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Citations for VWR :
101 - 150 of 898 citations for Rat Segment polarity protein dishevelled homolog DVL 1 DVL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... all proteins were diluted in filtered (sterile syringe filters 0.45 μm cellulose acetate membrane, VWR International, Radnor, USA) Min buffer in the presence of either 0.5 mM ADP or ATP ...
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bioRxiv - Genetics 2020Quote: Quadriceps from P10 pups were dissected and total proteins extracted using radioimmunoprecipitation assay (RIPA) buffer (VWR, Radnor, PA) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... The sheared DNA was then pre-cleared using magnetic beads (Sera-Mag Protein A/G SpeedBeads, VWR 17152104010150) for 1 hour to remove non-specific binding ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Molecular Biology 2023Quote: ... then neutralized with 1 μL 5 M HCl (VWR BDH7419-1). The remainder of the library preparation was performed according to the xGen protocol except that post-ligation ...
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Evolutionary Biology 2020Quote: ... and then 1 hour in 1:1 ratio of 30% sucrose/0.1M phosphate buffer:OCT (VWR, Cat No. 25608-930). Tissue was embedded inside a mold made by the severed end of a 1.5mL microcentrifuge tube attached to a glass slide with nail polish and stored at −80°C until used ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared according to the manufacturer’s instructions and proteins were separated in 10 % Next Gel SDS-PAGE (VWR). Carbonylated proteins were detected with Immobilon Western Chemiluminescent HRP Substrate (MilliporeSigma).
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Immunology 2022Quote: ... The protein fraction was isolated by combining four times the volume of ice cold acetone (VWR Chemicals, PA, USA) to one volume of reconstituted PBMCsec ...
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bioRxiv - Systems Biology 2020Quote: ... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cultured in 150mm dish (Alkali scientific TDN0150) for protein experiment and in 6-well culture plate (VWR, 100062-892) for total RNA experiment and transfected at around 50-60% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... stably expressing enhanced green fluorescent protein (eGFP) were generated as described before[45] and cultured on 0.1% gelatin (VWR) pre-coated surfaces in MCDB-131 medium (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... culture medium was changed and lentivirus-containing supernatant was collected 48 hours after transfection and filtered through a 0.45 μm low-protein-binding filter (VWR). Target cells were plated in 6-well culture plates with a 60-80% confluency ...
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bioRxiv - Molecular Biology 2021Quote: ... and G418 sulfate (500 μg mL−1, from 50 mg mL−1 stock in H2O, from 712 U mg−1 powder, VWR). Cells were checked monthly for mycoplasma contamination by PCR assay ...
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bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
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bioRxiv - Biophysics 2020Quote: ... 1% (w/w) 10:1 (base/crosslinker w/w) Sylgard-184 (VWR) was added to the GEL-8100 and mixed well for 60 seconds ...
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bioRxiv - Molecular Biology 2023Quote: ... Each sample was homogenised in 1 mol.L-1 HCl (VWR, United States). Free glucose content was measured at this stage ...
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bioRxiv - Immunology 2023Quote: ... HOIP (VWR/Proteintech 16298-1-AP, SHARPIN (VWR/Proteintech 14626-1-AP), and actin (Sigma-Aldrich A5316 ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was homogenized in 1 mol.L-1 HCl (VWR, United States) and free glucose content was measured ...
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bioRxiv - Genetics 2021Quote: ... 1 mM Squalene (VWR), 50 µM Lanosterol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% BSA (421501J, VWR), 0.1% Triton-X and 0.05% Tween-20 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MgCl2 (VWR), 1% Triton X-100 (Alfa Aesar) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% penicillin-streptomycin (VWR, 5 units/mL penicillin ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM dithiothreitol (VWR) in 384-well plates (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM dithiothreitol (VWR) in 384-well plates (Corning ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... 1 cover glass (VWR) was used for the top plate and a 40 mm cover glass (Fisherbrand ...
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bioRxiv - Neuroscience 2023Quote: ... brains were kept for 1 hour in a 1:1 solution of 30% sucrose and Optimal Cutting Temperature Medium (OCT, VWR International) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with the kit TaqPolimerase 2X (VWR) using the following PCR program ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Minipreps were performed using a peqGold miniprep kit (VWR). WMISH probes were generated via a SP6 or T7 transcription kit (NEB ...
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bioRxiv - Genomics 2024Quote: The peqGOLD Tissue DNA Mini Kit (VWR Life Science) was used to extract genomic DNA after genome editing in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Biophysics 2020Quote: ... the untreated and treated bacteria (expressing H-NS-mEos3.2 fusion proteins) were filtered by 0.2 μm filters (VWR International LLC), resulting in filtrates constituting leaked cellular contents due to membrane damage ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The dissected cuticle was sandwiched between two #1 coverslips (VWR #1 16004-094) with ~10 μL of UV curable resin (Bondic UV glue #SK8024 ...
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bioRxiv - Biochemistry 2024Quote: ... in a ratio of 1:10 in 1 mL of dichloromethanol (VWR BDH23373.400) and 50 μL of pyridine (Sigma-Aldrich #270970 ...
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bioRxiv - Cancer Biology 2023Quote: ... RPMI1640 complete media (1% FBS, 1× penicillin/streptomycin (VWR, Cat. No. CA45000-650), 1 μg/mL dexamethasone (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 μg of either wild-type FHL-1 or RGD-null FHL-1 was incubated with 2 μg C3b (VWR International, Lutterworth, UK), and 0.4 μg factor I (FI ...
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... a 1:1 ratio of 0.6% agarose and 2X EMEM (VWR, Cat # 10128-758) supplemented with 5% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated 10 min (1:4000, stock 1 mg/ml, VWR, Vienna, Austria) followed by rinsing in PBS (three times 5 min) ...
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bioRxiv - Cancer Biology 2021Quote: ... or DMSO (VWR, 1:1000) as vehicle-control ...