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Citations for VWR :
101 - 150 of 165 citations for RNA Amplification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... RNA probes were stored in 25% formamide/2x saline-sodium citrate (25% FA/2x SSC; VWR Chemicals, Leicestershire, UK) at −20°C for long-term storage.
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and aqueous separation of RNA was performed via centrifugation in a heavy gel phase-lock tube (VWR, 10847--802). The aqueous phase was collected ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were generated by incubating the RNA samples in a solution containing qScript cDNA SuperMix (VWR #101414-102) and nuclease-free water ...
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bioRxiv - Molecular Biology 2020Quote: ... Subsequently the aqueous phase was tranferred to a new tube and RNA precipitated with isopropanol (0.5 mL per 1 mL of trizol, VWR, Denmark) by incubation at −20 °C for two hours followed by cenrifugation (12 000 g ...
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bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s protocol and RNA concentrations of the samples were determined with a NanoDrop 1,000 spectrometer (VWR, Darmstadt, Germany). 1 µg of RNA per sample was used for cDNA synthesis with the RevertAid H Minus First Strand Kit (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Injection mixture was prepared on ice containing 300 ng Tol2 transposase RNA and 500 ng recombinant plasmid and was brought to 10 µl total volume with 0.1% phenol-red (VWR, 470301-974) in water ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a ratio of 1:5 (chloroform:trizol) for aqueous separation of RNA using heavy gel phase-lock tubes (VWR, 10847-802). Library preparation and RNA-sequencing was performed by Genewiz (Azenta Life Sciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were plated in 24 well CELLSTAR® cell culture plates (for bulk-RNA-seq. and live imaging; VWR, 82050-892), µ-Slide 8 Well (for Video S1. ...
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bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... Total DNA was isolated using Plant DNA mini Kit (Peqlab/VWR, Darmstadt, Germany). To quantify fungal or bacterial DNA content ...
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bioRxiv - Microbiology 2021Quote: ... histolytica expression plasmid pEhEx backbone (38) using the Gibson Assembly Ultra Kit (VWR). Primers used for Gibson cloning are described in Table 1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were carried out using the KAPA3G Plant PCR Kit (Kapa Biosystems, VWR) using the following cycling conditions for the phylogenetically informative loci ...
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bioRxiv - Physiology 2022Quote: ... frozen and crushed in liquid nitrogen for protein and RNA extraction or ii) embedded in Tissue-Tek OCT compound (VWR, Mississauga, Canada) and frozen in isopentane cooled with liquid nitrogen for cryostat sectioning54 or iii ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Cell Biology 2023Quote: ... 300 µL of chloroform was added to the sample and aqueous separation of RNA was performed using centrifugation in a heave gel phase-lock tube (VWR, 10847-802). The aqueous phase was then applied to a standard RNA purification kit (Quantabio ...
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bioRxiv - Genetics 2019Quote: ... DNA was extracted from the gel slices using the peqGOLD Gel Extraction Kit (VWR). Genotyping was done using a modified ast-PCR protocol for R21X (Supplemental methods ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 30 minutes at RT and Vector VIP Peroxidase Substrate Kit (VWR) was used to develop staining ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription was performed using the qScrpit cDNA synthesis kit (VWR, catalog # 95048-100) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted the DNA using the Life Science Yeast Genomic DNA Purification Kit (VWR). We sent the samples for library preparation and sequencing at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from the eluted beads with phenol: chloroform: iso–amyl alcohol (25:24:1) (v/v/v) (VWR, 136112– 00–0), followed by precipitation with 1:1 (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Immunology 2021Quote: ... The pool was quantified using the Peqlab KAPA Library Quantification Kit (VWR, Radnor, PA; USA) and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were monitored for Mycoplasma contamination regularly using Mycoplasma Detection Kit (VWR, 89510-164).
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bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
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bioRxiv - Immunology 2021Quote: ... The pool was quantified using the Peqlab KAPA Library Quantification Kit (VWR, Radnor, PA; USA) and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... 5ml of curing agent and 45ml of Monomer (both Sylgard® 184 Elastomer Kit, VWR) were mixed intensively ...
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bioRxiv - Neuroscience 2022Quote: Frozen corpus callosa were pulverised using the Precellys Bioruptor and bead-based lysis kit (VWR). Tissue was transferred to 1.5ml Precellys beaded tubes with 300 μl of lysis buffer (5% SDS ...
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bioRxiv - Physiology 2022Quote: ... the isolation was performed according to the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA) and verified through sequencing.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... by grinding the larvae in the C1 buffer of this kit with a Precellys (VWR).
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bioRxiv - Cell Biology 2024Quote: ... and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (VWR 101414). Technical replicates of 20 ng (based on RNA concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... Stellaris® RNA FISH Wash Buffer B was removed and samples were resuspended in Vectashield Antifade Mounting Medium with DAPI (VWR; Cat #: H-1200-10). Samples were then placed on a slide and sealed with a coverslip and nail polish.
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bioRxiv - Bioengineering 2020Quote: ... A temperature-controlled oil bath (Instatherm® Economy Bath/Controller Kit, Ace Glass, Inc., VWR, USA) equipped with a type J thermocouple temperature sensor was employed to control the heat treatment temperature ...
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bioRxiv - Microbiology 2023Quote: ... DNA extracted using a PeqGold DNA Blood and Tissue Extraction kit following the manufacturer’s instructions (VWR), and PCR performed targeting both Ca ...
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bioRxiv - Developmental Biology 2023Quote: ... The 3’ end enriched libraries were constructed using KAPA HyperPlus Library Preparation Kit (VWR International, KK8513) with 6 cycles of PCR ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was extracted from ∼50 mg samples with an E.Z.N.A® Plant DNA Kit (VWR, Lutterworth, UK). DNA fragment size was assessed on a 1% agarose gel in Tris/Borate/EDTA to confirm that all samples had the majority of DNA fragments >10 kilobases.
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bioRxiv - Cell Biology 2021Quote: ... Trichrome staining was performed on 5 μm tissue sections using Gomori’s Trichrome Stain Kit (VWR, 84000-308) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted using the peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) for lines transformed with pOsCPR5.1-B construct while extraction was done according to (Edwards et al. ...
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bioRxiv - Plant Biology 2021Quote: Leaf genomic DNA was isolated using a peqGOLD Plant DNA Mini Kit (PeqGold, VWR International GmbH, Darmstadt, Germany) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The DNA was isolated from frozen ear muscle biopsies using the peqGOLD blood & tissue DNA mini kit (VWR) and following the protocol provided by the manufacturer.
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bioRxiv - Microbiology 2024Quote: Shotgun sequencing libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (VWR International, Radnor, USA), and sequenced with PE150 on an Illumina NovaSeq6000.
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated using the Qiagen QuantiTect Reverse Transcription Kit and analyzed using the PerfeCTa SYBR green FastMix (VWR) and a Rotor-Gene 6000 (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
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bioRxiv - Genomics 2020Quote: Library preparation for each CUT&RUN antibody condition was done with a KAPA Hyper Prep Kit (VWR, 89125-040) with the PCR amplification settings adjusted to have simultaneous annealing and extension steps at 60 °C for 10 seconds ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...