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Citations for VWR :
351 - 400 of 1169 citations for Rat G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... Soil (10 g) was suspended in deionized water (50 mL) and mixed on an orbital shaker (OS-500, VWR) for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... Sorbarod filter samples were homogenised via placement in half-strength thioglycolate medium (14.5 g/L; Oxoid, Basingstoke, UK) and 1g of 3.5-5.5mm sterile glass beads (VWR, Lutterworth, UK). Samples were vortexed (30 seconds) ...
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bioRxiv - Microbiology 2023Quote: ... 8% CO2 with continuous shaking and culture supernatant was then clarified by centrifugation at 4000 x g for 20 minutes and filtered using 0.2 μm vacuum filtering system (VWR). SOSIP glycoproteins were purified from the supernatant by affinity chromatography and further by size exclusion chromatography as follows ...
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bioRxiv - Microbiology 2024Quote: Bacterial load in faeces was determined using approximately 500 µL of frozen faecal slurry (238 – 816 mg) and diluting it 400,000 times in physiological solution (8.5 g/L NaCl; VWR International). Next ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... HPLC grade Acetonitrile was purchased from P212121 (Ann Arbor, MI) and HPLC grade trifluoroacetic acid was purchased from VWR (Radnor, PA). The water used in all purifications and experiments was obtained from a MilliQ system and had a minimum resistivity of 18 MΩ ...
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bioRxiv - Microbiology 2020Quote: ... Cells were covered with Eagle’s Minimum Essential Medium (without phenol red, supplemented with 5% FBE, non-essential amino acids, 1mM sodium pyruvate (VWR, 45000-710), 2mM L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were diluted with ultrapure water to a final concentration of 0.475 N nitric acid and transferred to metal-free centrifuge vials (VWR, 89049-172) for subsequent ICP-MS analyses.
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bioRxiv - Plant Biology 2020Quote: ... and all protein estimations were done using Bradford reagent (VWR Inc.).
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bioRxiv - Microbiology 2020Quote: ... Protein syntheses were induced by 2% (wt/vol) galactose (VWR International) at 30 °C for 4 h at 250 rpm when the OD600 reached 0.8–1.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to a PVDF membrane (VWR, Cytiva, #10061-492), blocked with 5% powdered milk in TBST (50 mM Tris-HCl pH7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1% Rho-associated protein kinase (ROCK) inhibitor (Y-27632; VWR). Each EHT construct consisted of 1.0 x106 cells ...
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bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.1 mg/mL bovine serum albumin protein (VWR, 97062-508), then 1 hour with Pierce™ Protein A/G agarose beads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred to nitrocellulose blotting membranes (10-6000-01, VWR) in Harlow buffer at 350mV and 4°C for 2 hours ...
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bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
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bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... 0.4 g of poly(FATRIFE-ter-PEGMA-ter-NBMA) terpolymer was dissolved in 25ml methyl-ethyl ketone (25642.325, VWR, UK) in a 500 ml rotary evaporator flask containing 20 g of FEP at 40 °C to coat FEP particles ...
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bioRxiv - Biophysics 2019Quote: ... The reactions were quenched by the addition of SDS-PAGE loading buffer and analyzed by 10% Tricine-SDS-PAGE and stained with Coomassie Brilliant Blue G-250 (VWR).
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bioRxiv - Physiology 2020Quote: ... containing 10% serum and centrifuged at 50 g for 5 min to enrich hepatocytes and passed through a 100-micron nylon cell strainer (10199-659, VWR) multiple times ...
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bioRxiv - Biophysics 2022Quote: ... each flowcell consists of a set of five parallel microchannels that are sandwiched between a quartz microscope slide (G. Finkenbeiner) and a borosilicate glass coverslip (VWR). Prior to assembling the flowcell ...
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bioRxiv - Microbiology 2023Quote: ... were generated by centrifugation of the grown culture at 5800 x g for 20 min and subsequent filter-sterilization (0.2 µm, VWR™). SM samples were prepared freshly before each experiment under anaerobic conditions ...
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 7 cm diameter column loaded with 20 g of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the mobile phase ...
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bioRxiv - Neuroscience 2023Quote: ... and the sections were finally mounted under glass coverslips with Fluoromount-G (Cat No. 100241-874, VWR, Radnor, PA, USA).
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bioRxiv - Genomics 2024Quote: ... supplemented with PIM(ABS) (Prodo Labs) and PIM(G) (Prodo Labs) and incubated in a T-150 non-tissue culture treated flask (VWR) for recovery at 37°C and 5% CO2 overnight ...
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bioRxiv - Genomics 2019Quote: ... then centrifuged at 4000 x g for ten minutes and filtered the supernatant through a Poly-ethersulfone filter (VWR, 28145-505) to remove cellular debris ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was centrifuged at 14000×g for 40 min and a Macrosep advanced filter (3000 Da MW cutoff, 20 ml; catalogue no. 89131-974; VWR, USA) was used to remove the free biotin ...
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bioRxiv - Biochemistry 2023Quote: ... Each sample (10 μL) was loaded and separated on an SDS-PAGE followed by Coomassie G-250 staining (VWR, Cat. # 0615) to visualize the overexpression of the desired fusion protein.
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bioRxiv - Molecular Biology 2023Quote: ... the suspension centrifuged for five minutes at 300 x g again and the resulting pellet flash frozen in dry ice and Methanol (VWR, 20847.307). Pellets were thawed on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Physiology 2023Quote: ... 8 μl of the final polyacrylamide mix were pipetted on 35 mm glass bottom dishes (Cell E&G LLC VWR, USA) which were pretreated with 0.1 M NaOH ...
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bioRxiv - Cell Biology 2021Quote: ... Separated proteins were transferred onto 0.45 μm nitrocellulose transfer membranes (VWR International). Protein loading and transfer quality were checked by staining with Ponceau S ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein was isolated using Ni-NTA Resin (VWR Cat. No. 786-940) and eluted with 2 mL of elution buffer (300 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were dialyzed in a Slide-a-lyzer (VWR, 514-0172) overnight in ATPase buffer (100mM potassium acetate ...
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bioRxiv - Microbiology 2019Quote: ... Insoluble protein pellets were resolubilized in 3 M urea (VWR, Radnor, PA) and purified using immobilized metal affinity chromatography using Ni-NTA resin (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
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bioRxiv - Cell Biology 2022Quote: ... Denatured proteins were then transferred to a Nitrocellulose membrane (Amersham Protran, VWR) by applying 300 mA for at least 60 min in a wet transfer chamber with blotting buffer (25 mM Tris pH 8.3 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... At least 100 embryos stored in methanol: acetic acid glacial were dropped onto an uncoated and clean microscope slide (VWR™, 631-1550) and let dry on a wet paper for 30 min ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 1x105 neurons/ml so that 1x105 neurons were seeded onto poly-D-lysine (10 µg/mL) coated acid-etched coverslips (VWR,, cat#MSPP-P06G1520F) and cultured at 37°C plus 5% CO2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each bone was then cut in half and transferred to a 1.5 ml microcentrifuge tube containing a G-Tube microcentrifuge tube (VWR, Radnor, PA, USA) that had been punctured at the bottom with a 15 gauge needle ...
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bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with retroviral Gag-pol (8 ug) and VSV-G (4 ug) packaging plasmids and pBabe vector (8 ug) using PEI Max transfection reagent (VWR, 75800-188). After 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Mycobacterium smegmatis (ATCC 700084) was maintained in 7H9 media supplemented with 10% ADS enrichment: 50 g bovine serum albumin (VWR, Radnor, PA), 20 g dextrose (Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
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bioRxiv - Cell Biology 2024Quote: ... The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR: 27376-991). Membranes were blocked in 5% nonfat dairy milk in TBS-T ...
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bioRxiv - Microbiology 2021Quote: ... was used to stain nucleic acids following washing away unbound secondary antibodies and samples were covered with VectaShield anti-fade reagent (Vector Laboratories, VWR, Cat# 101098-048) and a coverslip ...
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bioRxiv - Microbiology 2019Quote: ... Solution B comprised 30 μl of solution A supplemented with 2 μl of a solution prepared by dissolving 120 mg of phenyl boronic acid in 3 ml dimethyl sulfoxide (VWR International catalog # BDH1115-1LP) and adding this to 3 ml sterile inoculum water (Beckman Coulter ...