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Citations for VWR :
1 - 50 of 229 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined with Bradford reagent (VWR, E530-1L). Western blotting was performed as previously described (43 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration throughout the study was determined via Bradford assay (catalogue number-VWR M172).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The dots were normalized on protein concentration (BCA assay) and cell numbers (EVE Automated Cell Counter, VWR). Each drop contained 10ug protein in 5 ul RIPA ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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bioRxiv - Cancer Biology 2020Quote: ... high concentration rat-tail collagen I (VWR) was neutralized with a 1:1 ratio of 100 mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Sodium salicylate (1 μM final concentration; VWR) was added for induction of traD ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... Beads were then split into tubes and MMP assay +/− activated MMP (final concentration 0.73 ug/mL unless noted otherwise) and +/− Batimastat (final concentration 40 uM) (BB94, VWR) as needed for that condition ...
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bioRxiv - Bioengineering 2020Quote: ... either D-luciferin (Fisher #LUCK-2G; 150 µg / mL read concentration) or Rluc substrate (VWR # PAP1232; 17 µM read concentration). Maximum cytotoxicity was defined as luminescent signal from wells containing only media while no cytotoxicity was defined by wells containing only target cells ...
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bioRxiv - Molecular Biology 2023Quote: ... the concentration of organic solvent (acetonitrile, VWR, 83639.320) was increased from 1.6% to 32% in 0.1% formic acid at a flow rate of 230 nl / min ...
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bioRxiv - Molecular Biology 2023Quote: ... the concentration of organic solvent (acetonitrile, VWR, 83639.320) was increased from 1.6% to 32% in 0.1% formic acid at a flow rate of 230 nl / min ...
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bioRxiv - Immunology 2023Quote: ... the concentration of organic solvent (acetonitrile, VWR, 83639.320) was increased from 1.6% to 32% in 0.1% formic acid at a flow rate of 230 ml/min ...
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bioRxiv - Immunology 2023Quote: ... the concentration of organic solvent (acetonitrile, VWR, 83639.320) was increased from 1.6% to 28% in 0.1% formic acid at a flow rate of 230 nl / min ...
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bioRxiv - Genetics 2020Quote: ... Cell lysate concentrations were measured using Bradford reagent (VWR) or BCA assay (ThermoFisher) ...
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bioRxiv - Biochemistry 2020Quote: ... The Click reaction contained (final concentrations) 1% SDS (VWR), 5% t-butanol (Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... and sucrose (VWR, final concentration of 10%, wt./vol.) added prior to pouring plates.
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bioRxiv - Plant Biology 2021Quote: ... samples were dehydrated with increasing concentrations of acetone (anhydrous, VWR) and embedded in epoxy resin TAAB 812 (TAAB Laboratories) ...
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bioRxiv - Microbiology 2024Quote: ... mRNA concentrations were determined using a Nanodrop (Peqlab VWR, USA) and 20 ng were used for reverse transcription using the RevertAid H Minus First Strand cDNA Synthesis kit (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Immunology 2020Quote: ... cells were fixed with a final concentration of 3% PFA (VWR) in medium for 10 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were prepared at 10 mM stock concentration in DMSO (#23486, VWR Chemicals) and stored at −20°C ...
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bioRxiv - Cell Biology 2021Quote: ... Auxin plates contained a final concentration of 1mM auxin (VWR Cat#AAA10556-36) prepared from a 500mM stock in ethanol (Zhang et al ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Microbiology 2022Quote: ... to obtain a final concentration of 1 μM and sodium nitrate (SS0680-500GR VWR) to obtain a final concentration of 10 μM.
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bioRxiv - Neuroscience 2022Quote: ... containing three different concentrations of EtOH (5%, 10%, 20%, prepared using 96% EtOH; VWR international #83804.360 diluted to the correct concentration ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Neuroscience 2020Quote: ... Odor concentrations are reported as v/v dilution in paraffin oil (J.T. Baker, VWR #JTS894), with the exceptions of ammonia and acetic acid ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 20% trichloroacetic acid (TCA – VWR) on ice overnight at 4° C ...
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bioRxiv - Physiology 2021Quote: 6xHis fusion proteins were grown in M15 cells (VWR) and prepared as above except that after IPTG induction ...
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bioRxiv - Biochemistry 2023Quote: ... Staining was carried out with InstantBlue Protein Stain (VWR).
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bioRxiv - Cell Biology 2020Quote: NGM plates with a final concentration of 1μM auxin (indole-3-acetic acid, VWR AAA10556-36) were made according to [60] ...
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bioRxiv - Neuroscience 2023Quote: All odor concentrations are reported as v/v dilutions in paraffin oil (J.T. Baker, VWR #JTS894), or autoclaved and twice-filtered distilled water (for diluting acids) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4.5 µM carbonyl cyanide-p-trifluoromethoxyphenylhydrazone for a final in-well concentration 0.5µM (“FCCP,” VWR Scientific), and a 100 µM mix of Rotenone (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: The MIC and minimum bactericidal concentrations (MBC) of benzalkonium chloride (BZC) (CAS 68391-01-5, VWR) were determined for representative sequenced isolates (n=45 ...
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bioRxiv - Systems Biology 2021Quote: ... 50 ng lysozyme and PMSF (final concentration of 1 mM) and sonication (Branson Sonifier 450, VWR international). Centrifugation was used to separate soluble proteins from cell debris ...