Labshake search
Citations for Biotium Inc. :
501 - 550 of 559 citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2021Quote: ... DNA extractions were quantified with the Qubit fluorometer and quality was assessed with 1% agarose gels stained with GelRed (Biotium) and visualized with UV light.
-
bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
-
bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
-
bioRxiv - Microbiology 2022Quote: ... 1 mL of bacteria suspension was added to 250 μL of enhancer for Gram-negative Bacteria (Biotium San Francisco, US). PMAxx™ Dye was dissolved in H2O at 5mM and added to a bacterial solution at a final concentration of 25μM ...
-
bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS for 5 minutes and incubated for 1 minute in TrueBlack lipofuscin autofluorescence quencher (Biotium #23007) in 70% ethanol ...
-
bioRxiv - Zoology 2022Quote: ... Endosymbiont presence was detected by separation of PCR products on a 1% agarose gel stained with GelRed® (Biotium, Germany), and reactions were visualised under UV light ...
-
bioRxiv - Genomics 2021Quote: ... cells were lysed directly in 1X RIPA buffer and blots were incubated with goat anti-mouse 680 (1:5000, cat #20253, Biotium). Fluorescent immunoreactivity was imaged on a LICOR Odyssey.
-
bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with an anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:400) followed by application of goat anti-rabbit CF568 (Biotium) secondary antibody ...
-
bioRxiv - Plant Biology 2023Quote: ... The amplified products were run on a 1% agarose gel (Duchefa, Netherlands) stained with GelRed® (Biotium, San Francisco, USA) and visualized under ultraviolet light.
-
bioRxiv - Synthetic Biology 2023Quote: ... BigBlock assemblies and PCR products were analyzed by electrophoresis on 1-2% agarose-TBE gels stained with GelRed® (Biotium).
-
Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS for 5 min and incubated for 1 min in TrueBlack lipofuscin autofluorescence quencher (Biotium #23,007) in 70% ethanol ...
-
bioRxiv - Developmental Biology 2023Quote: ... to obtain single-cell suspensions.68,86 Cells were stained with a far-red cell membrane-permeant nuclear dye (RedDot™1, Biotium) to specifically select live cells and DAPI to exclude dead cells ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were labeled with an anti-Myosin 7A antibody (#25-6790 Proteus Biosciences, 1:400) and goat anti-rabbit CF568 (Biotium) secondary antibody ...
-
bioRxiv - Bioengineering 2023Quote: ... CD81 and IgG control antibodies (Supplementary Table 1) were depleted from sodium azide using a 10kDa MWCO filter ultrafiltration vial (Biotium). Ten microgram of each antibody was conjugated overnight at 4°C with 40µg/mL NHS-AlexaFluor647 (AF647 ...
-
bioRxiv - Microbiology 2022Quote: ... 20 μL of bacterial cultures were incubated with Wheat Germ Agglutinin Alexa Fluor 488 Conjugate (Biotium; Catalogue no. 29022–1) at a final concentration of 0.1 mg/mL ...
-
bioRxiv - Cell Biology 2023Quote: ... was used at a 1:500 dilution and detected with HRP-conjugated goat anti-mouse secondary antibody (Biotium #20400-1mL) at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
-
bioRxiv - Cancer Biology 2024Quote: ... 1x106 cells/ml cells were stained with Live-or-Dye™ fixable dead cell dyes (1:1,000 dilution, cat#32016, Biotium) at RT for 30 min ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were incubated at room temperature for 30 minutes in PBS containing LipidSpot reagent (1:1000; Biotium, Fremont, CA, USA), then washed 3x with PBS and mounted onto glass slides with Vectashield + DAPI.
-
bioRxiv - Neuroscience 2024Quote: ... before 2h incubation at RT with the secondary antibodies (diluted at 1:500): donkey anti-goat CF-647 (Biotium 20048) and donkey anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific 21206) ...
-
bioRxiv - Molecular Biology 2024Quote: 10 mcg total RNA (extracted without exposure to DTT) was biotinylated by mixing with 1 mcg MTSEA-biotin-XX (Biotium) in 10 mM HEPES ...
-
bioRxiv - Microbiology 2024Quote: ... All extracted DNA samples were qualitatively evaluated by mixing ∼2 μL of each sample with 1 μL of loading dye containing Gel Red (Biotium) and running it on a 0.7 % agarose gel for 50 minutes at 110 V ...
-
bioRxiv - Neuroscience 2020Quote: ... The sections were then left to cool down at room temperature and subsequently incubated for 1 minute in TrueBlack lipofuscin autofluorescence quencher (Biotium, USA) diluted 1:20 in 70% ethanol ...
-
bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
-
bioRxiv - Developmental Biology 2021Quote: ... centrifuged for 10 min at ∼13,000 x g and then 25uL of the clear supernatant lysate was used separately in firefly (Biotium #30085–1) and renilla (Biotium 300821 ...
-
bioRxiv - Neuroscience 2020Quote: ... The cover-slips were rinsed with PBSNG (3×20 min) and incubated with highly cross-absorbed donkey secondary antibodies conjugated to CF™488/594/647-Dye (1:400; Biotium, #20014 ...
-
bioRxiv - Developmental Biology 2023Quote: ... centrifuged for 10 min at ∼13,000×g and then 50 µl of the clear supernatant lysate was used separately in firefly (Biotium #30085-1) and renilla (Biotium 300821 ...
-
bioRxiv - Systems Biology 2023Quote: ... cells were blocked with 3% BSA in PBS for 45 minutes and then incubated with fluorophore direct-conjugated antibodies: Irf-1 conjugated with CF430 (Biotium #92118) with maleimide chemistry (Cell Signaling Technology #8478) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Cells were washed three times in antibody dilution buffer and then incubated in the dark for 30 min with 1:1000 diluted secondary antibody (Goat anti-mouse AF488, Biotium, #12C0403). Cells were again washed three times before being resuspended in sort buffer (1x PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies were used as follows: CF 488-conjugated goat anti-mouse IgG (H+L) antibody (20302-1; Biotium, CA, USA), CF 555-conjugated goat anti-mouse IgG (H+L ...
-
bioRxiv - Cell Biology 2024Quote: ... On day 10-11 ITS media was removed and fresh ITS media containing a 1:2500 dilution of Lysoview-405 (Biotium; 70066) was added ...
-
bioRxiv - Neuroscience 2024Quote: ... tissue containing the APC was isolated in cold PBS and transferred to a solution with MitoView Green (1:500 in PBS; Biotium 70054) for 30 min before fixing with 4% PFA ...
-
bioRxiv - Immunology 2024Quote: ... urothelial sheets were stained with fluorescent dyes NucSpot650 and CellBrite405 (final concentration 1:100 in PBS as per manufacturers’ recommendations, Biotium, US) for 40 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... The sections were washed in 1X PBS and then covered with True Black (diluted at 1:20 in 70% Ethanol; Biotium; 23014), a blocker of lipofuscin ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were washed with 1X PBS and then covered with True Black (diluted at 1:20 in 70% Ethanol; Biotium; 23014), a blocker of lipofuscin ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... for 15 minutes and incubated in secondary 546 Alexa Fluor anti-mouse 1:1000 (Fisher A11003) and phalloidin 647 (Biotium 00041) 1:80 for 2 hours in the dark at room temperature in BSA/PBS ...
-
bioRxiv - Microbiology 2021Quote: ... The resultant triplicate PCR products for each sample were pooled and quantified using a 1% agarose gel stained with GelRed (Biotium, CA, USA). Quantified pooled triplicates were then combined in equimolar concentrations based on gel quantification to create an amplicon library ...
-
bioRxiv - Cell Biology 2022Quote: ... tendons were washed six times in PBS and placed in secondary antibody solution containing fluorescent CF488 anti-mouse secondary antibody (1:200; Biotium, Fremont, CA), Hoecsht 33342 (1:500 ...
-
bioRxiv - Neuroscience 2020Quote: ... washed for 10 min with 0.5% PBS-T for three times and subsequently treated with Alexa Fluor 568- or Alexa Fluor 488-conjugated fluorescent secondary antibody (1:500; Biotium, 20103, 20012) 1 hr at room temperature ...
-
bioRxiv - Pathology 2020Quote: ... PCSK9 promoter-driven firefly luciferase activity and control renilla luciferase activity were measured using the Firefly & Renilla Luciferase Single Tube Assay Kit (#30081-1; Biotium, Fremont, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... 1 mL of each fecal inoculum was mixed with 2.5 µL of 20 mM propidium monoazide (PMA) dye (Biotium, Fremont, CA, USA). The samples were then incubated in the dark for 5 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
-
bioRxiv - Bioengineering 2022Quote: ... the samples were washed three times with DPBS, and the solution of To-pro3 (Thermo Fischer Scientific, T3605, 1:1000) or Hoechst (Biotium, 33342, 5μM) was added and incubated for 30 min at RT in the dark to stain the nuclei ...
-
bioRxiv - Cell Biology 2024Quote: ... HeLa cells stably expressing mt-AEQ were harvested at a density of 500,000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.4/NaOH) and the aequorin was reconstituted by incubation with 2 µM native coelenterazine n (Biotium, BOT-10115-1) for 2.5 h at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... GloMelt (λEx = 468, λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588, λEm = 608 nm) dyes (CAT #33022-1) were purchased from Biotium (Fremont, CA). Bovine serum albumin (BSA ...
-
bioRxiv - Neuroscience 2024Quote: ... Lipofuscin autofluorescence was quenched by incubating the slices in 2x TrueBlack Plus (Biotium, diluted from stock at 1:20 in 1x PBS) for 10 minutes ...