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Citations for VWR :
151 - 197 of 197 citations for Rat Androgen dependent TPF1 regulating protein ADTRP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... DNA extracted using a PeqGold DNA Blood and Tissue Extraction kit following the manufacturer’s instructions (VWR), and PCR performed targeting both Ca ...
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bioRxiv - Developmental Biology 2023Quote: ... The 3’ end enriched libraries were constructed using KAPA HyperPlus Library Preparation Kit (VWR International, KK8513) with 6 cycles of PCR ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was isolated from cells using the EZNA Total RNA Kit (Omega, VWR, Radnore, PA, USA). 1 μg of RNA was reverse-transcribed into cDNA using the RT Omniscript cDNA kit (Qiagen) ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from ∼50 mg samples with an E.Z.N.A® Plant DNA Kit (VWR, Lutterworth, UK). DNA fragment size was assessed on a 1% agarose gel in Tris/Borate/EDTA to confirm that all samples had the majority of DNA fragments >10 kilobases.
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated from tissue or cell culture using the EZNA RNA isolation Kit (VWR, Spånga, Sweden) or using TRIzol reagent and RNeasy Universal Mini Kit (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Trichrome staining was performed on 5 μm tissue sections using Gomori’s Trichrome Stain Kit (VWR, 84000-308) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted using the peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) for lines transformed with pOsCPR5.1-B construct while extraction was done according to (Edwards et al. ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Total RNA from cells was isolated using the peqGOLD total RNA isolation kit (VWR, Radnor, PA, USA) according to the manufacturer’s protocol and RNA concentrations of the samples were determined with a NanoDrop 1,000 spectrometer (VWR ...
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bioRxiv - Plant Biology 2021Quote: Leaf genomic DNA was isolated using a peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was lysed with TRK lysis buffer of the Micro Elute Total RNA Kit (VWR International PBI) and left rotating on a wheel for 20 min at room temperature ...
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bioRxiv - Genetics 2024Quote: ... The DNA was isolated from frozen ear muscle biopsies using the peqGOLD blood & tissue DNA mini kit (VWR) and following the protocol provided by the manufacturer.
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bioRxiv - Microbiology 2024Quote: Shotgun sequencing libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (VWR International, Radnor, USA), and sequenced with PE150 on an Illumina NovaSeq6000.
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bioRxiv - Molecular Biology 2024Quote: ... The amplification conditions were adjusted using foliar DNA isolated with the Plant DNA mini kit (VWR, Darmstadt, Germany). Thus ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated using the Qiagen QuantiTect Reverse Transcription Kit and analyzed using the PerfeCTa SYBR green FastMix (VWR) and a Rotor-Gene 6000 (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
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bioRxiv - Genomics 2020Quote: Library preparation for each CUT&RUN antibody condition was done with a KAPA Hyper Prep Kit (VWR, 89125-040) with the PCR amplification settings adjusted to have simultaneous annealing and extension steps at 60 °C for 10 seconds ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated and cDNA was synthesized with the peqGOLD MicroSpin Total RNA Kit (Peqlab/VWR Life Science, 12-6831) and the qScript™ Flex cDNA Kit (Quantabio ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was prepared from excised root tips (tip growth gained after seedling transfer) by using the peqGOLD Plant RNA Kit (VWR). One biological replicate represents 40-60 pooled root tips ...
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bioRxiv - Molecular Biology 2021Quote: ... 1ug of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... 1µg of RNA was reverse transcribed by oligo-dT and random priming into cDNA with a qScript cDNA SuperMix kit (QuantaBio-VWR), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Tissues were homogenized with a Minilys homogenizer (Bertin Technologies, Rockville, MD) using a Precellys Lysing Kit (VWR Scientific, Radnor, PA). Total RNA was extracted from thoroughly homogenized tissues via miRNeasy Mini Kit (#217004 ...
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bioRxiv - Immunology 2020Quote: ... Approximately 250-500ng RNA was normalized across samples and cDNA was generated using the qScript cDNA Synthesis Kit (101414-098, VWR). cDNA was then subjected to qPCR using PerfecTa SYBR Green FastMix (84069 ...
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bioRxiv - Developmental Biology 2022Quote: RNA from embryonic kidneys was extracted by the use of the peqGold Total RNA-Kit (VWR International GmbH, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2019Quote: ... total iron binding capacity (TIBC) and transferrin saturation were measured using the Stanbio Iron & TIBC kit (VWR, cat. number 10152-550). Serum ferritin was quantified using the mouse ferritin ELISA kit (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... a part of the organ tissue was weight and homogenised following the protocol of the Precellys soft tissue lysing kit (CK14, Bertin Instruments, VWR, UK). The fluorescence of Cy7-psomes was measured in using a multimode microplate fluorometer (Spark ...
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bioRxiv - Genetics 2020Quote: ... centrifuged and the aqueous phase was applied to a PerfectBind RNA Colum of the Total RNA Kit (peqGOLD, VWR, Radnor, USA). Further extraction of total RNA was performed according to the kits protocol ...
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bioRxiv - Physiology 2022Quote: ... The resulting aqueous phase was transferred to a Perfect Bind RNA Colum of the Total RNA Kit (peqGOLD, VWR, Radnor, USA), following the kit’s protocol for purification ...
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bioRxiv - Molecular Biology 2022Quote: ... following the instructions of the manufacturer with minor modifications (according to Exiqon’s Guidelines for ISH on fresh frozen material using the miRCURY LNA ISH Optimization kits) and using Pertex (VWR, Stockholm, Sweden) for mounting ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We inoculated these 16 samples in YEPD for 12 hours and extracted their genomic DNA using a commercially available kit (Amresco, Inc. VWR USA). We measured the concentration of DNA with a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Evolutionary Biology 2019Quote: ... reverse transcription was performed using 4.0 μl of 10.0 μl total RNA solution with the cDNA-Synthesis Kit H Plus (VWR Peqlab, Germany, 732-3273). CFX Connect™ Real-Time PCR Detection System (BioRad ...
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Genomics 2024Quote: Total RNA of minigene-transfected HEK293T cells and patient-derived fibroblasts was extracted using the peqGOLD Total RNA Kit (VWR Life Science). One microgram of RNA was treated with 1 U of DNaseI (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... We then used a glass slide to touch each region of the inside of the reproductive tract and stained these slides with a modified Giemsa stain (Differential Quik Stain Kit, VWR, Radnor PA) to look for the presence of sperm and inflammatory cells ...
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bioRxiv - Microbiology 2020Quote: ... Synthesis of the first-strand cDNA from the extracted total RNA was conducted using a QuantaBio qScript cDNA synthesis kit (VWR Canada, Edmonton, AB). Primers and gBlocks were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Bioengineering 2024Quote: ... PCLS were then placed on Gelfoam® for long-term culture and monitored using a WST-8 cell counting kit (VWR #89155-898) according to the kit’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... were added directly to the Sterivex filter with lysis buffer from the All-prep® kit and mixed on a vortex mixer (VWR model VM-3000) for 20 minutes at the maximum setting ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single colonies were grown overnight in 10 mL YPGal+Dex and genomic DNA was isolated from 1 mL aliquots using the VWR® Life Science Yeast Genomic DNA Purification Kit (VWR 89492-616, us.vwr.com).
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bioRxiv - Microbiology 2024Quote: Plasmids and corresponding oligonucleotides used in this study are summarized in Supplementary Tables 1 and 2. For DNA preparations, the appropriate enzymes (Fermentas, St. Leon-Rot, Germany) and kits (VWR International GmbH, Darmstadt, Germany) were used ...
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bioRxiv - Genomics 2023Quote: ... (Lexogen SPLIT RNA kit) was added to each tube and the tissue homogenized using a plastic pestle (Kontes Pellet Pestle, VWR, catalogue number: KT749521-1500). Samples remained on ice to maintain RNA integrity while other samples were homogenized ...