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Citations for VWR :
1 - 50 of 77 citations for Recombinant Human Insulin Receptor Isoform Short His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Biophysics 2020Quote: ... Embryos were dechorionated by short immersion in 7% sodium hypochlorite solution (VWR). After extensive rinsing with water ...
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bioRxiv - Biophysics 2023Quote: ... Embryos were dechorionated by short immersion in 7% sodium hypochlorite solution (VWR). After extensive rinsing with water ...
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bioRxiv - Immunology 2021Quote: ... and lysed using 29G insulin syringes (VWR). Lysed cells were then stored at −80°C prior to RNA purification ...
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bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... insulin (cell culture grade) (VWR Cat# 47743-634), and 0.01 nM dihydrotestosterone (DHT).
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered into 2 mL short thread vials with crimp caps (VWR International GmbH ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered into 2 mL short thread vials with crimp caps (VWR International GmbH ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and subsequently filtered into 1.5 mL short thread vials with crimp caps (VWR International GmbH ...
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bioRxiv - Immunology 2024Quote: ... the sera were treated with receptor-destroying enzyme (Denka Seiken, VWR), incubated overnight at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
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bioRxiv - Neuroscience 2021Quote: ... In short, free-floating sections were blocked (5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) and then incubated with different primary antibodies ...
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A bacterial signal transduction phosphorelay in the methanogenic archaeon Methanosarcina acetivoransbioRxiv - Microbiology 2021Quote: ... H2O up to a total volume of 10 μl was combined in a sealed short thread vial (VWR). For reducing condition freshly prepared DTT was added to a final concentration of 2 mM ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2019Quote: ... The samples were centrifuged again and 500 µl of the supernatant was transferred into short thread HPLC/GC vials (glass vial ND9, VWR, Germany) and sealed with short screw caps ...
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bioRxiv - Cell Biology 2019Quote: ... with one layer of Scotch double-sided tape on both short edges of the coverslips (VWR 22×40 mm, No. 1.5) as spacers to prevent excessive compression of the discs ...
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bioRxiv - Genetics 2023Quote: The muscarinic receptor antagonist atropine ((RS)-(8-Methyl-8-azabicyclo[3.2.1]oct-3-yl, VWR Cat. TCA0754-005G) was dissolved in ethanol and added to 1M (HS ...
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bioRxiv - Neuroscience 2020Quote: ... The dura beneath the craniotomy was removed using the tip of a 26g insulin syringe (VWR) and fine forceps (Fine Science Tools).
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bioRxiv - Genomics 2023Quote: ... injection of the test article was administered retro-orbitally using an insulin syringe (VWR, Radnor, PA). One day post-dosing ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Physiology 2024Quote: ... 1nM Insulin and 0.2% Fraction V fatty-acid free bovine serum albumin (BSA, E588, VWR, Radnor, PA). Cells were kept for 24-36 h.
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Developmental Biology 2022Quote: ... tjTS>lglRNAi ovaries (with no UAS-tagged fluorescence reporter) were dissected in Grace’s Insect Basal medium (VWR; #45000-476), and incubated in Dihydroethidium (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Intraperitoneal injection of 20ul of the Amp-101 into Dok-7KI/KI mice or saline into WT littermates was carried out on P4 using 0.3ml 30g insulin needles (VWR International Ltd). Mice were weighed every day from P3 until P30 after which they were weighed once per week.
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
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bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
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bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...