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Citations for VWR :
51 - 100 of 276 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... into empty 96-well PCR plates (VWR). FACSDiva software (Becton Dickinson ...
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bioRxiv - Cell Biology 2021Quote: ... Alkaline phosphatase coupled secondary antibodies were used for all blots followed by direct detection with NBT/BCIP (VWR) substrates on PVDF membranes.
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bioRxiv - Cancer Biology 2022Quote: ... HAF109) were incubated for 1 h prior to detection with SuperSignal West Pico Plus Chemisubstrate (VWR International, PIER34577).
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bioRxiv - Molecular Biology 2022Quote: ... for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent, VWR).
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bioRxiv - Developmental Biology 2022Quote: ... Stained slides were washed 3 times in PBS and No.1 coverslips (VWR) were added using ProLong Gold Antifade Mountant (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... then disrupted 5 times with a dounce homogenizer (VWR cat. no. 62400-595). Following mechanical disruption ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were washed 4 times with 0.1% NP-40 substitute (VWR, 97064-730) in tris-buffered saline (TBS) ...
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bioRxiv - Biochemistry 2024Quote: ... The plate was washed three times and TMB (VWR International, 50-76-03) substrate was added ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Zoology 2020Quote: ... PCR reactions were performed in a 55 μl volumes containing: 25 μl Hot Start PCR-to-Gel Master Mix (VWR brand); 2 μl of primer mix (25 pmol each of forward and reverse primer) ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed three times and fixated using 1% formaldehyde (Avantor, VWR, Denmark). Fluorescent intensity was measured with a NovoCyte 3000 Analyzer equipped with three lasers (405 ...
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bioRxiv - Immunology 2023Quote: ... All samples were diluted ten times in low EDTA TE-buffer (VWR, cat.: APLIA8569.0500) in a total volume of 20 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... an adapter PCR reaction was performed as follows: All 8 tubes of a 50 µL PCR strip tube (VWR, catalog # 490003-606) were filled with 2500 ng DNA template ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed 5x with TBST and signal was detected using ECL Western Blotting detection reagent (GE) or ECL prime (VWR).
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Microbiology 2020Quote: Samples were incubated with 10 mM CCCP (prepared in 50% DMSO) for 30 min at RT followed by addition of 10 mM Nile Red (VWR) at 37°C and shaken at 200 rpm for 30 min ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The membranes were washed 3x in PBST at RT and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (VWR, Amersham ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were washed in 1x TBS-T and incubated for 1 hour at RT in the dark with species-specific secondary antibodies (Rb-680; 926-68071; Ms-800 926-32212) (VWR) at 1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated on ice in 96-well PCR plates (VWR) for 30 min and then heat-shocked for 1 min at 42 °C in a BioRad T100 Thermal cycler ...
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bioRxiv - Biophysics 2021Quote: ... in a 384-well PCR microplate (VWR, 10011-194), with a final volume of 15 μL per well ...
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bioRxiv - Synthetic Biology 2020Quote: ... in RNase-free 0.2 ml PCR tubes (VWR International) or 1.5 ml DNA LoBind tubes (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR bands were visualized on 2% agarose (VWR, 97062) in TBE (VWR ...
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bioRxiv - Cell Biology 2020Quote: ... the coverslips were washed three times with PBS and mounted on microscope slides (VWR International) in DAPI-containing Prolong Gold (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... The perineal region was cleaned three times with ethanol and a betadine scrub (VWR, AJ159778), and sterile dissecting shears were used to make a 4–5 mm incision in this region ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed three times in PBS and mounted on microscope slides (VWR International) in Prolong Gold (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... 4 mL per time point) in 6-well polystyrene well plates (Greiner Bio-One; VWR). After 4 and 8 hours ...
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bioRxiv - Bioengineering 2023Quote: ... Cell monolayers were then rinsed three times with PBS and fixed with 4% paraformaldehyde (VWR) for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were afterwards washed several times in quencher solution [10 mM sodium ascorbate (VWR), 10 mM sodium azide (VWR) ...
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bioRxiv - Physiology 2019Quote: ... Paraffin-embedded tissue sections were then sectioned at 100 μm intervals using a Leica RM2125 RTS microtome and collected onto 76 x 26 mm StarFrost slides (VWR, UK). The slides were baked at 37°C overnight before Hemotoxylin and Eosin (H&E ...
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bioRxiv - Bioengineering 2021Quote: ... Stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and mounted on a poly-L-lysine coated coverglass (VWR, 48393-241) for imaging (Fig ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Paraffin-embedded tissue sections were then sectioned at 100 µm intervals using a Leica RM2125 RTS microtome and collected onto 76 x 26 mm StarFrost 624 slides (VWR, UK). The slides were baked at 37°C overnight before Haematoxylin and Eosin (H&E ...
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bioRxiv - Biophysics 2023Quote: ... and 1%(v/v) ammonium persulfate (APS) for 60 minutes at RT on plasma cleaned glass cover slips (No.1, 25mm Ø, VWR) that were pre-treated with 3-aminoproyltriethoxysiliane (APTES ...
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bioRxiv - Systems Biology 2021Quote: ... Primers were arrayed in skirted 96 well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Systems Biology 2020Quote: ... Primers were arrayed in skirted 96 well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Reactions were incubated in a nuclease-free PCR tube (VWR) in a ThermalCycler (C1000 Touch ...
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bioRxiv - Microbiology 2023Quote: ... each amplicon was PCR amplified using the RedTaq polymerase (VWR) and cloned in the plasmid pCR2.1 using the TOPO-TA CloningTM Kit (Invitrogen).
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bioRxiv - Systems Biology 2023Quote: ... Primers were arrayed in skirted 96-well PCR plates (VWR) using an acoustic liquid handling robot (Labcyte Echo 550 ...
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bioRxiv - Molecular Biology 2021Quote: 2 x 107 BCBL-1 cells per time point were crosslinked with 2% formaldehyde (VWR M134) in prewarmed 1X phosphate buffered saline (PBS ...
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bioRxiv - Plant Biology 2021Quote: Cultures harvested at select time points were fixed with 0.2% glutaraldehyde (VWR, Cat No. 76177-346). Cells were imaged with a Leica DMI6000 B microscope and a 63x (NA1.4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Stained coverslips were then washed three times with 1x PBS and mounted on microscope slides (VWR) with homemade MOWIOL mounting medium ...
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bioRxiv - Immunology 2023Quote: ... The cells were fixed by dipping 10 times in pre-cooled ice-cold acetone (VWR, #20066.296) in a glass tray ...
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bioRxiv - Microbiology 2021Quote: ... Agar overlay lawns for passages were prepared by aliquoting 100 ul of host overnight culture (4ml 2216MB, colony inoculum from streak on 2216MB with 1.5% Bacto Agar, shaken overnight at RT at 250rpm on VWR DS500E orbital shaker) onto a standard size bottom agar plate and adding 2.5 ml of molten 52°C top agar as in the bait assay ...
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bioRxiv - Neuroscience 2020Quote: ... immersed in 1x PBS at RT for 30 seconds and transferred to 25% Optimal Cutting Temperature compound (OCT, TissueTek; VWR 25608-930), followed by 50% OCT and then embedded in 100% OCT and placed in a cryomold ...
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bioRxiv - Cell Biology 2023Quote: ... The stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and finally mounted on poly-L-lysine coated cover glasses (VWR, 48393-241) for triple-view line confocal super-resolution imaging20.
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bioRxiv - Cancer Biology 2022Quote: ... beads are washed three times with 1X sodium chloride-sodium phosphate-EDTA buffer (SSPE, VWR, #VWRV0810-4L), added to the purified PCR product ...
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bioRxiv - Neuroscience 2020Quote: ... then washed three times with 0.1 M PB before being mounted onto Superfrost slides (VWR, Radnor, PA) with 0.1% gelatin and coverslipped with Prolong Gold antifade reagent (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were then rinsed with PBS 1x and washed 5 times with PBS-triton 0.8% (28817295, VWR) for 10 min with slight agitation ...