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Citations for VWR :
51 - 70 of 70 citations for pVectOZ GFP Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: HEK 293T cells were cultured in six-well plates and double transfected using jetPRIME transfection reagent (VWR International, Radnor, PA, USA) with pCMV-luciferase ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentivirus-containing medium was collected from HEK293T cells 48h and 72h post-transfection and added to recipient cancer cells after filtration using a 0.45 µM filter (VWR-Corning, 431220). 48h post infection ...
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bioRxiv - Cancer Biology 2021Quote: MCF7 KRT19 KO cells that were generated using the CRISPR/Cas9 system (Sharma et al., 2019a) and isogenic parental control cells (ATCC, Manassas VA) were grown in Dulbecco’s Modified Essential Medium (VWR Life Science) containing 10% fetalgro bovine growth serum (RMBIO ...
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bioRxiv - Neuroscience 2022Quote: ... 25 ml/min) diverted into the headspace of a control vial filled with solvent (paraffin oil, J.T. Baker, VWR #JTS894-7) before it was reunited with the carrier stream (250 ml/min) ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 Flp-in T-REx/GFP-LRRK1 cells were seeded at 2.5 x 105 cells/well in 6 well plates on glass coverslips (VWR, 631-0125 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with retroviral Gag-pol (8 ug) and VSV-G (4 ug) packaging plasmids and pBabe vector (8 ug) using PEI Max transfection reagent (VWR, 75800-188). After 24 h ...
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bioRxiv - Microbiology 2023Quote: ... were transfected into single wells (3.5 cm) of the Corning Costar 6-well cell culture plate containing HEK293T/17 cells using the JetPrime Transfection reagent (VWR, Randor, PA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: One mL of BMDM from lipin-1mKO and littermate control mice at a concentration of 1×106/mL was placed into falcon tubes (VWR-60818-500). Cells were either treated with or without IL-4 (40ng/mL ...
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bioRxiv - Genetics 2021Quote: The 1.8 SRE-Elk cell line was generated by lentiviral transduction of 1.8 XX mESCs with the pLenti-SRE/Elk-GFP-PEST-Hygro plasmid (Supp. Table S4) followed by Hygromycin (250 ng/µl, VWR) selection ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 3 ug of DNA in the same superfolder GFP-containing pEG BacMam vector used for large-scale purification and 9 μL FuGene HD (VWR) diluted in 150 μL OptiMEM and added dropwise to the cells after a 5 min incubation ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibodies (1:1000 goat anti-GFP from Novus Biologicals or 1:10 000 rabbit anti-RFP from VWR (Rockland) were incubated with PGT overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: Hypothalamic progenitors differentiated from the HUES9-POMC-GFP cell line were re-plated onto 24 mm round glass coverslip (VWR) that has been pre-treated with 5 M hydrochloric acid and double coated with both 0.01% poly-L-ornithine hydrobromide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... 12 µg of plasmid DNA (ratio 1:10 for guide-RNA and dCas9-construct containing plasmids) was diluted into 5 µl of sterile 10 % glucose and added to diluted In vivo-jetPEI® reagent (#101000040 VWR, Polyplus-transfection). The solution was mixed by pipetting and incubated at RT for 15 min ...
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bioRxiv - Physiology 2021Quote: ... the blood was split into aliquots of 600 µl that were loaded into individual tonometers and treated with either: i) a carrier control (0.25% dimethyl sulfoxide, DMSO; VWR BDH 1115; Radnor, USA), ii ...
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bioRxiv - Developmental Biology 2024Quote: ... we generated a sterile circuit by pumping 50 mL of medium from a 50 mL conical tube reservoir using a Masterflex Ismatec Reglo Independent Channel Control Peristaltic Pump (MFLX78001-80, 4-channel, 8-roller; Avantor/VWR, Radnor, PA, USA) fitted with 1.52 mm inner diameter 3-stop tubing (95625-36 ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic Hydra from strains Tg(actin1:GFP)rs3-in and Tg(tba1c:mNeonGreen)cj1-gt were dissociated using Pronase E (VWR, E629-1G) as previously described (Siebert et al. ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were then split into a negative control (no antibiotic) or antibiotic treatment (2 mL of culture per each treatment, using plastic Falcon tubes, VWR® Cat. No. 352059). After addition of the antibiotic from concentrated stocks ...
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bioRxiv - Microbiology 2019Quote: ... Colonies of each bacterial test isolate and a positive and negative control isolate were harvested with a 1 μl loop (VWR International, catalog # 12000-806) from blood agar (BD Diagnostics Systems ...
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bioRxiv - Bioengineering 2022Quote: ... WM989 A6-G3 H2B-GFP and WM989 A6-G3 RC4 cells were mixed together and plated on coverslips (VWR, 16004-098, 24×050mm, No. 1 coverglass) with 24-well silicone isolators (Grace Bio-Labs ...