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Citations for VWR :
51 - 100 of 196 citations for Recombinant Human ROR1 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The MES stock was filter sterilized using a 0.22 µm PES filter (VWR, Cat. No. 73520-982). The 1000X vitamin mix stock was prepared in water using the recipe outlined in Table S1B and filter sterilized with a 0.22 µm MCE filter ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Molecular Biology 2024Quote: ... 55 ng/ml dexamethasone (R&D Systems, distributed by VWR; VWR cat #: 1000505-728), 10 ng/ml human basic fibroblastic growth factor (BioPioneer ...
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bioRxiv - Molecular Biology 2024Quote: ... 55 ng/ml dexamethasone (R&D Systems, distributed by VWR; VWR cat #: 1000505-728), 10 ng/ml human basic fibroblastic growth factor (BioPioneer ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Cell Biology 2020Quote: ... 2900 rpm 10 min and 3500 20 min) and passed through a 0.22 µm PES membrane filter (VWR). To enrich EV concentration we used the Total Exosome Isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... Lentiviral-containing supernatants were harvested two days post-transfection and passed through a 0.45µm PES syringe filter (VWR). Ligand-expressing 293T cells were generated by transducing 293T cells (100,000 per well in a 12-well dish ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged for 15 min at 14,000 × g through 10 kDa molecular weight cut-off filters (modified PES, VWR), and transferred into HPLC vials.
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was pooled together with the sucrose fraction and filtered through a 0.22 μm PES membrane (VWR) and used immediately or stored at 4°C for further purification steps.
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bioRxiv - Plant Biology 2024Quote: ... the collected culture medium was filtered with a 0.45 μm polyethersulfone (PES) membrane (VWR International, Radnor, PA, USA) to avoid contamination by the cellular proteins ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Plant Biology 2022Quote: ... The phosphoproteome samples were resolubilized in 50 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was also transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Plant Biology 2022Quote: ... The reference proteome sample was resolubilized in 30 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was transferred to HPLC vials and injected into the LC-MS/MS instrument.
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Tryptic peptides were resolubilized in 30 μl of 0.05% TFA and 2% ACN in H2O filtered through 10 kDa MWCO PES membrane spin filters (VWR) at 14,000×g for 15 min and transferred to HPLC vials.
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Plant Biology 2021Quote: ... was kept at 30°C during the injections and all eluents were filtered through 0.2 μm PES filter (VWR, USA) and stored under helium ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentivirus-containing medium was harvested 72 h post-transfection by filtration through a 0.45 μm polyethersulfone (PES) filter (VWR Sciences). The filtered lentivirus was aliquoted ...
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bioRxiv - Microbiology 2023Quote: ... thetaiotaomicron in WC for 48 h without mucin beads and sterilized the spent medium through a filtration with 0.2-μm sterile syringe filters (PES; VWR International). In addition ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The samples were purchased between winter 2020 and spring 2021 and transported to the laboratory in polyethylene (PE) bags (VWR). One FCA ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
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bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Microbiology 2021Quote: ... and Zn were analyzed for a subset of samples after filtration using syringe filter (0.45 µm, PES, VWR International, Darmstadt, Germany). The LOQs of the dissolved Cr ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant media containing viral particles was harvested and filtered through a 0.45 µm polyethersulfone (PES) filter (VWR, Randor, Pennsylvania, US) and aliquoted ...
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bioRxiv - Microbiology 2021Quote: ... Cultures were collected by spinning 25 mL of culture in a tabletop centrifuge (VWR symphony 4417 R) at 2000 x g for 2 min with a swinging bucket rotor fitting 50-mL conical tubes ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Neuroscience 2024Quote: Detection with 3,3′-diaminobenzidine (DAB) was performed using horseradish peroxidase labeled secondary antibodies and DAB Quanto chromogen and substrate (VWR, Radnor, PA).
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bioRxiv - Biophysics 2022Quote: ... as diluent and were filtered through a 0.1 µm PES filter mounted onto a 50 mL centrifuge tube (#514-0366, VWR, Radnor, PA, USA) before use ...
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bioRxiv - Microbiology 2022Quote: ... SigmaAldrich), and riboflavin (R4500, SigmaAldrich) were first sterile filtered (0.2 um, Media bottle filtration unit with PES membrane, VWR No. 514-0297) and added to the media prior to each experiment and the pH was adjusted to 6.7 with NaOH immediately before the start of the experiment.
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bioRxiv - Cancer Biology 2023Quote: ... NAC-containing water was prepared using reverse osmosis filtered tap water and granulated N-acetyl-L-cysteine (>99%, MilliporeSigma Canada, Oakville ON) and was sterilized using 0.2 um PES filter units (VWR Canada, Mississauga ON). The prepared water was stored for a maximum of 2 months before use ...
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bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...