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Citations for VWR :
1 - 50 of 764 citations for Trypsin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2019Quote: ... After blocking with 1% bovine serum albumin (BSA) (VWR, Radnor, PA, US), the membranes were probed with 1 μg/mL mAb 9G3 against the glycosylated epitope ‘KEPAPTTT’ in the lubricin mucin domain (Merck KGaA ...
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bioRxiv - Bioengineering 2022Quote: ... they were split into new flasks at one tenth of the density using trypsin (Trypsin EDTA 1X 0.25% Trypsin/ 2.21mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium, VWR 45000-664).
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bioRxiv - Cell Biology 2022Quote: ... Blots were blocked at room temperature for 1 h using Rockland blocking buffer (VWR). Primary PDI antibody (Abcam ...
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bioRxiv - Immunology 2020Quote: ... Plates were then blocked with 100μl of blocking buffer (1% Casein in PBS; VWR) for 1.5hr at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were blocked for 1 h with Rockland blocking buffer (VWR, Catalog #RLMB-070) then incubated with PDI antibody (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 60-minute blocking with donkey serum (1:10 diluted in 1% BSA; VWR, Pennsylvania, USA). Incubation with the primary antibody was performed overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... soybean trypsin inhibitor (VWR Amresco) was added at 50 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... The antimicrobial peptide C18G (VWR) was added at 5 µg/mL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and digested further with trypsin (VWR) diluted 1:5 in PBS for 5 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the peptides were re-dissolved in 10 μl 1% formic acid (VWR Ltd., Radnor, PA, USA) and were separated in a 180 min water/acetonitrile gradient using an Easy nLC 1200 nano UPLC (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with mouse anti-α-tubulin antibody (DM1A, VWR; #PI62204; 1:1000 in blocking buffer) for 1h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Membrane blocking was performed for 1 h at room temperature in 5% bovine serum albumin (BSA; VWR) in Tris-buffered saline containing Tween-20 (TBST) ...
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bioRxiv - Genomics 2022Quote: ... and Trypsin (VWR, Cat. No. 45000-666) for 5 minutes at 37° C ...
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bioRxiv - Molecular Biology 2019Quote: ... After blocking with 5% BSA (VWR LIFE SCIENCE) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and trypsinized (Trypsin EDTA 1X 0.25% Trypsin/ 2.21mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium, VWR 45000-664). Cell density was determined by optical density with an automated cell counter (Bio-Rad TC20) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Immunology 2023Quote: Back skin was incubated with 0.5% Trypsin (VWR) for 45min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed midguts were blocked for 1 h in blocking solution (1% bovine serum albumin, Cat# A8806, Sigma-Aldrich, St. Louis, MO; 1% normal donkey serum, Cat# 102644-006, VWR, Radnor, PA. in PBS). Then the primary antibody (1:1000 ...
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bioRxiv - Pathology 2021Quote: ... After blocking with 3% bovine serum albumin (BSA, VWR Chemicals, UK) in TBS-tween ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by blocking by 3% bovine serum albumin (BSA, VWR, 421501J) in phosphate-buffered saline-Tween 0,1% (PBS-T 0,1%) ...
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bioRxiv - Immunology 2021Quote: ... Slices were stained in a blocking buffer composed of TritonX100 (VWR) 0.1-0.3 % ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissue was first blocked for 30 min using a blocking buffer (TBS with 1% BSA (VWR, 22013, Bovine Serum Albumin (BSA), fraction V ...
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bioRxiv - Immunology 2020Quote: ... Unbound protein was washed away with PBST (1x PBS with 0.5% Tween) followed by blocking with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 h at room temperature with mild shaking (55 rpm) ...
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bioRxiv - Bioengineering 2023Quote: ... the strips were immersed in a blocking buffer (1% BSA and 0.05% Tween 20) for one hour on an orbital shaker (no. 57018-754VWR; VWR International, Brisbane, CA) set at 150 rpm and thereafter dried overnight at 4° Celsius ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with the LI-COR Biosciences Intercept Blocking buffer (VWR International) for one hour at room temperature and incubated with primary antibodies overnight at 4 °C on a rocker ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with blocking solution PTWH (0.2% Tween-20 (Cat. #: 28829.183, VWR) and 10 mg/L heparin (cat ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were dissociated in 250 μl of 0.25% Trypsin-0.02% EDTA (VWR) at 37°C while shaking at 500 rpm ...
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bioRxiv - Bioengineering 2023Quote: Single cell suspensions were prepared from clusters incubation in trypsin/EDTA (VWR). 20% of the total volume of fetal bovine serum was added and the tube placed on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... for blocking and with 0,2% Tween® 20 added for antibody dilutions (28829.296, VWR), washes were done with phosphate-buffered saline tween (PBST ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with a blocking solution (2 % bovine serum albumin (BSA; VWR) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... After blocking with 5% bovine serum albumin (BSA; VWR Life Science, Radnor, PA, USA) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated in blocking buffer consisting of 5% bovine serum albumin (BSA; VWR) dissolved in 1X Tris-buffered saline (TBS ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were dissolved in titanium dioxide (TiO2) loading buffer (80% acetonitrile (ACN, VWR), 5% Trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2021Quote: ... Blocking buffer and primary antibody incubation solution was 5% non-fat dairy milk powder (VWR) or 5% (w/v ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were detached for passaging by 5 min incubation with trypsin-EDTA (0.25%, VWR). MDA-MB-231 cells were used in studies between passages 45 and 50 ...
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bioRxiv - Molecular Biology 2023Quote: ... Phosphorylated peptides were eluted twice using 150 µl 300 mM ammonium hydroxide (VWR, 1.05432.1000), eluates were united and immediately acidified with conc ...
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bioRxiv - Molecular Biology 2023Quote: ... Phosphorylated peptides were eluted twice using 150 µl 300 mM ammonium hydroxide (VWR, 1.05432.1000), eluates were united and immediately acidified with conc ...
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bioRxiv - Cell Biology 2021Quote: ... and LiCOR Intercept Tris buffered saline (TBS) blocking buffer (Catalog # 103749-018) were purchased from VWR/Avantor ...
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bioRxiv - Molecular Biology 2019Quote: ... Completed DMEM was added to inactivate trypsin and cells were collected into 15 mL tubes (VWR). The tubes were centrifuged at 300 rcf for 5 minutes to pellet cells and total protein was extracted by resuspending each pellet in 1 mL of RIPA buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... Trypsin was then quenched by adding 4 mL MEF media (Dulbecco’s Modified Eagle Medium (DMEM, VWR) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Systems Biology 2021Quote: BJ-5ta cells were lifted from T-150 culture flasks using 0.25% trypsin (VWR 45000-664) for 5–10 min ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated with the primary antibody diluted in Intercept Blocking buffer supplemented with 0.1 % Tween20 (Carl Roth, 9127.1) and 0.2 % sodium dodecyl sulfate (SDS; VWR, A3942) for 16 h at 4° C ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated in blocking solution [5% milk in PBST (PBS (pH 7.4) with 0.1% Tween-20 (VWR))] for 2 h and probed overnight with the following primary antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... primary antibodies were added to the blocking solution for 20 minutes while rocking (VWR analog rocker 10127-876), in the dark and on ice ...
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bioRxiv - Bioengineering 2021Quote: ... On day 3 the cells were collected using Trypsin ethylenediamine tetraacetic acid (EDTA) (VWR, Chicago, IL, USA). Cells were mixed with either GelMA (10% w/v final ...
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bioRxiv - Cell Biology 2021Quote: ... All washing steps between blocking or chemical incubation were performed with PBS supplemented with 0.5 % Tween-20 (VWR, 8.22184).
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were blocked for 90 minutes at room temperature in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). All the antibodies used in this study and their concentrations are listed in File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...