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Citations for VWR :
101 - 150 of 194 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Bioengineering 2021Quote: ... 100 μL of dye at each concentration were aliquoted into a strip of 12 optically-clear PCR tubes (VWR 93001-118). The instrument was allowed to reach a steady-state temperature of 69° C before the first strip of tubes was loaded ...
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bioRxiv - Bioengineering 2021Quote: ... antibiotic-resistant colonies were re-streaked and verified by colony PCR using specific primers and Red Taq DNA polymerase master mix (VWR International).
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bioRxiv - Microbiology 2023Quote: ... Then all PCR products were run on 1.5% agarose gel and the PCR product concentrations were assessed based on gel band intensity using GENOSOFT software (VWR International, Belgium). By their concentrations ...
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bioRxiv - Biophysics 2023Quote: ... the top and bottom strand were annealed using a PCR machine (Doppio, VWR®-thermocyclerseries, VWR, part of Avantor, Radnor, US) increasing the temperature to 95 °C (3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were visualised on a 1% agarose gel (run for 30 minutes at 135 V) with GelGreen (VWR, 730-1535) using an ImageQuant™ LAS 500 (GE Lifesciences) ...
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bioRxiv - Microbiology 2024Quote: ... An aliquot (24.5 µL) of the master mix was added to all wells of a 96-well PCR plate (VWR, Radnor, PA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Minipreps were performed using a peqGold miniprep kit (VWR). WMISH probes were generated via a SP6 or T7 transcription kit (NEB ...
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bioRxiv - Genomics 2024Quote: The peqGOLD Tissue DNA Mini Kit (VWR Life Science) was used to extract genomic DNA after genome editing in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Digestion and ligation efficiencies were assessed by gel electrophoresis on a 0.9% agarose gel and quantitative PCR (Brilliant III SYBR qPCR Master Mix, VWR Cat # 97066-528).
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bioRxiv - Bioengineering 2021Quote: ... A 15-µl aliquot of the master mix was combined with 10 µl of sample in a 96-well PCR plate (VWR, 83007-374). The plate was mixed on a Qiagen TissueLyser II for two 25-second intervals at 13 Hz then centrifuged for 2 minutes at 1000 xg.
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were treated either with 800 μM OPA or corresponding concentration of DMSO and heated in parallel in a PCR (VWR, Doppio Gradient) block for 15 min to the three temperatures (37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated using the peqGOLD Total RNA Kit (VWR) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... were prepared and stored in 0.2 mL snap strip PCR tubes with dome caps (490003-692, GeneMate – BioExpress, VWR International, Brisbane, CA, USA) at -20 °C prior to qRT-PCR plates preparation ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated using TriFast RNA extraction kit (30-2030, VWR) or RNA-Solv reagent (Omega Bio-tek) ...
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bioRxiv - Genomics 2023Quote: DNA was purified using the yeast genomic DNA purification kit (VWR). Briefly ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA Kit I (Omega BIO-TEK; VWR catalog no. 101319) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The COD was determined using the Spectroquant COD kit (VWR, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... extracted with the RNA extract kit (1x106 cells-10mg; VWR International PBI) and retrotranscribed with iScript Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted using the Zymo Quick-RNA MiniPrep kit (VWR, R1055) according to the manufacturers protocol ...
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bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... Total DNA was isolated using Plant DNA mini Kit (Peqlab/VWR, Darmstadt, Germany). To quantify fungal or bacterial DNA content ...
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bioRxiv - Microbiology 2021Quote: ... histolytica expression plasmid pEhEx backbone (38) using the Gibson Assembly Ultra Kit (VWR). Primers used for Gibson cloning are described in Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted using the Zymo Quick-RNA miniprep kit (VWR; R1055) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted using the Direct-zol RNA miniprep kit (VWR 76211-340). cDNA synthesis was performed using hexamer primers and SuperScript IV Reverse Transcriptase (Thermo Fisher 18090010) ...
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bioRxiv - Molecular Biology 2022Quote: ... The total soma RNA was extracted with the RNA extraction kit (VWR International PBI) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... DNA was extracted from the gel slices using the peqGOLD Gel Extraction Kit (VWR). Genotyping was done using a modified ast-PCR protocol for R21X (Supplemental methods ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription was performed using the qScrpit cDNA synthesis kit (VWR, catalog # 95048-100) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the DNA using the Life Science Yeast Genomic DNA Purification Kit (VWR). We sent the samples for library preparation and sequencing at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was isolated from the cells with Zymo Quick-RNA Miniprep kit (VWR, #76299-632) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Immunology 2021Quote: ... The pool was quantified using the Peqlab KAPA Library Quantification Kit (VWR, Radnor, PA; USA) and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were monitored for Mycoplasma contamination regularly using Mycoplasma Detection Kit (VWR, 89510-164).
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bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
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bioRxiv - Immunology 2021Quote: ... The pool was quantified using the Peqlab KAPA Library Quantification Kit (VWR, Radnor, PA; USA) and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... 5ml of curing agent and 45ml of Monomer (both Sylgard® 184 Elastomer Kit, VWR) were mixed intensively ...
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bioRxiv - Neuroscience 2022Quote: Frozen corpus callosa were pulverised using the Precellys Bioruptor and bead-based lysis kit (VWR). Tissue was transferred to 1.5ml Precellys beaded tubes with 300 μl of lysis buffer (5% SDS ...
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bioRxiv - Physiology 2022Quote: ... the isolation was performed according to the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA) and verified through sequencing.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... by grinding the larvae in the C1 buffer of this kit with a Precellys (VWR).
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bioRxiv - Cell Biology 2022Quote: Total RNA was prepared using the peqGOLD total RNA isolation kit (VWR, Radnor, PA, USA) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (VWR 101414). Technical replicates of 20 ng (based on RNA concentration ...
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bioRxiv - Bioengineering 2020Quote: ... A temperature-controlled oil bath (Instatherm® Economy Bath/Controller Kit, Ace Glass, Inc., VWR, USA) equipped with a type J thermocouple temperature sensor was employed to control the heat treatment temperature ...