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Citations for VWR :
101 - 135 of 135 citations for ssc mir 432 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Each 20 μL PCR reaction was prepared with 9 μL 5Prime HotMaster Mix (VWR International), 1 μL forward primer (10 μM) ...
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bioRxiv - Systems Biology 2022Quote: ... paired gRNA entry vectors were created by PCR amplification (Red Taq DNA Polymerase Master Mix, VWR Life Science or iProof High-Fidelity DNA Polymerase ...
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bioRxiv - Molecular Biology 2019Quote: ... Double-stranded PCR products were visualized under UV light using a Genoplex Imager (VWR, Darmstadt, DE).
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bioRxiv - Microbiology 2021Quote: ... PCR products were loaded on a 1% agarose gel and stained with EZ-Vision® (VWR). Images were taken using the GelDoc system (Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... Double-stranded PCR products were visualized under UV light using a Genoplex Imager (VWR, Darmstadt, DE). After confirmation of occurrence and correct band sizes corresponding to PCR products (45 µl ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR was performed using the PerfecCTa SYBR Green FastMix (VWR, catalog # 101414-280) on the Step-One Plus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... coli DH5α and positive clones were selected via Colony PCR using Taq polymerase (VWR, Radnor, USA). Correct insertion was checked via Sanger sequencing at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2024Quote: ... a two-fold serial dilution with 1X reaction buffer was conducted in clear PCR tubes (VWR). The methyltransferase reaction was initiated by adding 10 ml of 2x substrate solution (80 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cancer Biology 2023Quote: ... Expression of genes of interest was quantified by real-time PCR using SYBR Green method (VWR QUNT95072). Primers used are detailed in supplementary methods ...
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bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Samples (1 μL) were added to reaction mixtures (19 μL) in a 96-well white PCR plate (VWR) on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were immediately flash frozen in liquid nitrogen in individual 0.2ml thin wall PCR tubes (731-0679, VWR) and stored at -80°C until further use (Supplementary Figure S1) ...
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bioRxiv - Microbiology 2019Quote: Thirty μl of solution A was dispensed into transparent vessels such as PCR tubes (VWR International catalog # 20170-004) or microtiter wells ...
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bioRxiv - Bioengineering 2022Quote: ... combining 1 μL sample with 19 μL reaction mixture per replicate in a 96-well white PCR plate (VWR). For amplicon detection ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s recommendations and was added as a template into a one-step multiplex qRT-PCR assay using Quanta qScript XLT ToughMix with ROX dye (VWR). For that ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
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bioRxiv - Synthetic Biology 2020Quote: ... reactions were incubated in PCR tubes prior to irradiation using a UV-transilluminator (VWR, Dual UV Transilluminator, 80 mW/cm2). Following irradiation ...
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bioRxiv - Developmental Biology 2019Quote: ... by loading 5µl of each PCR product onto a 1% agarose gel with 1:10,000 Gel Red™(Biotium/VWR®) and run for 20 minutes at 100 volts ...
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bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μL of 0.6 mg/mL HCMV gB was mixed with 5 μL of dilute SYPRO Orange working solution in white-walled 96-well PCR plates (VWR), which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
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bioRxiv - Bioengineering 2021Quote: ... 100 μL of dye at each concentration were aliquoted into a strip of 12 optically-clear PCR tubes (VWR 93001-118). The instrument was allowed to reach a steady-state temperature of 69° C before the first strip of tubes was loaded ...
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bioRxiv - Microbiology 2023Quote: ... Then all PCR products were run on 1.5% agarose gel and the PCR product concentrations were assessed based on gel band intensity using GENOSOFT software (VWR International, Belgium). By their concentrations ...
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bioRxiv - Biophysics 2023Quote: ... the top and bottom strand were annealed using a PCR machine (Doppio, VWR®-thermocyclerseries, VWR, part of Avantor, Radnor, US) increasing the temperature to 95 °C (3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were visualised on a 1% agarose gel (run for 30 minutes at 135 V) with GelGreen (VWR, 730-1535) using an ImageQuant™ LAS 500 (GE Lifesciences) ...
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bioRxiv - Microbiology 2024Quote: ... An aliquot (24.5 µL) of the master mix was added to all wells of a 96-well PCR plate (VWR, Radnor, PA). Then ...
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bioRxiv - Genomics 2020Quote: ... Digestion and ligation efficiencies were assessed by gel electrophoresis on a 0.9% agarose gel and quantitative PCR (Brilliant III SYBR qPCR Master Mix, VWR Cat # 97066-528).
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bioRxiv - Bioengineering 2021Quote: ... A 15-µl aliquot of the master mix was combined with 10 µl of sample in a 96-well PCR plate (VWR, 83007-374). The plate was mixed on a Qiagen TissueLyser II for two 25-second intervals at 13 Hz then centrifuged for 2 minutes at 1000 xg.
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were treated either with 800 μM OPA or corresponding concentration of DMSO and heated in parallel in a PCR (VWR, Doppio Gradient) block for 15 min to the three temperatures (37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...
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bioRxiv - Developmental Biology 2021Quote: ... were prepared and stored in 0.2 mL snap strip PCR tubes with dome caps (490003-692, GeneMate – BioExpress, VWR International, Brisbane, CA, USA) at -20 °C prior to qRT-PCR plates preparation ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...