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Citations for VWR :
1 - 50 of 947 citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Molecular Biology 2020Quote: ... zebrafish embryos were homogenized in 1 mL PBS and shaken in a glass vial (VWR) with 1 mL methanol and 2 mL chloroform containing internal standards (13C16-palmitic acid ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Neuroscience 2022Quote: ... Zebrafish embryos and larvae were maintained in a non-CO2 incubator (VWR) at 28°C on the same light-dark cycle as the aquatic facility.
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Genetics 2021Quote: ... anti V5-tag (VWR, MEDMMM-) and Anti-pgk1(Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG-tag(VWR# 95040-926), and Streptavidin-HRP(JacksonImmuno ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Neuroscience 2024Quote: ... Zebrafish embryos and larvae were maintained in embryo medium in a non-CO2 incubator (VWR) at 28°C on the same light–dark cycle as the aquatic facility ...
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bioRxiv - Cancer Biology 2024Quote: Zebrafish were euthanized according to IACUC guidelines and fixed in 10% neutral Buffered Formalin (VWR) for 24 hours at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Genetics 2023Quote: ... compound (VWR, Batavia, IL) and frozen in a bath of 2-methylbutane cooled with liquid nitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Rpoβ (anti-RNA polymerase beta mouse monoclonal antibody [clone: 8RB13]; VWR) was diluted 1:20,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Neuroscience 2020Quote: ... Individual zebrafish larvae were placed in 96-well plate format using Whatman UNIPlate square 96-well plates (VWR, 13503-152). In order to produce a scotopic stimulus ...
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bioRxiv - Biophysics 2024Quote: ... Immuno-labeled proteins were detected using a chemiluminescence detection system (Pierce Biotechnology; Rockford, IL, USA) on Kodak Biomax film (VWR Scientific; Radnor, PA, USA).
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bioRxiv - Cell Biology 2024Quote: ... followed by resuspension in culture medium (DMEM low glucose, 10% HI-FBS, 1% Penicillin/Streptomycin) and filtered through a 40 μm cell strainer (VWR, USA). The obtained single cell suspensions of ovarian primary cells were seeded either for monolayer 2D culture (1.5 × 106 cells/well ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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Pleiotropy in FOXC1-attributable phenotypes involves altered ciliation and cilia-dependent signalingbioRxiv - Genetics 2020Quote: ... embedded in Clear Frozen Section Compound (VWR, Richmond, IL) and frozen in a dry ice bath ...
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bioRxiv - Immunology 2024Quote: ... Pan-flavivirus E protein antibody (1:100, VWR Enzo, 76285-044), GFAP (1:250 ...
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bioRxiv - Bioengineering 2024Quote: ... and 20 ng/ml recombinant mouse GM-CSF (VWR, Radnor, PA) (DC media) ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was blocked with 5% bovine serum albumin (VWR, Batavia, IL) in PBS containing 0.1% Tween-20 for one hour at room temperature and then incubated in primary antibodies overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Cell Biology 2020Quote: ... Pipettes were manufactured by pulling 100 μL capillary tubes (VWR, Arlington Heights, IL) to a tip diameter of ~0.5 micron (tip resistance = 1-5 MΩ ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were dried in an oven (INCU Line ILS 6, VWR, Radnor, PA, USA) at 50°C over 5 days and size sorted with a vibratory sieve shaker (AS 300 control ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... 5 × 105 cells were cytospun onto 22 × 60 mm #1.5 coverslips (VWR international, Batavia, IL), pre-coated with CellTak (Corning) ...
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bioRxiv - Neuroscience 2023Quote: ... on a Leica cryostat and directly transferred to slides (Superfrost Plus; VWR International, Arlington, IL). Sections of brains ...
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bioRxiv - Biochemistry 2023Quote: ... at which point protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, #437145X) at the final concentration of 0.5 mM ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Bioengineering 2024Quote: Pre-cleaned conditioned media (700 x g for 5 min and 2000 x g for 10 min) from HeLa-WT and HeLa-CD81-Snorkel-tag overexpressing cells was sterile filtered using syringe (VWR) with cellulose acetate membrane filters (0.22 µm pore size ...
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bioRxiv - Bioengineering 2021Quote: ... The solution was then transferred into 12-14 kDa MWCO dialysis membranes (VWR, Chicago, IL, USA) and dialyzed against DI water for one week ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...