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Citations for VWR :
51 - 100 of 145 citations for Recombinant Human SLAMF1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 20% trichloroacetic acid (TCA – VWR) on ice overnight at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Physiology 2021Quote: 6xHis fusion proteins were grown in M15 cells (VWR) and prepared as above except that after IPTG induction ...
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bioRxiv - Biochemistry 2023Quote: ... Staining was carried out with InstantBlue Protein Stain (VWR).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... DAOY human medulloblastoma cell line was obtained from ATCC and was cultured in EMEM (VWR) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... 10^5 human SHSY-5Y cells seeded on 18 mm #1 round cover glass (VWR) in a 12 well culture plate ...
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bioRxiv - Neuroscience 2020Quote: Snap frozen human DRGs were sectioned onto superfrost plus glass slides (VWR International, Leuven, Belgium) at 10μm thickness ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips ...
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bioRxiv - Genomics 2023Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR 25608-930) and sectioned on a Leica cryostat at -17C at 10 μm onto Vizgen MERSCOPE coverslips ...
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bioRxiv - Molecular Biology 2021Quote: ... The elutes were incubated with S-protein agarose (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...
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bioRxiv - Cell Biology 2019Quote: ... 0.45μm low protein binding membrane (#28145-479) was from VWR International ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined with Bradford reagent (VWR, E530-1L). Western blotting was performed as previously described (43 ...
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bioRxiv - Immunology 2023Quote: ... Thereafter proteins were recovered using 10 kDa spin filters (VWR), and samples were reduced ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a 0.45 µm nitrocellulose membrane (VWR), by electroblot ...
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bioRxiv - Molecular Biology 2021Quote: ... Both Jurkat and primary human T cells were treated with 100 μg ml-1 cycloheximide (VWR) 5 minutes before harvesting ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin embedded human trephine and tonsil tissues were sliced on to Superfrost Plus® slides (VWR) and incubated at 60°C for 45min ...
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bioRxiv - Biophysics 2021Quote: Peripheral blood monocytes were isolated from human blood using 15 Ml Ficoll-Paque (VWR, Darmstadt, Germany) centrifugation gradient ...
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bioRxiv - Microbiology 2019Quote: The human monocyte cell line U937 (ATCC CRL-1593.2) was cultured in RPMI 1640 media (VWR) supplemented with 10% FBS at 37C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Human neuroblastoma SH-SY5Y cells (ATCC, CRL-2266) were cultured in DMEM medium (VWR, 0101–0500) supplemented with 10% FBS (Gemini Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and human keratinocyte growth supplement (HKGS) 100X (S-001–5) from Cascade Biologist were procured from VWR Corporation (Missouri ...
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bioRxiv - Plant Biology 2020Quote: ... and all protein estimations were done using Bradford reagent (VWR Inc.).
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bioRxiv - Microbiology 2020Quote: ... Protein syntheses were induced by 2% (wt/vol) galactose (VWR International) at 30 °C for 4 h at 250 rpm when the OD600 reached 0.8–1.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to a PVDF membrane (VWR, Cytiva, #10061-492), blocked with 5% powdered milk in TBST (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% Rho-associated protein kinase (ROCK) inhibitor (Y-27632; VWR). Each EHT construct consisted of 1.0 x106 cells ...
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bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1 mg/mL bovine serum albumin protein (VWR, 97062-508), then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred to nitrocellulose blotting membranes (10-6000-01, VWR) in Harlow buffer at 350mV and 4°C for 2 hours ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Separated proteins were transferred onto 0.45 μm nitrocellulose transfer membranes (VWR International). Protein loading and transfer quality were checked by staining with Ponceau S ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was isolated using Ni-NTA Resin (VWR Cat. No. 786-940) and eluted with 2 mL of elution buffer (300 mM NaCl ...
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bioRxiv - Microbiology 2019Quote: ... Insoluble protein pellets were resolubilized in 3 M urea (VWR, Radnor, PA) and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
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bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), mouse anti-NeuN (Sigma # MAB377 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-S100 Beta (VWR/Protein Tech #15146-1-AP, 1:300), rabbit anti-dsRed (Takara #632496 ...
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bioRxiv - Biochemistry 2019Quote: ... spectra of native, human BACE1 (6.12 μM, 30 μl; Acro Biosystems, USA) in 50 mM sodium acetate (pH 4.0; VWR, UK) was obtained using a J-1500 Jasco CD spectrometer and Spectral Manager II software ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Human foreskin fibroblasts (HFF, kindly provided by Denise Galloway) were maintained in Minimum Essential Media-α (MEM-α; VWR) supplemented with 20% FBS (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
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bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...
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bioRxiv - Microbiology 2019Quote: ... strains [69], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
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bioRxiv - Microbiology 2023Quote: Toxoplasma strain TATi-RHΔku80 [41] was cultured in human foreskin fibroblasts in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA, USA) supplemented with 10% fetal bovine serum (VWR) ...