Labshake search
Citations for VWR :
151 - 200 of 281 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... Brains were washed three times in PBS-T and mounted in Mowiol mounting medium on a borosilicate glass side (number 1.5; VWR International). For the Nrx-IV antibody ...
-
bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... 50 µL of the culture were transferred in a sterile tube at selected time point and inoculated on MH agar plate using easySpiral Spiral plater system (VWR). The survivors were counted after an overnight culture ...
-
bioRxiv - Bioengineering 2023Quote: ... Excess streptavidin was washed out three times by pelleting nanovials at 2000xg for 30 seconds on a Galaxy MiniStar centrifuge (VWR), removing supernatant and adding 1 mL of fresh Washing Buffer.
-
bioRxiv - Bioengineering 2023Quote: ... Excess streptavidin was washed out three times by pelleting nanovials at 2000xg for 30 seconds on a Galaxy MiniStar centrifuge (VWR), removing supernatant and adding 1 mL of fresh Washing Buffer.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Washing was carried out three times for 10 min at room temperature with TBS + 0.1% Tween 20 (VWR, United States) and the membrane was incubated with appropriate fluorescent secondary antibodies (listed in Supplementary Table S1 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Karyotyping PCRs were conducted using RedTaq DNA Polymerase 2X Master Mix (VWR Life Science) with the following published primers ...
-
Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... All PCR reactions were conducted using KAPA Hotstart HiFi ReadMix (VWR, Cat#103568-584) with annealing temperature at 62°C (15 sec ...
-
bioRxiv - Molecular Biology 2021Quote: ... The digested tissue was triturated ten times through 18G needle to dissociate the clumps and filtered using a 70μm nylon mesh (VWR, 10199-656) to remove any cell debris ...
-
bioRxiv - Plant Biology 2023Quote: Chlamydomonas cultures harvested at select time points with different temperature treatments were fixed with 0.2% glutaraldehyde (VWR, Cat No. 76177-346). Algal cells were imaged with a Leica DMI6000 B microscope and a 63x (NA1.4 ...
-
bioRxiv - Microbiology 2023Quote: Prior to analysis the supernatants were diluted 40 times with ultrapure water in a glass tube (212−0016, DURAN®, VWR, Denmark ...
-
bioRxiv - Microbiology 2020Quote: ... each well was washed three times with warm PBS and then treated with 500ul PBS with 1% Triton X-100 (AMRESCO, VWR, Randor PA) for 5 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... enterocolitica samples were eluted by washing the wells 3 times with warm PBS followed by treatment with 500 μl PBS with 1% Triton X-100 (AMRESCO, VWR, Randor, PA) for 5 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... Tissues were subsampled using forceps and scalpels either dipped in 96% ethanol and singed three times or cleaned with Eliminase®(VWR, Canada) and nuclease-free water ...
-
bioRxiv - Microbiology 2021Quote: ... Each 20 μL PCR reaction was prepared with 9 μL 5Prime HotMaster Mix (VWR International), 1 μL forward primer (10 μM) ...
-
bioRxiv - Neuroscience 2021Quote: ... 15 min each time, cryopreserved in 30% sucrose (S5-3, Fisher Chemical) in PBS overnight at 4°C and embedded in OCT (VWR International, Wetzlar, Germany). Serial coronal sections (12 μm ...
-
bioRxiv - Microbiology 2020Quote: ... We washed the pelleted biomass 1 – 2 times and resuspended it with 50 µL 1x PBS from which we fixed 2 µL on solidified agarose (VWR, Solon, OH, USA) (1% w/v) ...
-
bioRxiv - Immunology 2021Quote: ... from control and treatment cultures were harvested at both 24- and 48-hour time points and mixed with 400μL of culture media in a 96-deep well storage plate (VWR International, Radnor, PA, USA) and stored frozen at −20°C for cytokine assays by ELISA ...
-
bioRxiv - Systems Biology 2022Quote: ... paired gRNA entry vectors were created by PCR amplification (Red Taq DNA Polymerase Master Mix, VWR Life Science or iProof High-Fidelity DNA Polymerase ...
-
bioRxiv - Molecular Biology 2019Quote: ... Double-stranded PCR products were visualized under UV light using a Genoplex Imager (VWR, Darmstadt, DE).
-
bioRxiv - Microbiology 2021Quote: ... PCR products were loaded on a 1% agarose gel and stained with EZ-Vision® (VWR). Images were taken using the GelDoc system (Bio-Rad).
-
bioRxiv - Molecular Biology 2019Quote: ... Double-stranded PCR products were visualized under UV light using a Genoplex Imager (VWR, Darmstadt, DE). After confirmation of occurrence and correct band sizes corresponding to PCR products (45 µl ...
-
bioRxiv - Plant Biology 2023Quote: ... coli DH5α and positive clones were selected via Colony PCR using Taq polymerase (VWR, Radnor, USA). Correct insertion was checked via Sanger sequencing at Eurofins Genomics (Ebersberg ...
-
bioRxiv - Microbiology 2024Quote: ... a two-fold serial dilution with 1X reaction buffer was conducted in clear PCR tubes (VWR). The methyltransferase reaction was initiated by adding 10 ml of 2x substrate solution (80 mM Tris pH 8.0 ...
-
bioRxiv - Neuroscience 2023Quote: ... Total RNA Kit I (VWR, #CA101319) following manufacturer’s instructions.
-
bioRxiv - Biochemistry 2021Quote: ... AsLPMO10B and VSAL_I0902/AsChi18Bp by PCR amplification using Red Taq DNA polymerase 2× Master mix (VWR, Oslo, Norway) according to the manufacturers protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... Samples (1 μL) were added to reaction mixtures (19 μL) in a 96-well white PCR plate (VWR) on ice ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were immediately flash frozen in liquid nitrogen in individual 0.2ml thin wall PCR tubes (731-0679, VWR) and stored at -80°C until further use (Supplementary Figure S1) ...
-
bioRxiv - Biochemistry 2020Quote: α-syn seeds were generated by sonication of α-syn aggregates at different aggregation time points for 10 minutes in a water bath sonicator (VWR® Symphony™ Ultrasonic Cleaner). 5% (m/m ...
-
bioRxiv - Genetics 2020Quote: ... Tissue DNA isolation kit (Omega Biotek, VWR). For fat pad and synovium ...
-
bioRxiv - Genetics 2020Quote: ... DNA/RNA isolation kit (Omega Biotek, VWR). For blood ...
-
bioRxiv - Microbiology 2019Quote: Thirty μl of solution A was dispensed into transparent vessels such as PCR tubes (VWR International catalog # 20170-004) or microtiter wells ...
-
bioRxiv - Bioengineering 2022Quote: ... combining 1 μL sample with 19 μL reaction mixture per replicate in a 96-well white PCR plate (VWR). For amplicon detection ...
-
bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s recommendations and was added as a template into a one-step multiplex qRT-PCR assay using Quanta qScript XLT ToughMix with ROX dye (VWR). For that ...
-
bioRxiv - Synthetic Biology 2020Quote: ... reactions were incubated in PCR tubes prior to irradiation using a UV-transilluminator (VWR, Dual UV Transilluminator, 80 mW/cm2). Following irradiation ...
-
bioRxiv - Developmental Biology 2019Quote: ... by loading 5µl of each PCR product onto a 1% agarose gel with 1:10,000 Gel Red™(Biotium/VWR®) and run for 20 minutes at 100 volts ...
-
bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
-
bioRxiv - Molecular Biology 2024Quote: ... 15 μL of 0.6 mg/mL HCMV gB was mixed with 5 μL of dilute SYPRO Orange working solution in white-walled 96-well PCR plates (VWR), which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche ...
-
bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
-
bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
-
bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
-
bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
-
bioRxiv - Bioengineering 2021Quote: ... 100 μL of dye at each concentration were aliquoted into a strip of 12 optically-clear PCR tubes (VWR 93001-118). The instrument was allowed to reach a steady-state temperature of 69° C before the first strip of tubes was loaded ...
-
bioRxiv - Bioengineering 2021Quote: ... antibiotic-resistant colonies were re-streaked and verified by colony PCR using specific primers and Red Taq DNA polymerase master mix (VWR International).
-
bioRxiv - Microbiology 2023Quote: ... Then all PCR products were run on 1.5% agarose gel and the PCR product concentrations were assessed based on gel band intensity using GENOSOFT software (VWR International, Belgium). By their concentrations ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR products were visualised on a 1% agarose gel (run for 30 minutes at 135 V) with GelGreen (VWR, 730-1535) using an ImageQuant™ LAS 500 (GE Lifesciences) ...
-
bioRxiv - Biophysics 2023Quote: ... the top and bottom strand were annealed using a PCR machine (Doppio, VWR®-thermocyclerseries, VWR, part of Avantor, Radnor, US) increasing the temperature to 95 °C (3 min ...
-
bioRxiv - Microbiology 2024Quote: ... An aliquot (24.5 µL) of the master mix was added to all wells of a 96-well PCR plate (VWR, Radnor, PA). Then ...
-
bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Minipreps were performed using a peqGold miniprep kit (VWR). WMISH probes were generated via a SP6 or T7 transcription kit (NEB ...