Labshake search
Citations for VWR :
101 - 150 of 278 citations for VEGF C Human 196a.a HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... then incubated overnight at 37°C with gentle agitation (VWR Thermal Shake Touch, 900 rpm). Digested peptides extracted into supernatant and transferred to VWR non-stick microcentrifuge tubes ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and subsequently filtered into 1.5 mL short thread vials with crimp caps (VWR International GmbH ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were grown overnight at 37°C at 100rpm in LB (5mg/ml NaCl, VWR) with 50μg/ml kanamycin or 50μg/ml chloramphenicol (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... followed by 2 x 2-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...
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bioRxiv - Neuroscience 2023Quote: ... at -20 °C and mounted on glass slides (48311-703, VWR Superfrost Plus Micro Slide). The glass slides were kept on dry ice and stored at -80 °C until further processing.
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bioRxiv - Cancer Biology 2023Quote: ... Staining steps were performed at room temperature (20-25°C) on superfrost glass slides (VWR) in double adhesive GeneFrame Chambers (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cultures were maintained in an 37°C incubator with 5% CO2 and relative humidity (VWR).
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bioRxiv - Biochemistry 2023Quote: ... The reaction progressed overnight at 4°C supplemented with ATP Regeneration Mix (creatine phosphate (VWR) and creatine kinase (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated at 37°C for 30 min in a benchtop incubator (INCU-Line, VWR). The Formamide Wash Buffer was then aspirated ...
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bioRxiv - Molecular Biology 2024Quote: ... E3 medium was maintained at 28 °C in a 14 cm diameter Petri dish (VWR), into which 72 hpf embryos were placed in the centre of the dish individually ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then imaged on a C-DiGit Chemiluminescent Western Blot Scanner (103375-240, VWR) using SuperSignal West Pico PLUS Chemiluminescent Substrate (PI34577 ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... Wells of the 12-well microtiter plate (VWR 734-2324 T C-treated 12-well plates) were prepared just before inoculation and imaging by coating the bottom of each well with 15 µl of Con A and allowing the wells to dry ...
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bioRxiv - Neuroscience 2020Quote: ... cryopreserved in 30% sucrose overnight at 4°C and embedded in OCT (VWR International, Wetzlar, Germany). Serial coronal sections (14 μm ...
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bioRxiv - Immunology 2019Quote: ... All of the cells were cultured in humidified incubators at 37 °C with 5% CO2 (VWR).
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bioRxiv - Cell Biology 2020Quote: ... These samples were then incubated for 90 min at 40°C on an orbital shaker (VWR) at 1500 rpm ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were incubated at 30°C for 18 h in a stable incubator (INCU-Line, VWR), unless otherwise stated ...
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bioRxiv - Microbiology 2022Quote: ... in 1x PBS and incubated with shaking overnight at 4 °C (Digital Platform Rocker Shaker, VWR). Following overnight fixation ...
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bioRxiv - Microbiology 2022Quote: ... All cells were maintained at 37 °C in a 5% CO2 humidified incubator (VWR, Mississauga, Ontario). For subculturing ...
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bioRxiv - Bioengineering 2023Quote: ... Particles were measured at 25°C in a black polystyrene microwell plate (Greiner Bio-One; VWR). Separately ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated at 37 °C at 1,000 rpm in an Incubating Microplate Shaker (VWR, # 97043-606) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were spun and then incubated at 91°C for 3 min on heat blocks (VWR), then 60°C for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... coli cells were grown at 37 °C with aeration in either LB (Luria-Bertani broth; VWR) or LA (Luria Agar ...
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bioRxiv - Biochemistry 2019Quote: ... spectra of native, human BACE1 (6.12 μM, 30 μl; Acro Biosystems, USA) in 50 mM sodium acetate (pH 4.0; VWR, UK) was obtained using a J-1500 Jasco CD spectrometer and Spectral Manager II software ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Human foreskin fibroblasts (HFF, kindly provided by Denise Galloway) were maintained in Minimum Essential Media-α (MEM-α; VWR) supplemented with 20% FBS (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... in 1X PBS at 4°C before embedding them in Optimal Cutting Temperature (OCT) Compound (VWR Chemicals) and snap freezing them with isopentane on dry ice ...
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bioRxiv - Neuroscience 2020Quote: ... and DNase I (20μg/ml) at 37°C for 75 minutes in an incubating mini shaker (VWR). The digested brain material was pelleted by centrifugation and resuspended in 20% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: All strains were grown on Czapek Dox Agar (CDA) at 37°C in an incubator (VWR International) in the dark over four days ...
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bioRxiv - Neuroscience 2022Quote: ... pH 8.4) in a 35 mm Mat-Tek glass coverslip bottom dish (VWR P35G-0-14-C) at an angle perpendicular to the liquid’s surface ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 48-hour incubation at 4°C in 50% optimal cutting temperature (OCT; VWR, #C361603E)/30% sucrose/phosphate buffer solution prior to freezing in 100% OCT using liquid nitrogen-cooled 2-methylbutane (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... size fractions were shaken overnight at 56 °C using the incubation chamber INCU Line ILS 6 (VWR). After the lysate cooled down ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with an antibody against RFP (1:1000, Rockland (VWR), catalog #RL600-401-379) ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 hours at 56°C and 50 μg of RNase A (VWR International, Radnor, PA, USA) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... counted and kept frozen in aliquots at −150°C in FBS with 10 % dimethyl sulfoxide (DMSO, VWR) for future experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by 3 x 1-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...
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bioRxiv - Physiology 2022Quote: ... Samples were kept at -20°C throughout the extractions in an Iso-Therm System (VWR #20901-646). Fifteen milligrams of liver were homogenized in 200 µL of a 3:3:2 solution of acetonitrile:isopropanol:water (MeCN:IPA:H2O ...
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bioRxiv - Biophysics 2023Quote: ... were soaked for 1 hour at ~ 60°C in 2% v/v cleaning detergent (MICRO-90, VWR), thoroughly rinsed with 18.2 MΩ ultra-pure water ...
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bioRxiv - Bioengineering 2024Quote: ... The blood placed into a 0.5 ml Greiner Bio-1 minicollection tubes (VWR, International L. L. C.) with ethylene diamine tetra-acetic acid (EDTA ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
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bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...
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bioRxiv - Microbiology 2019Quote: ... strains [69], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
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bioRxiv - Microbiology 2023Quote: Toxoplasma strain TATi-RHΔku80 [41] was cultured in human foreskin fibroblasts in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA, USA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Cell Biology 2019Quote: ... lot# 177772) and shaken at 37 °C overnight in a shaker at 1200 rpm (VWR Cat#12620-942).
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bioRxiv - Biophysics 2021Quote: ... was deposited on C-Flat holey carbon grids (hole size: 1.2 µm; spacing: 1.3 µm; mesh: 400, (VWR)) and a FEI Vitrobot Mark IV was used to plunge freeze the grids ...
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bioRxiv - Microbiology 2021Quote: ... were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (VWR) and 100 µg/ml of penicillin-streptomycin (Wisent) ...