 15 min at 37°C with a 1/1000 dilution of CellBrite Fix-640 (Biotium). Cells were rinsed twice with PBS and fixed in PFA 4% for confocal microscopy.
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA extractions were quantified with the Qubit fluorometer and quality was assessed with 1% agarose gels stained with GelRed (Biotium) and visualized with UV light.
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bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of bacteria suspension was added to 250 μL of enhancer for Gram-negative Bacteria (Biotium San Francisco, US). PMAxx™ Dye was dissolved in H2O at 5mM and added to a bacterial solution at a final concentration of 25μM ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG ...
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bioRxiv - Zoology 2022Quote: ... Endosymbiont presence was detected by separation of PCR products on a 1% agarose gel stained with GelRed® (Biotium, Germany), and reactions were visualised under UV light ...
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bioRxiv - Cell Biology 2019Quote: ... freshly thawed cells were diluted at 1×106 cells/mL and CellBrite TM NIR790 Cytoplasmic Membrane Dye (Biotium, cat.30079) was added at the concentration of 1µM and incubated at 37°C for 20 minutes with frequent agitation ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed directly in 1X RIPA buffer and blots were incubated with goat anti-mouse 680 (1:5000, cat #20253, Biotium). Fluorescent immunoreactivity was imaged on a LICOR Odyssey.
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bioRxiv - Microbiology 2022Quote: ... 20 μL of bacterial cultures were incubated with Wheat Germ Agglutinin Alexa Fluor 488 Conjugate (Biotium; Catalogue no. 29022–1) at a final concentration of 0.1 mg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... samples were labeled with an anti-Myosin 7A antibody (#25-6790 Proteus Biosciences, 1:400) and goat anti-rabbit CF568 (Biotium) secondary antibody ...
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bioRxiv - Genomics 2023Quote: ... 5 μg of antibody in PBS (BSA-free) is mixed with 1 μl 0.33 mM CF750 Dye SE/TFP esters (Biotium 92142), Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific A20006) ...
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with an anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:400) followed by application of goat anti-rabbit CF568 (Biotium) secondary antibody ...
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bioRxiv - Plant Biology 2023Quote: ... The amplified products were run on a 1% agarose gel (Duchefa, Netherlands) stained with GelRed® (Biotium, San Francisco, USA) and visualized under ultraviolet light.
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
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bioRxiv - Developmental Biology 2023Quote: ... to obtain single-cell suspensions.68,86 Cells were stained with a far-red cell membrane-permeant nuclear dye (RedDot™1, Biotium) to specifically select live cells and DAPI to exclude dead cells ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a 1:500 dilution and detected with HRP-conjugated goat anti-mouse secondary antibody (Biotium #20400-1mL) at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Bioengineering 2023Quote: ... CD81 and IgG control antibodies (Supplementary Table 1) were depleted from sodium azide using a 10kDa MWCO filter ultrafiltration vial (Biotium). Ten microgram of each antibody was conjugated overnight at 4°C with 40µg/mL NHS-AlexaFluor647 (AF647 ...
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bioRxiv - Biophysics 2023Quote: ... Triton X-100 in PBS with a three-minute incubation period during each wash before being labeled with donkey anti-rabbit secondary antibodies conjugated with dye CF568 (20098-1, Biotium) at a 1:100 dilution in 1% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... 1x106 cells/ml cells were stained with Live-or-Dye™ fixable dead cell dyes (1:1,000 dilution, cat#32016, Biotium) at RT for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated at room temperature for 30 minutes in PBS containing LipidSpot reagent (1:1000; Biotium, Fremont, CA, USA), then washed 3x with PBS and mounted onto glass slides with Vectashield + DAPI.
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bioRxiv - Molecular Biology 2024Quote: 10 mcg total RNA (extracted without exposure to DTT) was biotinylated by mixing with 1 mcg MTSEA-biotin-XX (Biotium) in 10 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then left to cool down at room temperature and subsequently incubated for 1 minute in TrueBlack lipofuscin autofluorescence quencher (Biotium, USA) diluted 1:20 in 70% ethanol ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Developmental Biology 2021Quote: ... centrifuged for 10 min at ∼13,000 x g and then 25uL of the clear supernatant lysate was used separately in firefly (Biotium #30085–1) and renilla (Biotium 300821 ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for Porin ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for NOP1 ...
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bioRxiv - Neuroscience 2020Quote: ... The cover-slips were rinsed with PBSNG (3×20 min) and incubated with highly cross-absorbed donkey secondary antibodies conjugated to CF™488/594/647-Dye (1:400; Biotium, #20014 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were washed three times in antibody dilution buffer and then incubated in the dark for 30 min with 1:1000 diluted secondary antibody (Goat anti-mouse AF488, Biotium, #12C0403). Cells were again washed three times before being resuspended in sort buffer (1x PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... centrifuged for 10 min at ∼13,000×g and then 50 µl of the clear supernatant lysate was used separately in firefly (Biotium #30085-1) and renilla (Biotium 300821 ...
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bioRxiv - Systems Biology 2023Quote: ... cells were blocked with 3% BSA in PBS for 45 minutes and then incubated with fluorophore direct-conjugated antibodies: Irf-1 conjugated with CF430 (Biotium #92118) with maleimide chemistry (Cell Signaling Technology #8478) ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were used as follows: CF 488-conjugated goat anti-mouse IgG (H+L) antibody (20302-1; Biotium, CA, USA), CF 555-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... On day 10-11 ITS media was removed and fresh ITS media containing a 1:2500 dilution of Lysoview-405 (Biotium; 70066) was added ...
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bioRxiv - Microbiology 2021Quote: ... The resultant triplicate PCR products for each sample were pooled and quantified using a 1% agarose gel stained with GelRed (Biotium, CA, USA). Quantified pooled triplicates were then combined in equimolar concentrations based on gel quantification to create an amplicon library ...