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Citations for VWR :
101 - 150 of 350 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: All steps prior to RT were performed on charged glass slides (VWR). This eliminates the uneasy task of placing a tissue section into the Visium capture area ...
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bioRxiv - Neuroscience 2023Quote: ... blocked for 1hr at RT with 5% normal donkey serum (NDS, VWR) in PBS with 0.25% Triton-X (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... mechanically homogenized and afterward fixed with 2% Paraformaldehyde (10 min, RT; VWR). To minimize clumps ...
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bioRxiv - Molecular Biology 2022Quote: ... Homogenates were sonicated three times for 30s each time at room temperature in a water bath sonicator (VWR, 97043-960). Samples were rested on ice for 30s between each cycle ...
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bioRxiv - Synthetic Biology 2023Quote: ... the PCR plate was sealed with PCR film (VWR 60941-078) and the following reaction was carried out on a thermocycler ...
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bioRxiv - Immunology 2022Quote: ... 1 time with 1ml of 0.1M Na2CO3 (VWR), 1 time with 1ml of freshly prepared 2M Urea (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... and 4 times with isopropanol (VWR,9084-03) and 2 times with distilled water for 3 minutes (min ...
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bioRxiv - Cell Biology 2019Quote: ... Activity was detected using the AceGlow blotting detection system (VWR Peqlab, Erlangen, Germany).
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bioRxiv - Cancer Biology 2020Quote: ... Sections were dried at RT for 30 minutes on a glass coverslip (VWR), and either stored dried at −80°C or directly processed by rehydration in 1XPBS followed by fixation in 4% PFA for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed (4% formaldehyde, VWR International, in PBS; 10 min. at RT), washed (2 × 5 min ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
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bioRxiv - Microbiology 2020Quote: ... solution to a 96 well PCR plate (FLAT 96 WELL PCR PLATE, VWR) and 5 µl of enzyme solution ...
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bioRxiv - Cell Biology 2023Quote: ... strips were covered with Axygen PCR-tube caps (VWR, PCR-02-FCP-C), centrifuged and placed on dry ice until storage at −80°C for further use ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using Amersham ECL Prime Western Blot Detection Reagent (VWR, CA89168-782) and a ChemiDoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Molecular Biology 2023Quote: ... After PCR amplification using NEBNext High-Fidelity 2 × PCR Master Mix (VWR 102500-096) and post-PCR cleanup using SPRI beads (Beckman Coulter A63880) ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed three times 1X PBS (VWR, life science) and resuspended in 1 ml 1X PBS ...
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bioRxiv - Immunology 2022Quote: ... 1 time with 1ml of freshly prepared 2M Urea (VWR) resuspended in 10mM Tris-HCl pH 8.0 (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... and visualization was performed using the AlkPhos Direct Labeling and Detection System (VWR 95038-288) and CDP-Star reagent (VWR 95038-292) ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were imaged by incubating them with the Amersham ECL western blotting detection reagent (VWR) for 1 minute and then imaging every 10 seconds for 5 minutes on the ChemiDoc XRS+ machine (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed 2 times with Histochoice Clearing Agent (VWR, H103) and 4 times with isopropanol (VWR,9084-03 ...
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bioRxiv - Biochemistry 2021Quote: ... PKCδ was visualized using the Amersham Enhanced Chemiluminescence Western blotting detection system (VWR, Mississauga, ON, Canada). Band intensity was quantified using Image J software ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times with PBS with 0.1% Tween 20 (VWR International AB) (PBST) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were dounced 20 times in a 7ml stainless tissue grinder (VWR) and then spun at 200g for 5min to pellet large debris ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT before being mounted on a microscope slide using VECTASHIELD (VWR, #H-1000) and sealed in nail polish ...
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bioRxiv - Immunology 2023Quote: ... into empty 96-well PCR plates (VWR). FACSDiva software (Becton Dickinson ...
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bioRxiv - Cell Biology 2021Quote: ... Alkaline phosphatase coupled secondary antibodies were used for all blots followed by direct detection with NBT/BCIP (VWR) substrates on PVDF membranes.
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bioRxiv - Cancer Biology 2022Quote: ... HAF109) were incubated for 1 h prior to detection with SuperSignal West Pico Plus Chemisubstrate (VWR International, PIER34577).
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bioRxiv - Molecular Biology 2022Quote: ... for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent, VWR).
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bioRxiv - Developmental Biology 2022Quote: ... Stained slides were washed 3 times in PBS and No.1 coverslips (VWR) were added using ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... then disrupted 5 times with a dounce homogenizer (VWR cat. no. 62400-595). Following mechanical disruption ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were washed 4 times with 0.1% NP-40 substitute (VWR, 97064-730) in tris-buffered saline (TBS) ...
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bioRxiv - Biochemistry 2024Quote: ... The plate was washed three times and TMB (VWR International, 50-76-03) substrate was added ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Zoology 2020Quote: ... PCR reactions were performed in a 55 μl volumes containing: 25 μl Hot Start PCR-to-Gel Master Mix (VWR brand); 2 μl of primer mix (25 pmol each of forward and reverse primer) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times and fixated using 1% formaldehyde (Avantor, VWR, Denmark). Fluorescent intensity was measured with a NovoCyte 3000 Analyzer equipped with three lasers (405 ...
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bioRxiv - Immunology 2023Quote: ... All samples were diluted ten times in low EDTA TE-buffer (VWR, cat.: APLIA8569.0500) in a total volume of 20 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... an adapter PCR reaction was performed as follows: All 8 tubes of a 50 µL PCR strip tube (VWR, catalog # 490003-606) were filled with 2500 ng DNA template ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed 5x with TBST and signal was detected using ECL Western Blotting detection reagent (GE) or ECL prime (VWR).
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Microbiology 2020Quote: Samples were incubated with 10 mM CCCP (prepared in 50% DMSO) for 30 min at RT followed by addition of 10 mM Nile Red (VWR) at 37°C and shaken at 200 rpm for 30 min ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The membranes were washed 3x in PBST at RT and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (VWR, Amersham ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were washed in 1x TBS-T and incubated for 1 hour at RT in the dark with species-specific secondary antibodies (Rb-680; 926-68071; Ms-800 926-32212) (VWR) at 1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated on ice in 96-well PCR plates (VWR) for 30 min and then heat-shocked for 1 min at 42 °C in a BioRad T100 Thermal cycler ...
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bioRxiv - Biophysics 2021Quote: ... in a 384-well PCR microplate (VWR, 10011-194), with a final volume of 15 μL per well ...
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bioRxiv - Synthetic Biology 2020Quote: ... in RNase-free 0.2 ml PCR tubes (VWR International) or 1.5 ml DNA LoBind tubes (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR bands were visualized on 2% agarose (VWR, 97062) in TBE (VWR ...
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bioRxiv - Cell Biology 2020Quote: ... the coverslips were washed three times with PBS and mounted on microscope slides (VWR International) in DAPI-containing Prolong Gold (Invitrogen) ...