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Citations for VWR :
1 - 50 of 429 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Resistance selection using fixed concentrations of COV-siRNA1 or COV-siRNA1+2 was conducted in Vero E6 cells cultured in DMEM supplemented with 10% FBS (VWR) and 1x Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: Plant or mammalian produced SARS-CoV-2 RBD protein were diluted in sterile 1X PBS to 2 μg/mL and coated onto a 96-well plates (VWR #62402-959) (50 μL /well ...
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bioRxiv - Genetics 2021Quote: ... anti V5-tag (VWR, MEDMMM-) and Anti-pgk1(Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells were plated onto 12-mm glass coverslips (VWR) in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were plated on sterilised 24 mm coverslips (VWR) at a low density to allow for single cells to be measured and clear background regions were available to record ...
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bioRxiv - Molecular Biology 2022Quote: HCT116 and HEK293 cells were seeded on 1.5mm coverslips (VWR) 24 hours prior to transfection with pLEICS25 wild-type or mutant GFP-Sam68 ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... infectivity and spread cells were stained with a SARS-CoV-2 anti-N rabbit polyclonal antibody for 1 hour followed by 594-conjugated secondary Goat anti-Rabbit IgG (VWR, GtxRb-003-D594NHSX) for 1 hour ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Bioengineering 2024Quote: HEK293 cells were seeded at 180,000 cells per well in a 24-well tissue culture plate (VWR) containing 500 µl cell culture media 24 hours prior to transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Microbiology 2024Quote: Cells from bone marrow of 6-10-week-old male or female wild-type or Myd88-/- Ticam-/- Mavs-/- (49) C57BL/6 mice (see Table S1) were cultivated in RPMI 1640 (VWR) with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Neuroscience 2022Quote: ... or 2% BSA (2% Bovine Serum Albumin (VWR), 50mM Tris-Cl ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Genetics 2019Quote: ... Eluates were analysed by SDS-PAGE followed by western blotting with antibodies anti-V5 tag (VWR, MEDMMM-0168-P), anti-Pgk1 (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... 2% FBS (VWR), doxycycline and ROCK inhibitor ...
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bioRxiv - Genomics 2020Quote: ... 2 mM CaCl2 (VWR International Ltd ...
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bioRxiv - Biochemistry 2023Quote: ... 2% maltose monohydrate (VWR), or 5% glycogen from oyster (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM glutamine (VWR), 100 U/ml of penicillin ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... 2% dextrose (VWR #90000–904)) and grew populations at 30°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2% dextrose (VWR #90000–904)) with ampicillin sodium salt (100ug/mL (VWR #97061-440)) ...
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bioRxiv - Genomics 2020Quote: ... and 2% dextrose (VWR # BDH0230). BMGY medium contained 10 g /l (1% (w/v) ...
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bioRxiv - Genomics 2020Quote: ... 2% peptone (VWR #90000-264) and 2% dextrose (VWR # BDH0230) ...
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bioRxiv - Systems Biology 2020Quote: ... contained 2%-agar (VWR Chemicals), Yeast Extract and Peptone (YEP ...
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bioRxiv - Microbiology 2021Quote: ... and 2% FBS (VWR, #104B16). The plates were then incubated with either ACE2-Fc (Sino biological ...
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bioRxiv - Cancer Biology 2022Quote: ... and 2 mM glutamine (VWR). Cells were then overlaid with fresh media and sequentially subjected to 1 μM oligomycin and then concomitant 1 μM rotenone and antimycin A ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2% dextrose (VWR #90000– 904), 5 g/L Bacto casamino acids (VWR #223050) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2% dextrose (VWR #90000–904), 5 g/L Bacto casamino acids (VWR #223050) ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 2% FBS (VWR) and 1% penicillin-streptomycin (Gemini Bio-products ...
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bioRxiv - Microbiology 2019Quote: ... 2 mM L-glutamine (VWR), and 100 IU/ml penicillin – 100 µg/ml streptomycin (VWR) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2% dextrose (VWR #90000–904), 5 g/L Bacto casamino acids (VWR #223050) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2% dextrose (VWR #90000–904)) with 150 uL glycerol stock and rocking at 30°C for 12-24 hr ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 2% dextrose (VWR #90000–904), 5 g/L Bacto casamino acids (VWR #223050) ...
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bioRxiv - Immunology 2023Quote: ... 2 glass coverslips (VWR: 48382085) were sonicated for ∼5 minutes in nanopure water (18.2 MΩ ...
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bioRxiv - Microbiology 2023Quote: ... L-glutamine (2 mM, VWR), streptomycin (100 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... CuSO4 (VWR VW 3312–2), Fe(NO3)3·9H2O (Sigma F–1143) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM L-glutamine (VWR), and 100 IU/mL penicillin – 100 µg/mL streptomycin (VWR) ...
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bioRxiv - Physiology 2023Quote: ... followed by 2 x 2-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...