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Citations for VWR :
51 - 100 of 199 citations for Total Phosphatase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Synthesis of the first-strand cDNA from the extracted total RNA was conducted using a QuantaBio qScript cDNA synthesis kit (VWR Canada, Edmonton, AB). Primers and gBlocks were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Triton X) containing a cocktail of protease and phosphatase inhibitors using a bead disruptor (Omni, VWR). Homogenates were centrifuged at 2100g for 10 min at 4°C and supernatants stored at −80°C ...
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bioRxiv - Molecular Biology 2019Quote: ... and grown to saturation overnight in an incubating microplate shaker (VWR 12620-930) (VWR International, Radnor, PA) at 30°C ...
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bioRxiv - Immunology 2021Quote: ... The absorbance spectra of the fluorochromes were preliminarily measured with SpectraMax M5 microplate reader (VWR, Radnor, PA) using 1:100 dilution in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... 10,000 viable cells were plated per well in white polystyrene 384-well microplates (Greiner Bio-One; VWR). Cells were exposed to MRX-2843 and venetoclax (5 – 1,000 nM ...
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bioRxiv - Bioengineering 2023Quote: ... deposited 10,000 viable cells per well (0.25×106/mL) in 384-well microplates (Greiner Bio-One; VWR). Cells were combined with drug matrices using a Beckman NX Liquid Handler (Beckman Coulter ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated using peqGOLD Trifast (VWR) or miRNeasy micro kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... Alkaline phosphatase coupled secondary antibodies were used for all blots followed by direct detection with NBT/BCIP (VWR) substrates on PVDF membranes.
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bioRxiv - Neuroscience 2024Quote: ... We collected the lysates from the wells using RIPA buffer (with phosphatase and protease inhibitors from Pierce VWR) and scraping the wells ...
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bioRxiv - Plant Biology 2020Quote: ... The small diameter tube outlets were placed into a 96-well microplate cover (VWR International, LLC, PA, USA) with holes drilled in a pattern to match the 96-well collection plate ...
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bioRxiv - Neuroscience 2023Quote: ... All washing and incubation steps during the staining procedure were performed while shaking (300 rpm, microplate shaker, VWR). Free-floating sections were washed (three times in 1xPBS for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... All PKC assays (except the PKC-mu and the PKC-nu assay) additionally contained 1 mM CaCl2 (VWR; Cat. # 1.02382), 4 mM EDTA (Roth ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted with peqGOLD TriFast Reagent (VWR). First-strand cDNA synthesis was performed from 1 µg RNA using M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... from pre-cultures were taken by a Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader (VWR, cat # 89429-536). Pre- cultures were diluted to a starting OD600 of 0.02 in 5 mL 0.1x R2A ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... The samples were then kept at 55°C for 30 minutes in a shaker (Incubating microplate shaker, VWR, The Netherlands), 20% of formic acid (5 µL ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from cells using peqGOLD TriFast (VWR) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total RNA from MEFs was isolated using peqGold Trifast (VWR) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... fish were acclimatized for 4–5 days prior to the assay in a cylindrical assay chamber (325 mL glass dish, 10 cm × 5 cm, VWR, Radnor, PA, USA) filled with conditioned water (pH 6.8–7.0 ...
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bioRxiv - Microbiology 2019Quote: ... Growth in the phylloplane was assayed by re-isolating Protomyces from inoculated leaves by placing leaves in 2 ml Eppendorf tubes with 1 ml 0.025% Silwet-L77 in water and shaking (800 rpm; VWR microplate shaker) for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Plaque assays were fixed using formaldehyde (VWR, Leighton Buzzard, UK) and stained using 0.1% Toluidine Blue (Sigma-Aldrich) ...
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bioRxiv - Physiology 2023Quote: ... Mouse heart tissue was homogenized in ice-cold 1X RIPA buffer supplemented with 1X protease inhibitors and 1X Phosstop phosphatase inhibitor using a bead mill homogenizer (VWR, #75840–022). Homogenates were sonicated for 10sec ...
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bioRxiv - Plant Biology 2020Quote: ... Total chlorophyll was extracted with dimethyl sulfoxide solvent (VWR, Radnor, PA), and measured using a SmartSpec™ Plus spectrophotometer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The total cell count determined by hemocytometer with trypan blue (VWR). For each sample 1.5 x 106 cells first underwent Fc receptor blockade with rat anti-mouse FcγRIII/II receptor (CD16/32 ...
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bioRxiv - Physiology 2019Quote: Total RNA was extracted using peqGOLD TriFast reagent (VWR, Dresden, Germany). Complementary DNA (cDNA ...
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bioRxiv - Bioengineering 2024Quote: ... These liver samples were homogenized with RIPA lysis buffer premixed with 1X protease and phosphatase-inhibitor cocktail and 1x EDTA (to prevent non-specific protease, metalloprotease, or phosphatase-mediated digestion) using a benchtop homogenizer (VWR VDI 12 Homogenizer). Samples were centrifuged at 4000 rpm for 10 minutes at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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Panacea: a hyperpromiscuous antitoxin protein domain for the neutralisation of diverse toxin domainsbioRxiv - Microbiology 2021Quote: Toxicity-neutralisation assays were performed on LB medium (Lennox) plates (VWR). E ...
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bioRxiv - Microbiology 2019Quote: Toxicity-neutralisation assays were performed on LB medium (Lennox) plates (VWR). E ...
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bioRxiv - Pathology 2021Quote: ... the assay buffer was 1% bovine serum albumin (VWR Chemicals, UK) in Tris Buffered Saline (TBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Assay was conducted on a black walled 96-well petriplate (VWR) according to manufacturer’s instructions and fluorescence was measured on an Agilent BioTek Microplate Reader (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... and reverse transcription (RT)-qPCR assays were done using qScript (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (VWR Life Science) supplemented with phosphatase inhibitor cocktail 2 (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Cell Biology 2022Quote: ... cAMP was measured using Cayman ELISA cAMP assay (VWR, Cat #75817-364) following manufacturer recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was prepared from these cells using RiboZol reagent (VWR Life Science) followed by RNeasy Mini kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: Total RNA from BMDM was isolated (peqGOLD, 13-6834-02, VWR Life Science) and reverse transcribed (PrimeScript RT reagent kit ...
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bioRxiv - Microbiology 2023Quote: ... the total combined biomass for each plate was harvested using cell scrapers (VWR) and suspended in 300 µL DNase-free water ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from cells with peqGOLD TriFast (VWR, cat.no. 30-2010). Synthesis of cDNA was performed with M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Microbiology 2019Quote: ... A total of 1 x 108 CFU was added to fresh sheep blood (VWR) in V-bottom 96-well plates (Corning) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from muscles using Ribozol (Cat. No CA97064-950, VWR international) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Lipid uptake was measured using the QBT Fatty Acid Uptake Assay (VWR, 10048-826) using the BioTek Synergy plate reader ...
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bioRxiv - Microbiology 2022Quote: Biofilm formation assays were performed in 96 well flat-bottomed plates (VWR 10861-562) coated with 100uL of BSA or Fg (150 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration throughout the study was determined via Bradford assay (catalogue number-VWR M172).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Plant Biology 2020Quote: All germination assays were performed in sterile 24-well culture plates (VWR International; 734-2779), using 500 μL of the specified solution and dry seeds (typically 20 ...
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bioRxiv - Cell Biology 2021Quote: ... a competitive ELISA cAMP assay was used (Enzo Cat #581001 or VWR Cat #75817-364). Cells expressing bPAC constructs were plated on 6-well plates ...
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bioRxiv - Microbiology 2023Quote: ... The assay plates were then sealed using a sterile polyester film (VWR, cat # 89134-432) before placing the lid to prevent the panel from dehydrating during incubation ...
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bioRxiv - Biochemistry 2024Quote: In vitro assays were performed using NUNC 96-well plates (VWR International GMBH, Darmstadt, Germany) and a CLARIOstar plate reader (BMG Labtech ...