Labshake search
Citations for VWR :
1 - 50 of 194 citations for Recombinant Human CD19 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
-
bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
-
bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
-
bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
-
bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
-
bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
-
bioRxiv - Microbiology 2023Quote: ... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
-
bioRxiv - Immunology 2020Quote: ... recombinant β-lactamase (VWR) was added ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10 % FCS (VWR International, S181B) and 1 % Pen/Strep (Life Technologies ...
-
bioRxiv - Synthetic Biology 2023Quote: Recombinant murine thrombopoietin (TPO) (VWR, 10770-952).
-
bioRxiv - Neuroscience 2023Quote: ... supplemented with HEPES and 10% fetal calf serum (FCS) (VWR) 37°C/5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: ... with 10% fetal calf serum (FCS premium, S. Amer. Orig, VWR), 2 mM L-glutamine ...
-
bioRxiv - Bioengineering 2019Quote: Samples were filtered using 10K modified PES Centrifugal filters (VWR). Metabolites of remaining cell free supernatant were analyzed by HPLC ...
-
bioRxiv - Microbiology 2020Quote: ... The solution was filtered through 0.1 µm PES membrane (VWR) and stored in the dark at room temperature ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... All samples were transported to the laboratory in PE bags (VWR) and their polymer type was determined using Fourier-transformed infrared spectroscopy and differential scanning calorimetry in the case of HDPE and LDPE (see S.I ...
-
bioRxiv - Genomics 2020Quote: ... Lentivirus-containing media was filtered using 0.45 μM PES syringe filter (VWR) and mixed with polybrene (8 μg/ml) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... the supernatant was collected and filtered through a 0.45 PES filter (VWR).
-
bioRxiv - Microbiology 2021Quote: ... The viral supernatants were filtered through 0.45 μm polyethersulfone filters (PES, VWR), aliquoted and stored at −80°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Resulting solution was filtered through a centrifuge filter (30K PES membrane, VWR) for 7 min at 12,000 x g at 4°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Samples were purified with Polyethersulfone (PES) 10kDa MWCO filters (VWR, 516-0230P) and Sep-Pack Vac C18 1cc/50mgv columns (Waters ...
-
bioRxiv - Microbiology 2024Quote: ... The precipitated DNA was resuspended in TE buffer (VWR, Radnor, PE, USA) and purified using QIAamp DNA Mini Kit (Qiagen) ...
-
bioRxiv - Biochemistry 2024Quote: ... These media were filtered using 0.2 μm polyether sulfone (PES) filters (VWR) before use.
-
bioRxiv - Microbiology 2023Quote: ... Vero E6 (ATCC CRL- 1586) was cultured in DMEM supplemented with 5 % FCS (VWR) and 1 % penicillin−streptomycin−glutamine (PSG ...
-
bioRxiv - Genetics 2023Quote: ... Labeled oligos were purified using MicroSpin G-25 Columns (VWR, #95017-621), then scintillation counts were performed using Liquid Scintillation Analyzer Tri-Carb 4910 TR (Perkin Elmer) ...
-
bioRxiv - Genomics 2021Quote: ... and 5mL fractions were removed and filtered (70uM) into RPMI supplemented with 2% FCS (VWR) three times ...
-
bioRxiv - Developmental Biology 2022Quote: ... tjTS>lglRNAi ovaries (with no UAS-tagged fluorescence reporter) were dissected in Grace’s Insect Basal medium (VWR; #45000-476), and incubated in Dihydroethidium (Invitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... fluorescently labeled EV were purified using sepharose CL-2B (17-0140-01, VWR) size exclusion chromatography (SEC ...
-
bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and filtered through a 0.22 µM polyethersulfone (PES) membrane filter (VWR) and stored in aliquots at −80 ...
-
bioRxiv - Cell Biology 2020Quote: ... The cytosol fraction in the supernatant was concentrated using 10K Polyethersulfone (PES) membranes (VWR) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... EV suspensions were concentrated on 3 kDa polyethersulfone (PES) membrane centrifugal filters (VWR International) for 5 min at 14,000 rpm at 4°C (Sigma 3-KIS centrifuge) ...
-
bioRxiv - Immunology 2020Quote: ... washed with PBS containing 0.5% BSA Fraction V (VWR, Radnor, PE, USA; cat. 422371X) and incubated with anti-CD89 PE antibody for 30 min at 4 °C in the dark to fluorescently label Mono-Mac-6 cells ...
-
bioRxiv - Immunology 2020Quote: ... containing 0.5% bovine serum albumin (BSA) Fraction V (VWR, Radnor, PE, USA; cat. 422371X) and incubated with anti-CD89 PE antibody for 30 min at 4 °C in the dark to fluorescently label Mono-Mac-6 cells ...
-
bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... then centrifuged and the supernatant filtered through a 0.22 μm PES membrane (Avantor, VWR). For each clone a maximum growth rate was estimated from 5 measurements of optical density (OD ...
-
bioRxiv - Synthetic Biology 2023Quote: ... then plated onto YPM supplemented with 60 µg mL-1 5-fluorocytosine (5-FC) (TCF0321, VWR). Colonies that emerged were then transferred onto a new YPM 5-FC agar plate for clonal isolation ...
-
bioRxiv - Cancer Biology 2021Quote: ... The labeled samples were pooled together using a 10-µL glass syringe (VWR, Japan), starting always with the CP samples in each TMT set in order to minimize sample loss during the pooling.20 Samples were pooled into MS-sample vials with glass insert (TPX snap ring vial from Genetec ...
-
bioRxiv - Genomics 2022Quote: ... Cas9-EDV-containing supernatants were harvested and passed through a 0.45µm PES syringe filter (VWR) and concentrated with Lenti-X Concentrator (Takara Bio ...
-
bioRxiv - Physiology 2022Quote: ... a heat flared polyethylene tube (PE tube; VWR ID x OD: 0.023 x 0.038”, Radnor, USA) was inserted and tied into the anterior margin of the section ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
-
bioRxiv - Genomics 2022Quote: ... and supplemented with 10% (v/v) standard FBS (VWR or R&D Systems), 100 units/mL penicillin ...
-
bioRxiv - Developmental Biology 2023Quote: ... the same set of Axygen® FXF-384-XL-R-S tips (VWR) were used for the entire separation protocol and were rinsed with 5ul of G&T wash buffer at the end of the separation step ...
-
bioRxiv - Molecular Biology 2022Quote: ... We then transferred the column to a labeled DNA LoBind Eppendorf 1.5 mL tube (VWR # 022431021), added 20 uL nuclease-free H2O ...
-
bioRxiv - Plant Biology 2021Quote: ... The TSS media was filter-sterilized using a 0.2 μm polyethersulfone (PES) vacuum filter unit (VWR, USA) and adjusted the medium pH 7.0 using 10N NaOH ...
-
bioRxiv - Bioengineering 2022Quote: ... and controlled at 7.0 by addition of filter-sterilized 4.0 M KOH (0.2 µm PES filters) (VWR). The base vessel and 10-L feed vessels were connected via sterile 0.6-mm needles through septa on the head-plate (one feed vessel per reactor) ...
-
bioRxiv - Microbiology 2023Quote: ... The culture grown in CDM was sterile filtered with a 25 mm 0.22 µM PES filter (VWR) and transferred to a new test tube ...
-
bioRxiv - Microbiology 2023Quote: ... The MES stock was filter sterilized using a 0.22 µm PES filter (VWR, Cat. No. 73520-982). The 1000X vitamin mix stock was prepared in water using the recipe outlined in Table S1B and filter sterilized with a 0.22 µm MCE filter ...
-
bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 55 ng/ml dexamethasone (R&D Systems, distributed by VWR; VWR cat #: 1000505-728), 10 ng/ml human basic fibroblastic growth factor (BioPioneer ...