Labshake search
Citations for VWR :
51 - 100 of 879 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
-
bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
-
bioRxiv - Neuroscience 2021Quote: ... primary antibodies (1:1000 goat anti-GFP from Novus Biologicals or 1:10 000 rabbit anti-RFP from VWR (Rockland) were incubated with PGT overnight at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked in 5% milk and then probed with the indicated primary antibodies diluted in 5% bovine serum albumin (VWR). Blots were imaged after incubation with appropriate primary and secondary antibodies by scanning on an Odyssey IR imager (LI-COR) ...
-
bioRxiv - Biochemistry 2019Quote: ... spectra of native, human BACE1 (6.12 μM, 30 μl; Acro Biosystems, USA) in 50 mM sodium acetate (pH 4.0; VWR, UK) was obtained using a J-1500 Jasco CD spectrometer and Spectral Manager II software ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Human foreskin fibroblasts (HFF, kindly provided by Denise Galloway) were maintained in Minimum Essential Media-α (MEM-α; VWR) supplemented with 20% FBS (GE Healthcare ...
-
bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
-
bioRxiv - Microbiology 2019Quote: ... strains [69], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR) ...
-
bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
-
bioRxiv - Microbiology 2023Quote: Toxoplasma strain TATi-RHΔku80 [41] was cultured in human foreskin fibroblasts in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA, USA) supplemented with 10% fetal bovine serum (VWR) ...
-
bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were collected using a cell scraper (VWR) and resuspended in PBS containing 10% FBS and 1% sodium azide (Sigma) ...
-
bioRxiv - Neuroscience 2019Quote: Cryosections (20 – 40 μm) were generated using a cryostat (MICROM International, #HM560) on organoids and human retina embedded in OCT compound (VWR, #25608‒930). Sections were mounted onto Superfrost Plus slides (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were filtered through a 100µm cell strainer (VWR) and additional ...
-
bioRxiv - Biochemistry 2022Quote: ... Both cells were filtered through 40 μm cell strainer (VWR) prior to seeding ...
-
bioRxiv - Bioengineering 2020Quote: ... Cells were filtered through a 40 µm cell strainer (VWR) to remove cell clumps ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were detached with a cell scraper (VWR #10062-906) and the entire contents collected into a microcentrifuge tube ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were detached with a cell scraper (VWR, #734-2602), collected into a pre-chilled falcon tube in 3 repeated steps ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were then filtered using a 40 μm cell strainer (VWR) before cell sorting ...
-
bioRxiv - Bioengineering 2023Quote: ... The cells were cultured in T75 cell culture flasks (VWR International) and were only utilized or subcultured when they reached 85%–90% confluence.
-
bioRxiv - Cell Biology 2024Quote: ... and cells were detached with a cell scraper (VWR, #734-2602) and collected into a pre-chilled falcon tube in 3 repeated steps ...
-
bioRxiv - Immunology 2021Quote: ... Single cell suspensions for FACS staining were made by filtering of the cells through 40 µM cell strainers (734-2760, VWR).
-
bioRxiv - Biophysics 2021Quote: ... cells were plated at a density of 4×104 cells/cm2 in a 5-layer cell chamber (VWR 76045-402) to yield enough material for completing the experiment ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were filtered through a 40 µm cell strainer (VWR #10199-655) to ensure single cell suspension and analyzed using Sony SH800 flow cytometer at 488/535 nm excitation/emission ...
-
bioRxiv - Genomics 2020Quote: ... The cell viability and density was checked with Moxi Cell counter (VWR) and cells were diluted to achieve a density of 1 cell per 50 nl in a final dispensing mix which contained a diluent ...
-
bioRxiv - Bioengineering 2023Quote: ... A cell scraper (VWR) was used to resuspend the cells in the media ...
-
bioRxiv - Cell Biology 2019Quote: ... Then the cells were filtered through a 40-micron cell strainer (VWR 21008949) and diluted to the concentration of 100,000 cell/ml ...
-
bioRxiv - Immunology 2023Quote: ... The isolated cells were filtered through a 70 μM cell strainer and red blood cells were lysed with ACK lysis buffer (VWR; Radnor, PA, USA). Cells were then stained as described above for the bone marrow.
-
bioRxiv - Developmental Biology 2021Quote: ... dissociated into a single-cell suspension and filtered through a 40μm cell strainer (VWR). FACS analysis and cell sorting were performed using Fortessa and AriaIII instruments (Becton Dickinson) ...
-
bioRxiv - Cancer Biology 2019Quote: 8E5 cells LS1034 cells were seeded in 12-well plates (VWR International #29442-040) and incubated at 37C overnight ...
-
bioRxiv - Microbiology 2022Quote: ... cells were seeded at 30000 cells/cm2 in a 100 cm2 Petri dish (VWR). The next day ...
-
bioRxiv - Bioengineering 2021Quote: ... and cells were dissolved and detached with the help of a cell scraper (VWR). The samples were then frozen at −80 °C until further use ...
-
bioRxiv - Immunology 2020Quote: ... Cells were plated in a 96-well cell-culture treated plate (Costar, VWR, USA) at a density of 1×106 cells/mL in a 300 µL final volume ...
-
bioRxiv - Cell Biology 2021Quote: ... HeLa cells and HEK-293 cells were cultured on 22×22mm glass coverslips (VWR) in 6-well plates (Falcon) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell counts were obtained using a Moxi Z mini cell counter (VWR, Mississauga, Canada) and aliquots of 1 × 106 cells were placed into 2-mL cryovials (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cells were cultured in 100 mm cell culture dishes (CELLSTAR, VWR International, Mississauga, Canada) with Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells on glass coverslips (VWR) were covered with a polycarbonate filter (5 μm pore size ...
-
bioRxiv - Microbiology 2021Quote: ... blood cell counter vials (VWR) and 500 μl of a 10 ml resuspension of cells from each flask diluted with 19.5 ml Isoton II Diluent (Beckman) ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells on glass coverslips (VWR) were incubated 1 h at 37 °C in Duolink blocking buffer (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: Cell scraper (VWR 10062-904)
-
bioRxiv - Developmental Biology 2020Quote: ... The cell suspension was passed through a 40 µm Flowmi cell strainer (VWR, H13680-0040) and sorted on a BD FACSAria III or SONY SH800 cell sorter operated by the NIDCR Combined Technical Research Core.
-
bioRxiv - Genomics 2023Quote: ... the cell suspension was passed through a 70 μm cell strainer (VWR, Radnor, PA, USA) to remove large debris and cell clumps and centrifuged at 400g for 10 mins at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... cells were plated at 1×106 cells in a 24-well plate (VWR, 10861-558) and treated overnight with 20 μg/mL of soluble cholesterol ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were placed on dry ice and scraped with a cell lifter (#10062-904, VWR). The samples were transferred to a 1.5 mL tube and spun down with 15,000 g for 5 min at 4 °C ...
-
bioRxiv - Microbiology 2023Quote: ... cells were seeded at 1 x 105 cells/mL confluency in 1.5 glass coverslips (VWR) a day prior infection or drug treatment ...
-
bioRxiv - Microbiology 2023Quote: Cells were seeded at 1 x 105 cells/mL confluency in 1.5 glass coverslips (VWR) a day prior infection or drug treatment ...
-
bioRxiv - Immunology 2021Quote: ... Cells were detached completely by gently scraping with cell scraper with a cross-ribbed handle (VWR). Upon reaching 70-80% confluency ...
-
bioRxiv - Cell Biology 2019Quote: ... and 0.6 × 10^6 cells per temperature were seeded in T-25 cell culture flasks (VWR) in an appropriate volume of culture medium ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were filtered through a cell strainer (nylon mesh, 70 µm pore diameter, VWR, Radnor, USA) to remove cell clumps and were adjusted to 1×106 cells/mL before 1 µL of LIVE/DEAD Fixable Violet dead cell stain (Thermo Fisher Scientific ...