Labshake search
Citations for VWR :
401 - 450 of 1130 citations for Recombinant Mouse Tissue Inhibitor Of Metalloproteinase 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... 2-2.5 × 106 frozen or freshly isolated monocytes were seeded in 10 cm non-tissue culture treated dishes (VWR, #734-2796) in 10 mL RPMI 1640 (Biowest ...
-
bioRxiv - Genomics 2022Quote: Colonic tubes were dissected within minutes of sacrificing mice and tissues dried from excess fluids and embedded in Optimal Cutting Temperature (OCT, Fisher Healthcare, USA) in large molds (VWR, USA) pre-filled with OCT ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
-
bioRxiv - Plant Biology 2023Quote: ... Apogee grain were left for 2 days at 5°C in water for imbibition before being placed individually onto cotton in a 24-well tissue culture plate (VWR, USA) and left to germinate for 3 days under high humidity conditions (<90% relative humidity ...
-
bioRxiv - Neuroscience 2023Quote: ... spinal cords were incubated overnight with 50% Optimal Freezing Temperature (OCT) (Tissue-Tek, Cat No. 25608-930, VWR, Radnor, PA, USA) medium and 50% 20%-sucrose dissolved in PBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Tissues were sectioned onto gold slides (see below for more information) for MIBI analysis and SuperFrost glass slides (VWR, 48311-703) for GeoMx analysis ...
-
bioRxiv - Developmental Biology 2021Quote: ... 50mM Tris-HCl pH 8.1,5mM EDTA) containing protease inhibitors (Pierce mini tablets EDTA-free, VWR, Radnor, PA, Cat #PIA32955) to obtain nuclear extracts ...
-
bioRxiv - Developmental Biology 2024Quote: ... which were adjusted to a concentration of 1.25 × 106 cells/mL in iPS Brew + 10 µM ROCK inhibitor (Y-27632, VWR). 800 µL of cell suspension (approximately 1 million cells ...
-
bioRxiv - Microbiology 2020Quote: ... Four-micron sections of formalin-fixed paraffin-embedded tissues were mounted on positively charged Superfrost® Plus Slides (VWR, Radnor, PA, USA). The RNAscope® ISH assay was performed using the RNAscope 2.5 HD Red Detection Kit (ACD ...
-
bioRxiv - Cell Biology 2020Quote: Gastrocnemii were homogenized with 25 strokes using a 5 mL PTFE tissue grinder with clearance 0.15-0.25 mm (VWR 89026-392, 89026-404) at 3,000 rpm in RIPA lysis buffer (VWR 97063-270 ...
-
bioRxiv - Cell Biology 2019Quote: ... Fetal liver was dissected from the embryo and embedded in a 25-mm x 20-mm x 5-mm Tissue-Tek Cryomold (VWR, 25608-916) with optimal cutting temperature compound (Tissue-Tek O.C.T. ...
-
bioRxiv - Cancer Biology 2019Quote: ... in RPMI with 5% FBS and 1% penicillin/streptomycin for 20 minutes in a humidified incubator at 37°C Tissues were washed with PBS and further dissociated in 0.25% Trypsin (VWR, Cat. # VWRL0154-0100) for 30 minutes in the incubator ...
-
bioRxiv - Bioengineering 2020Quote: ... The tissue was sectioned using 40- to 60-μm slices and transferred onto a Superfrost Plus glass slide (VWR Inc., #48311-703). Sections were stored at −20°C before immunostaining.
-
bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
-
bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...
-
bioRxiv - Molecular Biology 2020Quote: Adult hearts were dissected from Msk1/2 KO mice and covered in a layer of Tissue-Tek optimum cutting temperature (OCT) in Tissue-Tek® Cryomold® Molds (15×15×15 mm) and flash-frozen in liquid nitrogen-cooled isopentane (VWR). 10 μm ventricular sections were cut using a Leica cryostat and attached to SuperFrost Plus™ slides (VWR) ...
-
bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
-
bioRxiv - Physiology 2022Quote: ... frozen and crushed in liquid nitrogen for protein and RNA extraction or ii) embedded in Tissue-Tek OCT compound (VWR, Mississauga, Canada) and frozen in isopentane cooled with liquid nitrogen for cryostat sectioning54 or iii ...
-
bioRxiv - Cell Biology 2019Quote: ... 15 μl of tissue suspension was pipetted onto a well of a 10-well PTFE diagnostic slide (631-1371, VWR International GmbH) and left to dry completely ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue was mechanically dissociated by gentle up and down pipetting using a 200 μl siliconized pipette tip (VWR cat No. 53503-794). The resulting suspension was seeded in small 20 - 50 μl droplets on ibiTreat μ-Dishes (Ibidi cat No ...
-
bioRxiv - Cell Biology 2022Quote: ... Animals were then homogenized 20x in 3 mL of M9 buffer using an ice-cold 15 mL Dura-Grind Stainless Steel Dounce Tissue Grinder (VWR, 62400-686). Crude lysate was transferred to 1.5 mL tubes and frozen in liquid N2.
-
bioRxiv - Microbiology 2022Quote: ... Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™ ...
-
bioRxiv - Molecular Biology 2023Quote: ... Worms were resuspended in 7 ml pre-chilled Nuclei Purification Buffer+ and dounced on ice in a Wheaton dura-grind stainless steel dounce tissue grinder (VWR 62400-675) for 12 strokes ...
-
bioRxiv - Genetics 2023Quote: ... Small pieces of cotton wool soaked with sterile water were placed in each well of a 24-well tissue culture plate (VWR International, US). One wheat seed was placed with the crease facing downwards into each well ...
-
bioRxiv - Genomics 2023Quote: ... and their kidneys and brains were quickly collected and frozen immediately in optimal cutting temperature compound (Tissue-Tek O.C.T.; VWR, cat. no. 25608–930), before storing at −80 °C ...
-
bioRxiv - Cell Biology 2023Quote: Lyophilized liver samples were homogenized in 10 mM phosphate buffer at pH 6 (28 mg tissue per ml buffer) on a bead beater (VWR, Radnor, PA) for 1 min ...
-
bioRxiv - Cell Biology 2023Quote: ... donkey anti-mouse IgG conjugated to IRDye 800CW (VWR, cat. no. 102673-332) or goat anti-rabbit IgG conjugated to IRDye 680RD (VWR ...
-
bioRxiv - Molecular Biology 2023Quote: ... Nitrocellulose membrane was then incubated with goat Anti-mouse (102971-154, VWR International) and goat Anti-rabbit (103011-498 ...
-
bioRxiv - Immunology 2024Quote: ... Mouse tumor sections were mounted on microscope cover glasses (#631-0170, VWR, France) using Fluoromount-G (#00-4958-02 ...
-
bioRxiv - Microbiology 2021Quote: Antibiotic tolerance assays using co-cultures were performed using a membrane-separated 12-well tissue plate cultures containing 0.1 μm pore PET membranes (VWR, Cat. No. 10769–226), following previously protocols (Meirelles et al. ...
-
bioRxiv - Immunology 2022Quote: ... PBMCs (4×105 cells/well) were laid in sterile round bottom 96-well tissue culture treated plates (VWR, The Netherlands, cat #734-2184) in Dutch modified RPMI 1640 medium containing 50 µg/mL Gentamicine ...
-
bioRxiv - Physiology 2023Quote: ... Each brain tissue was carefully collected from the cranium individually and snap-frozen in a 1.5 mL microcentrifuge tube (VWR International, Radnor, PA, USA) chilled with liquid nitrogen ...
-
bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium ...
-
bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
-
bioRxiv - Cell Biology 2023Quote: Mouse skin and wound beds were embedded in optimum cutting temperature (OCT) compound (VWR) and wound beds were sectioned through their entirety to identify the center ...
-
bioRxiv - Microbiology 2020Quote: ... co-culture experiments were performed using membrane-separated 12-well tissue plate cultures containing 0.1 µm pore PET membranes (VWR® Cat. No. 10769-226). Briefly ...
-
bioRxiv - Bioengineering 2020Quote: ... The tissue was cut into 1mm pieces and placed in cryomolds before being left to incubate in 361603E OCT (VWR International, Radnor, PA, USA) for 30 minutes ...
-
bioRxiv - Genomics 2023Quote: ... (Lexogen SPLIT RNA kit) was added to each tube and the tissue homogenized using a plastic pestle (Kontes Pellet Pestle, VWR, catalogue number: KT749521-1500). Samples remained on ice to maintain RNA integrity while other samples were homogenized ...
-
bioRxiv - Molecular Biology 2023Quote: Mouse and chicken brains were dissected and immediately immersed in OCT embedding medium (361603E, VWR) for a washing step ...
-
bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated donkey antirabbit (NA934V) and anti-mouse (NA931-1M (both from VWR International Ltd.) secondary antibodies were used at 1:3000-5000 ...
-
bioRxiv - Bioengineering 2021Quote: Fresh frozen lung lobe tissues with highest injury score (n=9) were homogenized using VWR® Mini Bead Mill Homogenizer (VWR International LLC., Radnor, PA) following manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2020Quote: 1-Aminocyclopropane-1-carboxylic acid (ACC; VWR P10007) was dissolved in sterile water (10 mM stock ...
-
bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 1 (VWR) were cleaned by a 20 minute exposure in a 2.6 L Zepto plasma laboratory unit (Diener Electronic) ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse brains were first fixed in 4% paraformaldehyde (PFA) in PBS solution (VWR, Cat# ALFAJ61899.AK) for 24 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Matrigel Basement Membrane Matrix (1:1) (354234, VWR).
-
bioRxiv - Bioengineering 2024Quote: ... These liver samples were homogenized with RIPA lysis buffer premixed with 1X protease and phosphatase-inhibitor cocktail and 1x EDTA (to prevent non-specific protease, metalloprotease, or phosphatase-mediated digestion) using a benchtop homogenizer (VWR VDI 12 Homogenizer). Samples were centrifuged at 4000 rpm for 10 minutes at 4 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-S100 1:300 (VWR/ProteinTech 15146-1-AP), Chick anti-Neurofilament 200 kDa 1:500 (Aves NFH) ...