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Citations for VWR :
151 - 200 of 282 citations for Tumor necrosis factor receptor superfamily member 3 TNFRSF3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... (3) we then pipette the solutions vigorously in a 20 mL scintillation glass vial (VWR) with a hydrophobic coating which is introduced by incubation with Rain-X (ITW Global Brands ...
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bioRxiv - Immunology 2021Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60 μl ...
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bioRxiv - Immunology 2021Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60μl ...
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bioRxiv - Immunology 2021Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were selected for five days in presence of 3 μg/ml puromycin (VWR, CAYM13884). A near 100% GFP-positive population was confirmed via microscopy (ZOE Fluorescent Cell Imager ...
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bioRxiv - Cancer Biology 2023Quote: ... fluorescence intensity (∼485 nm/∼530 nm) was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, catalog # 97062-376, VWR) (5 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: NGM plates with a final concentration of 1μM auxin (indole-3-acetic acid, VWR AAA10556-36) were made according to [60] ...
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bioRxiv - Genomics 2020Quote: ... 3 15min washes in 5x SSCT (1:10,000 DAPI, VWR, TCA2412-5MG, in the second wash), and storage/imaging in 5x SSCT ...
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bioRxiv - Genetics 2019Quote: ... Digested products were electrophoresed on 3% agarose gels using Agarose SFR™ (VWR Life Science, USA) for 3 hours at 80V ...
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bioRxiv - Biochemistry 2021Quote: ... The gel was stained with Ethidium Bromide and visualised using a Smart 3 GelDoc System (VWR).
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bioRxiv - Genomics 2021Quote: ... 3 15min washes in 5x SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5x SSCT ...
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bioRxiv - Microbiology 2023Quote: ... with 100 μg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-Gal, VWR), or with 0.1 μM CdCl2 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were spun and then incubated at 91°C for 3 min on heat blocks (VWR), then 60°C for 20 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The 3’ end enriched libraries were constructed using KAPA HyperPlus Library Preparation Kit (VWR International, KK8513) with 6 cycles of PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (3 x 5 min) and mounted on slides (Superfrost Plus, VWR) with mounting medium (Fluoromount ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample-beads were then washed with 3 mL 75-80% ethanol (VWR Chemicals, PA, USA) and again with 2 mL 75-80% ethanol ...
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bioRxiv - Biochemistry 2019Quote: ... spectra of native, human BACE1 (6.12 μM, 30 μl; Acro Biosystems, USA) in 50 mM sodium acetate (pH 4.0; VWR, UK) was obtained using a J-1500 Jasco CD spectrometer and Spectral Manager II software ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Human foreskin fibroblasts (HFF, kindly provided by Denise Galloway) were maintained in Minimum Essential Media-α (MEM-α; VWR) supplemented with 20% FBS (GE Healthcare ...
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bioRxiv - Genetics 2019Quote: ... All digested products were electrophoresed on 3% agarose gels using Agarose SFR™ (VWR Life Science, USA) for 3 hours at 80V ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Plant Biology 2021Quote: ... 3.5 mL of 18.2 MΩ deionised water (Elga PURELAB flex 3 system, VWR International, Edmonton, AB, Canada) was then added to effect a separation into a polar methanol/water layer and a chloroform layer ...
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bioRxiv - Microbiology 2021Quote: HEK 293T cells (3×105 cells) were plated on the sterilized round 15mm #1 glass coverslips (VWR) in 12-well plates and seeded for 24hours prior to transfection ...
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bioRxiv - Bioengineering 2020Quote: ... Then we denatured the phage protein coat by adding 7.5 ml of 3 M KOAc (VWR international), pH 5.5 ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed in PBS (3×10 min) and mounted on Superfrost Plus slides (VWR, Radnor, PA) using Fluoromount G (Southern Biotechnology Associates ...
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bioRxiv - Bioengineering 2021Quote: ... On day 3 the cells were collected using Trypsin ethylenediamine tetraacetic acid (EDTA) (VWR, Chicago, IL, USA). Cells were mixed with either GelMA (10% w/v final ...
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bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by 3 x 1-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...
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bioRxiv - Cell Biology 2022Quote: ... The micropipette was then sandwiched between a 1-mm glass cover slide (3”x1”x 1mm; VWR) and a #1.5 glass cover slip (24×60 mm ...
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bioRxiv - Neuroscience 2022Quote: ... the digestion mixture was diluted 1:3 with 10% Acetonitrile in MS grade water (VWR Cat#83645.320), followed by sonication in the Bioruptor Plus sonication system and incubation with 1 μg of LysC and 2 μg of Trypsin (Promega Cat#V511A ...
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bioRxiv - Microbiology 2024Quote: ... Moore swabs were constructed by folding 3 by 24-inch strips of cheese cloth (VWR, Radnor, PA) 8 times to form an 8-ply square pad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
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bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...
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bioRxiv - Microbiology 2019Quote: ... strains [69], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
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bioRxiv - Microbiology 2023Quote: Toxoplasma strain TATi-RHΔku80 [41] was cultured in human foreskin fibroblasts in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA, USA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 15 minutes with 5X SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5X SSCT ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Biochemistry 2019Quote: ... 0.4% Zinc) and resuspended in 5 mM HCl at 3 mg/ml (Cat# 20252.244, VWR Chemicals, Dresden, Germany). The purity of insulin was confirmed by mass spectrometry under denaturing conditions where only monomeric and dimeric insulin with a mass of 5803.651 +/-0.003 Da was detectable ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated overnight at room temperature on a shaker in presence of 3 μg LysC Endopeptidase (VWR). Next ...
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bioRxiv - Genomics 2020Quote: ... were co-transfected with the respective lentiviral expression vector (∼1:1:3 molar ratio) using Trans-IT (VWR) and OptiMEM (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μm-thick sections were obtained and counterstained with Liquid-Stable Nuclear Fast Red (VWR Life Science AMRESCO).
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was obtained by individually centrifuging 250 µl of each dilution at 5000 × g for 3 minutes through a 0.2 µm spin filter (Centrifugal Filter, Modified Nylon 0.2 μm, VWR). Both sample types ...
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bioRxiv - Bioengineering 2022Quote: ... Tissues were homogenized by 3 × 10 s pulses at maximum speed with a VDI12 rotorstator (VWR, Dresden, Germany). Protein extracts were quantified by a Bradford protein assay (Bio-Rad Laboratories ...